Hypoglycemic Effects of a Polysaccharide from Tetrastigma hemsleyanum Diels & Gilg in Alloxan‐Induced Diabetic Mice

Abstract

Tetrastigma hemsleyanum Diels & Gilg, a well-known traditional Chinese medicine, possesses antitumor and anti-inflammatory activity, etc. However, the anti-diabetic effect has not been determined. In our present study, a water-soluble polysaccharide, named THP with molecular weight of 93 307 Da, was isolated from T. hemsleyanum by DEAE-52 ion-exchange and Sephadex G-100 chromatography. It contains rhamnose, arabinose, mannose, glucose, and galactose in the molar ratio of 0.07:0.14:0.38:0.21:0.31. Then anti-diabetic effects of THP were examined by treating alloxan-induced diabetic mice with different doses (100, 200, and 300 mg/kg) of THP orally. The results showed that THP could decrease the blood glucose, TC, TG, LDL-C levels, increase the body weight, HDL-C, insulin levels, and enhance the activities of antioxidant enzyme system in alloxan-induced diabetic mice. Furthermore, the histopathological examination of pancreas, liver, and kidney indicated that THP could protect and reverse β-cells in diabetic mice with low damage to liver and kidney, which suggests that THP may stimulate pancreatic release of insulin and can be an effectively potential candidate for diabetes mellitus.

Optimization of Degradation Conditions with PRG, a Polysaccharide from Phellinus ribis, by RSM and the Neuroprotective Activity in PC12 Cells Damaged by Aβ25–35

“…The monosaccharide composition of DPRG was analyzed using an Agilent HP6890 GC with a FID injector (Agilent Technologies, USA) equipped with a HP-5 capillary column (30 m × 0.25 mm) and a flame ionization detector. The temperature program was from 170 °C to 230 °C with a rate of 2 °C/min and at 230 °C for 5 min [39,40,41].…”

A comprehensive review on traditional uses, chemical compositions, pharmacology properties and toxicology of Tetrastigma hemsleyanum

“…Moreover, further study indicated RTP-3-1 was high purity polysaccharide with a molecular weight of 1244.2 kDa, and it is mainly composed of 4 kinds of monosaccharides: arabinose, galacturonic acid, galactose, and fructose, the proportion is 8.39%, 7.18%, 20.70%, and 63.70%, respectively. Ru et al, 2018a , Ru et al, 2018b ) extracted a polysaccharide THP from T. hemsleyanum , with the average molecular weight estimated as 93.307 kDa. The results of study on the composition of polysaccharide showed that it was mainly composed of rhamnose, arabinose, mannose, glucose, galactose with the molar ratio of 0.07:0.14:0.38:0.21:0.31.…”

“…Hua Shi Xuan Fei mixture (Approval number of Zhejiang medicine, Z20200026000), which is mainly composed of T. hemsleyanum , has been approved by Zhejiang Provincial Drug Administration for clinical treatment of COVID-19. Futhermore, the modern pharmacological studies had shown that T. hemsleyanum also had effects of anti-inflammatory ( Ji et al, 2019 ), antioxidant ( Hossain et al, 2011 ), antivirus ( Ding et al, 2019 ), antitumor ( Lin et al, 2014 ) antipyretic ( Yang and Wang, 2014 ), anti-hepatic injury ( Ma et al, 2012 ), immunomodulatory ( Xu et al, 2008 ), anti-bacterial ( Chen et al, 2019 ), hypoglycemic ( Ru et al, 2018a , Ru et al, 2018b ) etc. Numerous reports have demonstrated that the biological activities of T. hemsleyanum are attributed to its many chemical components ( Fu et al, 2019 ).…”

The Phytochemistry, Pharmacology, and Quality Control of Tetrastigma hemsleyanum Diels & Gilg in China: A Review

“…In addition to the above pharmacological activities, a recent study shows that a water-soluble polysaccharide with a molecular weight of 93307 Da, named THP, is extracted from TDG. THP has exhibited strong hypoglycemic activity in the alloxan induced mouse model, with its treatment being discovered to promote the activity of antioxidant enzymes (GSH-Px, SOD, and CAT) and reduce the content of malondialdehyde (MDA), while THP could restore the structure of pancreas and therefore affect insulin release, as it may be a natural candidate for the treatment of diabetes ( Ru et al., 2018 ). The research team obtained novel of polysaccharide (THDP-3) purified from the stems and leaves of TDG via anion exchange chromatography with a molecular weight of 77.98 kDa.…”