Hypoxia-driven changes in glycolytic and tricarboxylic acid cycle metabolites of two nodulated soybean genotypes

Borella, Júnior; Oliveira, Halley Caixeta; Oliveira, Denise dos Santos Colares de; Braga, Eugênia Jacira Bolacel; Oliveira, A.; Sodek, Ladaslav; Amarante, Luciano do

doi:10.1016/j.envexpbot.2016.10.007

Cited by 22 publications

(9 citation statements)

References 56 publications

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“…Altouhg genotypes Fundacep 53 RR and BRS Macota respond distinctly to hypoxia, tolerant and sensitive, respectively (Borella et al, 2014(Borella et al, , 2017, the antioxidative metabolism here studied was not correlated with tolerance mechanisms that differentiate genotypes and it is probably due to the short duration of flooding of the root system (Wang et al, 2009). However, tolerant species increase the activity of antioxidant enzymes to counteract the oxidative effects (Sairam et al, 2009;Simova-Stoilova et al, 2012).…”

Section: Discussionmentioning

confidence: 88%

“…The study was carried out at Federal University of Pelotas -Campus Capão do Leão (31º 52' 32" S and 52º 21' 24" W; altitude: 16 m), with two soybean [Glycine max (L.) Merril] genotypes, Fundacep 53 RR and BRS Macota, identified previously to respond distinctly to hypoxia (Borella et al, 2014(Borella et al, , 2017. The experiment comprised two groups, nodulated (N 2 -fixing) and nonnodulated (nitrate supplied) plants, grown in a greenhouse under natural light and temperature conditions.…”

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

“…Our previous work showed differences between Fundacep (tolerant) and Macota (susceptible) regarding N and C metabolism (Borella et al, 2017). Therefore, due to the lack of information regarding nitrate effects on the antioxidant status of waterlogged root system of soybean, this work investigated whether different N sources (nodulated and nitrate-supplied plants -nonnodulated) influence the antioxidant system of soybean plants by alleviating the oxidative effects caused by hypoxia.…”

Section: Introductionmentioning

confidence: 99%

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Nitrogen source influences the antioxidative system of soybean plants under hypoxia and re-oxygenation

Borella

Becker

Lima

et al. 2019

Sci. agric. (Piracicaba, Braz.)

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In this work, we compared nitrate-supplied plants (non-nodulated) with non-nitratesupplied plants (nodulated) under oxygen privation of root system (hypoxia) and re-oxygenation (post-hypoxia; recovery) in order to verify whether N sources affect the antioxidant system during oxidative stress caused by hypoxia and post-hypoxia conditions. Antioxidant enzymatic activities, ascorbate redox state, and reactive oxygen species (ROS) levels were analyzed in roots and leaves of two soybean genotypes, Fundacep 53 RR and BRS Macota at reproductive stage R2, during hypoxia and post-hypoxia in an experiment carried out in a hydroponic system. The antioxidant system was strongly induced in roots of nitrate-supplied plants of both genotypes, with high activity of superoxide dismutase, ascorbate peroxidase, catalase, glutathione reductase and guayacol peroxidase. It also increased reduced ascorbate and ascorbate redox state and decreased ROS production under hypoxia and recovery, while in leaves of nodulated and non-nodulated plants, a slight increase on antioxidant system was observed. Nitrate may benefit soybean plants under hypoxic conditions and subsequent re-oxygenation by inducing the antioxidant system mainly in roots to cope with ROS production and reduce oxidative damage.

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Section: Discussionmentioning

confidence: 88%

Section: Plant Materials and Growth Conditionsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Nitrogen source influences the antioxidative system of soybean plants under hypoxia and re-oxygenation

Borella

Becker

Lima

et al. 2019

Sci. agric. (Piracicaba, Braz.)

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“…After the plant is subjected to waterlogging stress, the oxygen diffusion rate in the soil is greatly reduced, oxygen supply is insufficient, tricarboxylic acid cycle is hindered, Anaerobic fermentation pathways such as glycolysis in which aerobic respiration enters anaerobic respiration (Vitou and Sodek. 2019;Borella et al 2017). LDH and ADH are mainly involved in anaerobic metabolism, under hypoxia environment, LDH is first activated to produce lactate, ADH is subsequently activated and then ethanol metabolism is started (Xu et al 2016).…”

Section: Discussionmentioning

confidence: 99%

Morpho-anatomical and physiological characteristics responses of a paried near-isogenic lines of waxy corn to waterlogging

