Hypoxia-induced redox alterations and their correlation with 99mTc-MIBI and 99mTc-HL-91 uptake in colon cancer cells

Abrantes, Ana Margarida; Serra, M. Elisa Silva; Gonçalves, Ana Cristina; Rio, Joana; Oliveiros, Bárbara; Laranjo, Mafalda; Rocha-Gonsalves, A.M.; Sarmento‐Ribeiro, Ana Bela; Botelho, Maria Filomena

doi:10.1016/j.nucmedbio.2009.11.001

Cited by 26 publications

(17 citation statements)

References 33 publications

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“…Regarding DHE, this compound crosses cell membrane and is converted by the superoxide radical to ethidium, a red fluorescent compound that intercalates into DNA. The quantified fluorescence of DCF or DHE is respectively proportional to the concentration of intracellular hydrogen peroxide or superoxide radical [20,21]. To perform this assay, 1x10…”

Section: Evaluation Of Hydrogen Peroxide and Superoxide Radical Exprementioning

confidence: 99%

Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

Mamede

Guerra

Laranjo

et al. 2016

Pathol. Oncol. Res.

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Section: Evaluation Of Hydrogen Peroxide and Superoxide Radical Exprementioning

confidence: 99%

Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

Mamede

Guerra

Laranjo

et al. 2016

Pathol. Oncol. Res.

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“…19,20 The cells were cultured in two different culture medium, HG and LG. The HG culture medium (with 25 mM of glucose) was the one who was designated by the supplier.…”

Section: [ 18 F]fdg Uptake Studiesmentioning

confidence: 99%

Fluorine-18 Fluorodeoxyglucose Uptake in Hepatocellular Carcinoma: Correlation with Glucose Transporters and p53 Expression

Brito

Abrantes

Ribeiro

et al. 2015

Journal of Clinical and Experimental Hepatology

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Introduction: Hepatocellular Carcinoma (HCC) is one of most lethal cancers worldwide. The prognosis is very poor and therapeutic options are limited. The aim of this study was to determine the correlation of the [ 18 F]FDG uptake profile of three HCC cell lines with p53 and glucose transporters (GLUTs) 1, 2, 3, 5 and 12 expression and with the glucose level present in the cell culture medium. Methods: Cell lines used are HepG2 (wp53), HuH7 (overexpress p53) and Hep3B2.1-7 (p53null). An immunocytochemical analysis was performed to evaluate p53 expression. Through uptake studies were analyzed the

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“…In this assay, 10 6 cells were incubated during 15 min in binding buffer with 1 µL of AV (Kit Immunotech, Marseille, France) and 5 µL of PI (Kit Immunotech, Marseille, France). Subsequently, cells were excited at a wavelength of 525 nm for AV and 640 nm for PI, collecting 10 4 events to assess the percentage of viable, early apoptotic, late apoptotic/necrotic, and necrotic cells (24).…”

Section: Cell Viabilitymentioning

confidence: 99%

Cytotoxicity of Ascorbic Acid in a Human Colorectal Adenocarcinoma Cell Line (WiDr): In Vitro and In Vivo Studies

Mamede

Pires

Abrantes

et al. 2012

Nutrition and Cancer

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Vitamin C, available in its reduced form (ascorbic acid; AA) and in its oxidized form (dehydroascorbic acid; DHA), may act in physiological conditions as an antioxidant or pro-oxidant. The aim of this study is to evaluate the cytotoxic effects of pharmacological doses of AA in a human colorectal adenocarcinoma cell line (WiDr) in vitro, through spectrophotometry, clonogenic assays and flow cytometry, and in vivo with xenotransplanted Balb/c nu/nu mice. The results show that the reduced form of vitamin C induces an anti-proliferative and cytotoxic effect in adenocarcinoma colorectal cells under study. The results obtained in vivo after treatment with AA showed a large reduction in the rate of tumor growth. Such understanding can guide decisions about which colorectal cancer patients might potentially benefit from vitamin C pharmacologic therapy.

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Hypoxia-induced redox alterations and their correlation with 99mTc-MIBI and 99mTc-HL-91 uptake in colon cancer cells

Cited by 26 publications

References 33 publications

Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

Oxidative Stress, DNA, Cell Cycle/Cell Cycle Associated Proteins and Multidrug Resistance Proteins: Targets of Human Amniotic Membrane in Hepatocellular Carcinoma

Fluorine-18 Fluorodeoxyglucose Uptake in Hepatocellular Carcinoma: Correlation with Glucose Transporters and p53 Expression

Cytotoxicity of Ascorbic Acid in a Human Colorectal Adenocarcinoma Cell Line (WiDr): In Vitro and In Vivo Studies

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