Hypoxia Promotes Mineralization of Human Dental Pulp Cells

Li, Lifen; Zhu, Ye; Jiang, Long; Peng, Wei; Ritchie, Helena H.

doi:10.1016/j.joen.2011.02.028

Cited by 58 publications

(43 citation statements)

References 26 publications

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“…A various factors increased the hDPCs viability. For instance, 5 % O 2 hypoxia increased the hDPCs viability [17]. In other study, rhFGF2 also increased the hDPC viability [15].…”

Section: Discussionmentioning

confidence: 62%

Expression, Purification, and Characterization of a Dentin Phosphoprotein Produced by Escherichia coli, and Its Odontoblastic Differentiation Effects on Human Dental Pulp Cells

Yun¹,

Jeon

Kang

et al. 2012

Protein J

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To investigate the functions of recombinant human dentin phosphoprotein (rhDPP), we examined cell adhesion, viability and the odontoblastic differentiation activity of human dental pulp cells (hDPCs). Firstly, rhDPP was constructed using pBAD-HisA plasmid in Escherichia coli. Cell adhesion and viability of hDPCs by rhDPP was examined using a crystal violet assay and a MTT assay, ALP, mineralization activity and odontoblastic differentiation-related mRNA levels of hDPCs were measured to elucidate the odontoblastic differentiation effect of rhDPP on hDPCs. Initially, rhDPP significantly and dose-dependently increased hDPCs adhesion versus the untreated control (p < 0.05). Cell viability was also significantly increased by rhDPP at 5 days (p < 0.001). Furthermore, the odontoblastic differentiation effect of rhDPP was verified by measuring ALP activity, mineralization activity and the mRNA levels of odontoblastic differentiation markers. Taken together, rhDPP is expected to play an important role on hDPCs, thereby suggesting its potential use for tooth repair and regeneration.

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“…A various factors increased the hDPCs viability. For instance, 5 % O 2 hypoxia increased the hDPCs viability [17]. In other study, rhFGF2 also increased the hDPC viability [15].…”

Section: Discussionmentioning

confidence: 62%

Expression, Purification, and Characterization of a Dentin Phosphoprotein Produced by Escherichia coli, and Its Odontoblastic Differentiation Effects on Human Dental Pulp Cells

Yun¹,

Jeon

Kang

et al. 2012

Protein J

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“…These findings suggest that 2% O 2 hypoxic treatment may promote differentiation potential of PDLCs and DPCs. Even though the mechanism by which hypoxia influences the differentiation capacity of PDLCs and DPCs is not clearly understood, it could be speculated that HIF-1 α is activated in PDLCs and DPCs after exposure to hypoxia and then induces cell signalling pathways such as Wnt, Notch, and Sonic hedgehog (Shh), which help maintain the cell stemness and enhance the differentiation capacity [32, 33]. Further studies need to be conducted to clarify the molecular mechanisms behind these hypoxia-related phenomena.…”

Section: Discussionmentioning

confidence: 99%

The Effect of Hypoxia on the Stemness and Differentiation Capacity of PDLC and DPC

Zhou

Wei

Xiao

2014

BioMed Research International

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Introduction. Stem cells are regularly cultured under normoxic conditions. However, the physiological oxygen tension in the stem cell niche is known to be as low as 1-2% oxygen, suggesting that hypoxia has a distinct impact on stem cell maintenance. Periodontal ligament cells (PDLCs) and dental pulp cells (DPCs) are attractive candidates in dental tissue regeneration. It is of great interest to know whether hypoxia plays a role in maintaining the stemness and differentiation capacity of PDLCs and DPCs. Methods. PDLCs and DPCs were cultured either in normoxia (20% O2) or hypoxia (2% O2). Cell viability assays were performed and the expressions of pluripotency markers (Oct-4, Sox2, and c-Myc) were detected by qRT-PCR and western blotting. Mineralization, glycosaminoglycan (GAG) deposition, and lipid droplets formation were assessed by Alizarin red S, Safranin O, and Oil red O staining, respectively. Results. Hypoxia did not show negative effects on the proliferation of PDLCs and DPCs. The pluripotency markers and differentiation potentials of PDLCs and DPCs significantly increased in response to hypoxic environment. Conclusions. Our findings suggest that hypoxia plays an important role in maintaining the stemness and differentiation capacity of PDLCs and DPCs.

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“…Hypoxia is another factor postulated to encourage mineralization [40]. Hypoxia could be more prominent in the centres of large aggregates, thus explaining the relationship between aggregate size and mineralization.…”

Section: Discussionmentioning

confidence: 99%

A facile in vitro model to study rapid mineralization in bone tissues

Deegan

Aydin

et al. 2014

BioMed Eng OnLine

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BackgroundMineralization in bone tissue involves stepwise cell-cell and cell-ECM interaction. Regulation of osteoblast culture microenvironments can tailor osteoblast proliferation and mineralization rate, and the quality and/or quantity of the final calcified tissue. An in vitro model to investigate the influencing factors is highly required.MethodsWe developed a facile in vitro model in which an osteoblast cell line and aggregate culture (through the modification of culture well surfaces) were used to mimic intramembranous bone mineralization. The effect of culture environments including culture duration (up to 72 hours for rapid mineralization study) and aggregates size (monolayer culture as control) on mineralization rate and mineral quantity/quality were examined by osteogenic gene expression (PCR) and mineral markers (histological staining, SEM-EDX and micro-CT).ResultsTwo size aggregates (on average, large aggregates were 745 μm and small 79 μm) were obtained by the facile technique with high yield. Cells in aggregate culture generated visible and quantifiable mineralized matrix within 24 hours, whereas cells in monolayer failed to do so by 72 hours. The gene expression of important ECM molecules for bone formation including collagen type I, alkaline phosphatase, osteopontin and osteocalcin, varied temporally, differed between monolayer and aggregate cultures, and depended on aggregate size. Monolayer specimens stayed in a proliferation phase for the first 24 hours, and remained in matrix synthesis up to 72 hours; whereas the small aggregates were in the maturation phase for the first 24 and 48 hour cultures and then jumped to a mineralization phase at 72 hours. Large aggregates were in a mineralization phase at all these three time points and produced 36% larger bone nodules with a higher calcium content than those in the small aggregates after just 72 hours in culture.ConclusionsThis study confirms that aggregate culture is sufficient to induce rapid mineralization and that aggregate size determines the mineralization rate. Mineral content depended on aggregate size and culture duration. Thus, our culture system may provide a good model to study regulation factors at different development phases of the osteoblastic lineage.Electronic supplementary materialThe online version of this article (doi:10.1186/1475-925X-13-136) contains supplementary material, which is available to authorized users.

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Hypoxia Promotes Mineralization of Human Dental Pulp Cells

Cited by 58 publications

References 26 publications

Expression, Purification, and Characterization of a Dentin Phosphoprotein Produced by Escherichia coli, and Its Odontoblastic Differentiation Effects on Human Dental Pulp Cells

Expression, Purification, and Characterization of a Dentin Phosphoprotein Produced by Escherichia coli, and Its Odontoblastic Differentiation Effects on Human Dental Pulp Cells

The Effect of Hypoxia on the Stemness and Differentiation Capacity of PDLC and DPC

A facile in vitro model to study rapid mineralization in bone tissues

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