Wang¹,

Lv²,

Wang³

et al. 2020

Emir J Food Agric

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Waterlogging stress is one of the most natural stress, which due to excessive rainfall or low-lying terrain, and limits crop growth and yield. Our objective was to study the morpho-anatomical and physiological characteristics of a paired near-isogenic lines of waxy corn under waterlogging stress. This experiment was implemented in pots with a paired of near-isogenic lines differing in waterlogging tolerance: Zz-R (waterlogging-resistant) and Zz-S (waterlogging-sensitive). Root morphology features, anaerobic respiratory enzyme activity, and tissue anatomical characteristics were measured at 0, 2, 4, 6 and 8d in the control and waterlogging conditions. The result indicated that waterlogging induced a decrease in the root length, volume and surface areas of the two waxy corn inbred lines, the total root length, root volume, and root surface area of the Zz-R showed less reduction than the Zz-S. Waterlogging stress influenced significantly by the parenchyma cells in cortex of root between Zz-R and Zz-S. Zz-R can also maintain a higher level of anaerobic respiratory enzyme activity under waterlogging stress. The different reaction between the paired near-isogenic lines may be responsible for higher tolerance of Zz-R than Zz-S. These results can provide a certain theoretical basis of the resistance evaluation and breeding to the resistant varieties.

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“…Besides, photorespiration is also reported to be an alternative sink drain of electrons in plants with C3 metabolism (Bräutigam and Gowik 2016;Posso et al 2018). Several authors have reported a rapid recovery of plants upon re-oxygenation, however, such experiments have employed other substrate than soil, such as vermiculite (Justino and Sodek 2013;Posso et al 2020), commercial substrate (Kissmann et al 2014), or even hydroponic system (Borella et al 2017). The characteristics of these substrates allow rapid and fast drainage and oxygen re-establishment in the root zone, increasing the recovery mechanism of plants.…”

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confidence: 99%

Short-term root flooding and recovery conditions—affected photosynthetic process of common bean plants

Posso

Borella

Reissig

et al. 2020

Theor. Exp. Plant Physiol.

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Photosynthetic process of common bean plants (Phaseolus vulgaris L.) was evaluated from short-term root flooding and recovery conditions. Common bean plants (BRS Expedito genotype) were grown in single plastic pots (1 L), containing soil as substrate. At the early reproductive stage (R1), distilled water was added up to 20 mm above the soil surface to flood the root system of plants for 1 day. The flooding was maintained by fitting a second pot without holes. After 1 day of flooding, the pots without holes were removed to drain water and recover the plants. Control plants were kept under normoxia. Chlorophyll a fluorescence transient, gas exchange, glycolate oxidase, antioxidative enzymes, reactive oxygen species (ROS) and lipid peroxidation were measured in leaves upon flooding (1 day) and recovery (1, 3 and 7 days) conditions. Root flooding (1 day) induced decrease (two-fold) in CO 2 assimilation rate and did not recover even after 7 days of normoxic conditions was re-established, besides limited transpiration rate and decreases in stomatal conductance. Moreover, the continuous light energy absorption by chlorophylls induced an increase in fluorescence and heat and impaired the connectivity between photosystems I and II, leading to ROS formation. The antioxidative enzyme system induced upon flooding and recovery conditions did not deal efficiently with ROS, which led to oxidative damage (lipid peroxidation) in leaves of common bean. Therefore, short-term root flooding impairs photosynthetic process recovery of common bean plants upon re-establishment of normoxic conditions. Keywords Antioxidative enzymes Á Oxidative damage Á Phaseolus vulgaris L. Á Photosynthesis Á Waterlogging 1 Introduction Climate changes are increasing flooding events since the 1950 0 s worldwide (Bailey-Serres et al. 2012; Limami et al. 2014; Pedersen et al. 2017), especially in the 28 million hectares of flood-prone located in Cerrado and lowlands in the South of Brazil (Jackson and Colmer 2005), due to increases in heavy rainfall (IPCC 2014). Currently, the lowland areas are mainly occupied with rice cropping system (Garcia et al. 2020), and food production by small farmers, such as common bean (Phaseolus vulgaris L.). Common bean

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Hypoxia-driven changes in glycolytic and tricarboxylic acid cycle metabolites of two nodulated soybean genotypes

Cited by 22 publications

References 56 publications

Nitrogen source influences the antioxidative system of soybean plants under hypoxia and re-oxygenation

Nitrogen source influences the antioxidative system of soybean plants under hypoxia and re-oxygenation

Morpho-anatomical and physiological characteristics responses of a paried near-isogenic lines of waxy corn to waterlogging

Short-term root flooding and recovery conditions—affected photosynthetic process of common bean plants

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