Hypoglycemic effect of Gynostemma pentaphyllum saponins by enhancing the Nrf2 signaling pathway in STZ-inducing diabetic rats

Gao, Dawei; Zhao, Min; Qi, Ximing; Liu, Yanping; Li, Nan; Liu, Zhiwei; Bian, Yanhong

doi:10.1007/s12272-014-0441-2

Cited by 54 publications

(33 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similar Nrf2 mediated protective effects against peroxide-induced toxicity have been reported in retinal epithelial cells (Alhasani et al, 2018). GYP treatment in a streptozotocin-induced diabetic rat model significantly upregulated Nrf2 expression and protected against oxidative stress (Gao et al, 2016). In agreement with these results, we have observed an increase in GLUTag cell viability following GYP treatment for 24h with upregulation of Nrf2 expression also noted in these cells along The proglucagon gene is expressed in both intestinal L-cells and pancreatic αcells, with post-translational processing of the propeptide by Pcsk1 generating GLP-1 in L-cells and Pcsk2 producing glucagon in α-cells (Drucker, 2006).…”

Section: Effects Of Gyp On Protein Expression and Glp-1 Stainingsupporting

confidence: 77%

Effects of gypenosides on enteroendocrine L-cell function and GLP-1 secretion

Patibandla

Campbell

Shu

et al. 2020

Preprint

View full text Add to dashboard Cite

Glucagon-like peptide 1 (GLP-1) is an incretin hormone produced in gut L-cells, which regulates postprandial glucose-dependent insulin secretion, also known as the incretin effect. GLP-1 secretion may be reduced in type 2 diabetes mellitus, impacting on glycaemic regulation. Thus, methods to enhance endogenous GLP-1 secretion by use of natural GLP-1 secretagogues may improve glucose control in diabetes. Gypenosides (GYP) extracted from the plant Gynostemma Pentaphyllum (Jiaogulan) are known for their glucose-lowering effects both in vitro and in vivo, although their effect on GLP-1 secretion is unknown. Our results showed that GYP enhanced cell viability and significantly upregulated antioxidant gene Nrf2, Cat and Ho-1 expression. GYP did not affect glucokinase expression but downregulated proglucagon gene expression over 24h, although, cellular GLP-1 content was unchanged. Prohormone convertase 1 (Pcsk1) gene expression was unchanged by GYP over 24h, although protein levels were significantly downregulated, while prohormone convertase 2 (Pcsk2) mRNA and protein levels were significantly upregulated. Acute exposure to gypenosides enhanced calcium uptake and GLP-1 release from GLUTag cells both at low and high glucose concentrations. These results suggest that anti-diabetic properties of gypenosides are partly linked to their ability to stimulate GLP-1 secretion. Gypenosides enhance antioxidant gene expression and may protect L-cells from excess oxidative stress.

show abstract

Section: Effects Of Gyp On Protein Expression and Glp-1 Stainingsupporting

confidence: 77%

Effects of gypenosides on enteroendocrine L-cell function and GLP-1 secretion

Patibandla

Campbell

Shu

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Nrf2 is the key molecule, which mediates the response of the endogenous antioxidant system ( Gao et al, 2016 ). Recent studies indicate that activating the Nrf2/ARE pathway has a hepato-protective effect ( Long et al, 2016 ).…”

Section: Discussionmentioning

confidence: 99%

Lactic Acid Bacteria With Antioxidant Activities Alleviating Oxidized Oil Induced Hepatic Injury in Mice

et al. 2018

View full text Add to dashboard Cite

In order to screening new Lactic acid bacteria (LAB) strains to alleviating liver injury induced by oxidized oil, we isolated and screened LAB from Chinese fermented foods. Lactobacillus plantarum AR113, Pediococcus pentosaceus AR243, and Lactobacillus plantarum AR501 showed higher scavenging activity of α, α-Diphenyl-β-Picrylhydrazyl (DPPH) free radical and hydrogen radical, stronger inhibition of lipid peroxidation, and better protective effect on yeast cells in vitro. In vivo, oral administration of L. plantarum AR501 improved the antioxidant status of injury mice induced by oxidized oil including decreasing lipid peroxidation, recovering activities of antioxidant enzymes. Meanwhile, the gene expression of Nuclear factor erythroid 2-related factor 2 (Nrf2) of L. plantarum AR501 group was markedly elevated, and several antioxidant genes such as glutathione S-transferase (GSTO1), heme oxygenase-1 (HO-1), Glutamate cysteine ligase (GCL), and NAD(P)H:quinone oxidoreductase-l (NQO1) were subsequently up regulated in mice liver. Therefore, L. plantarum AR501 could be considered as potential candidates for production of functional foods that can alleviate the oxidative damage induced by oxidized oil.

show abstract

“…In other studies, an active gypenoside compound known as phanoside, isolated from GP, exhibited a potent insulin-releasing activity [ 16 – 18 ]. Furthermore, using diabetic rat models, GP saponins induce hypoglycemia, hypolipidemia, and immunocompetence effects associated with antioxidant activities [ 19 , 20 ].…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antidiabetic Effects of the Traditional Medicinal PlantGynostemma pentaphyllumand the Possible Mechanisms of Insulin Release

Lokman

et al. 2015

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

Aims. To evaluate the antidiabetic effects of Gynostemma pentaphyllum (GP) in Goto-Kakizaki (GK) rat, an animal model of type 2 diabetes, and to investigate the mechanisms of insulin release. Methods. Oral glucose tolerance test was performed and plasma insulin levels were measured. Results. An oral treatment with GP (0.3 g/kg of body weight daily) for two weeks in GK rats improved glucose tolerance versus placebo group (P < 0.01). Plasma insulin levels were significantly increased in the GP-treated group. The insulin release from GP-treated GK rats was 1.9-fold higher as compared to the control group (P < 0.001). GP stimulated insulin release in isolated GK rat islets at high glucose. Opening of ATP-sensitive potassium (K-ATP) channels by diazoxide and inhibition of calcium channels by nifedipine significantly decreased insulin response to GP. Furthermore, the protein kinase A (PKA) inhibitor H89 decreased the insulin response to GP (P < 0.05). In addition, GP-induced insulin secretion was decreased after preincubation of GK islets with pertussis toxin to inhibit exocytotic Ge proteins (P < 0.05). Conclusion. The antidiabetic effect of GP is associated with the stimulation of insulin release from the islets. GP-induced insulin release is partly mediated via K-ATP and L-type Ca2+ channels, the PKA system and also dependent on pertussis toxin sensitive Ge-protein.

show abstract

Hypoglycemic effect of Gynostemma pentaphyllum saponins by enhancing the Nrf2 signaling pathway in STZ-inducing diabetic rats

Cited by 54 publications

References 33 publications

Effects of gypenosides on enteroendocrine L-cell function and GLP-1 secretion

Effects of gypenosides on enteroendocrine L-cell function and GLP-1 secretion

Lactic Acid Bacteria With Antioxidant Activities Alleviating Oxidized Oil Induced Hepatic Injury in Mice

Evaluation of Antidiabetic Effects of the Traditional Medicinal PlantGynostemma pentaphyllumand the Possible Mechanisms of Insulin Release

Contact Info

Product

Resources

About