Hypoxia-Inducible miR-210 Regulates the Susceptibility of Tumor Cells to Lysis by Cytotoxic T Cells

Noman, Muhammad Zaeem; Buart, Stéphanie; Romero, Pedro; Ketari, Sami; Janji, Bassam; Mari, Bernard; Mami‐Chouaib, Fathia; Chouaı̈b, Salem

doi:10.1158/0008-5472.can-12-1383

Cited by 172 publications

(131 citation statements)

References 44 publications

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“…Moreover, some diagnostic miRNAs can be used in therapy. For instance, in previous studies, miR-155, which is downregulated in melanoma, was injected in melanoma cell lines to increase its expression (1,21,22). Similar procedures can be executed for miR-193b as a practical therapeutic option (1).…”

Section: Discussionmentioning

confidence: 99%

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Melanoma and Associated MicroRNAs

2016

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Context: Melanoma is an invasive type of skin cancer, with a rapidly increasing incidence. Therefore, new approaches are required to treat this aggressive cancer. MicroRNAs (miRNAs) are introduced as novel components in melanoma. This review study aimed to determine the relationship between melanoma and miRNAs. Evidence Acquisition: Prognostic, diagnostic, and therapeutic applications of miRNAs in skin cancer have been recently investigated from different aspects. Some of these studies on miRNAs were reviewed, and the mechanisms of some genetic modifications were determined in this study. Results: Since recommendations for miRNAs have increased in the past decades, and scientists have confirmed their great efficacy in multiple aspects of cancer treatment, different strategies have been developed considering their therapeutic effects. Therefore, further studies are required to confirm miRNA application in melanoma treatment. Conclusions: This review study included various investigations concerning miRNA traces as molecular rearrangements in melanoma. It was revealed that multiple miRNAs are efficient in molecular signaling and can be used as prognostic, diagnostic, and therapeutic biomarkers. Although the exact relationship between aberrant expression of miRNAs and cancers has been confirmed, further studies are required to introduce more thorough and accurate applications of miRNAs in melanoma.

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Section: Discussionmentioning

confidence: 99%

“…This gene modifies the cell surface of melanoma cells and affects cellular adherence; metastasis occurs due to discontinuity of cancerous cells. Therefore, miR-30b and miR-30 are accountable for metastasis in melanoma (1,21,22).…”

Section: Immunosuppressive Mirnasmentioning

confidence: 99%

Melanoma and Associated MicroRNAs

2016

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“…Increasing evidence, accumulated using microarray technology, has indicated a number of miRNAs that are differentially expressed in response to hypoxia. For example, miRNA-210, -155, -372/373 and -10b were shown to be upregulated, whereas miR-20b and -200b were found to be downregulated in response to hypoxia (5)(6)(7)(8). A recent study suggested that miRNA-150, a Hypoxia promotes C-X-C chemokine receptor type 4 expression through microRNA-150 in pancreatic cancer cells JIN-SHAN SUN 1* , XIAO-LAN ZHANG 2* , YU-JIE YANG 3* , ZHAN-GUO NIE 1 and YONG ZHANG hypoxia-sensitive miRNA, is involved in cancer metastasis via regulation of its target genes (9).…”

Section: Introductionmentioning

confidence: 99%

Hypoxia promotes C-X-C chemokine receptor type 4 expression through microRNA-150 in pancreatic cancer cells

et al. 2015

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Abstract. Hypoxia promotes pancreatic cancer progression by triggering cancer cell invasion. However, the mechanism underlying this process remains unclear, hindering the development of effective therapies. The present study aimed to delineate the molecular mechanisms underlying the prometastatic effect of hypoxia in pancreatic cancer cells. The expression of microRNA-150 (miRNA-150) was detected using reverse transcription-quantitative polymerase chain reaction in pancreatic cancer samples and in the hypoxia-induced CaPan2 human pancreatic cancer cell line. The target gene was identified using bioinformatics and a luciferase reporter assay. Inhibition of the expression of C-X-C chemokine receptor type 4 (CXCR4) by miRNA-150 was confirmed using transfection with miRNA-150 mimics. The prometastatic effect of hypoxia was detected using migration assays. The expression of miRNA-150 was shown to be downregulated in pancreatic cancer samples compared with that in normal pancreatic tissue samples. Furthermore, its expression was reduced in hypoxia-induced CaPan2 cells, compared with that in control cells. Bioinformatics and the results of the luciferase reporter assay, demonstrated that miRNA-150 inhibited the expression of CXCR4 by directly targeting the 3' untranslated region of CXCR4 mRNA. The results of the migration assay showed that hypoxia promotes cell migration and invasion. However, this prometastatic effect was reversed by transfection with miRNA-150 mimics. The present results suggest that hypoxia promotes pancreatic cancer migration by downregulating miRNA-150. IntroductionPancreatic cancer (PC) is among the most lethal malignancies, with a high incidence and rate of metastasis (1). At the time of diagnosis, the majority of patients are at an advanced stage of disease, with multi-organ metastasis, which indicates a poor prognosis for digestive system tumors (2). It is challenging to diagnose PC at an early stage, which results in a low 5-year survival rate; only 4% of patients diagnosed with pancreatic cancer survive after 5 years in China (3). Therefore, the identification of novel gene targets, which are differentially expressed in PC and functionally involved in the development of malignant phenotypes, is required in order to enable early diagnosis and the development of effective therapeutic strategies.Hypoxia is an important characteristic of solid tumors. As a tumor increases in size, it quickly outgrows its blood supply, leaving regions of the tumor, in which the oxygen concentration is significantly lower than that in normal tissues. In order to support tumor growth and proliferation within hypoxic environments, the expression of a number of genes is altered, and changes in metabolism also occur (4). Recently, a novel class of endogenous small non-coding regulatory RNAs, termed microRNAs (miRNAs), has received increasing attention. These small molecules exert their regulatory effects by base pairing with partially complementary messenger RNAs (mRNAs). They act via one of two mechanisms: Degradatio...

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“…It has been demonstrated that miR-210 plays an important role in regulation of cell growth, angiogenesis, invasion and apoptosis in different human tumour models [5,11]. MiR-210 expression is frequently up-regulated in a variety of solid tumours, including breast cancer [12], non-small cell lung cancer [29], head and neck cancer [8], pancreatic cancer [4], oral tumours [31], hepatocellular cancer (HCC) [42], adrenocortical carcinoma (ACC) [23], glioblastoma [26], colon cancer [24], ovarian cancer [9], malignant melanoma [22] and renal cell cancer [27]. The expression of miR-210 is found to be down-regulated in human esophageal squamous cell carcinoma (ESCC) tissues and derived cell lines [34].…”

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confidence: 99%