Hyunsoonleella jejuensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seawater

Abstract

Hyunsoonleella jejuensis gen. nov., sp. nov., a novel member of the family Flavobacteriaceae isolated from seawater , was isolated from a seawater sample collected on the coastline of Jeju Island, South Korea. The strain was strictly aerobic, non-flagellated, non-gliding and oxidase-and catalase-positive. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CNU004 T belongs to a distinct lineage in the family Flavobacteriaceae. Strain CNU004 T exhibited levels of 16S rRNA gene sequence similarity of 93.8-93.9 % to its nearest phylogenetic neighbours, members of the genera Gaetbulibacter, Yeosuana and Algibacter. The new isolate required sea salts or artificial seawater for growth. The optimum ranges of temperature and pH for growth were 30-35 6C and pH 7.0-8.0. The DNA G+C content of strain CNU004 T was 37.7 mol%. The major fatty acids were iso-C 15 : 0 , iso-C 15 : 1 G, iso-C 17 : 0 3-OH and iso-C 15 : 0 3-OH. Menaquinone-6 was the major respiratory quinone. Zeaxanthin was the major carotenoid pigment produced, and flexirubin-type pigments were not produced. Strain CNU004 T was able to degrade starch and agar. Based on its phenotypic and genotypic characteristics and on the phylogenetic evidence presented, strain CNU004 T is considered to represent a novel species of a new genus in the family Flavobacteriaceae, for which the name Hyunsoonleella jejuensis gen. nov., sp. nov. is proposed. The type strain of Hyunsoonleella jejuensis sp. nov. is CNU004The family Flavobacteriaceae (Reichenbach, 1989;Bernardet et al., 2002;Bernardet & Nakagawa, 2006) is one of the major phylogenetic lineages within the phylum Bacteroidetes (Garrity & Holt, 2001). At the time of writing, the family comprises more than 50 recognized genera. Many members of this family were isolated from various marine ecosystems and Antarctic habitats (Bowman et al., 1997(Bowman et al., , 1998Bowman, 2000;Bowman & Nichols, 2002Cho & Giovannoni, 2004;Nedashkovskaya et al., 2004;Nichols et al., 2005;Pinhassi et al., 2006). Members of most genera in the family Flavobacteriaceae produce light-to bright-yellow pigments. These pigments may belong to the carotenoid and/or the flexirubin types depending on the genus (Bernardet et al., 2002 et al., 2007). In the present study, we report the isolation, identification and characterization of a novel zeaxanthinproducing marine bacterium isolated from seawater. Strain CNU004T was isolated from a seawater sample collected along the coastline of Jeju Island, Republic of Korea. Serially diluted seawater samples were spread on marine agar 2216 (MA; Difco) and incubated at 30 u C for 3These authors contributed equally to this work.

Arenitalea lutea gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from intertidal sand

“…Aquimarina addita JC2680 T (HM475137) with the one formed by the genera Hyunsoonleella (Yoon et al, 2010) and Jejuia (Lee et al, 2009), within a cluster encompassing the genera Hyunsoonleella, Jejuia and Marinivirga and two species of the genus Algibacter (Park et al, 2013b). Topologies of the maximum-parsimony and maximum-likelihood trees were nearly identical to the one shown by the neighbour-joining tree (Figs S1 and S2 available in IJSEM Online).…”

“…n. Arenitalea a sand rod, referring to a bacterium isolated from intertidal sand). Yoon et al (2010). DData from Lee et al (2009).…”

Jejuia marina nov., isolated from gravel adjacent to Geommeolle beach on Udo Island, South Korea

“…Fatty acids comprising less than 1.0 % of the total in stains are not shown. All data are from this study tr, amount are less than 1.0 % of the total; -, not detected a Not previously reported as present byYoon et al (2010) …”

Hyunsoonleella rubra sp. nov., isolated from coastal sediment

“…In recent years, many new taxa have been described in the family Flavobacteriaceae; the new genus Hyunsoonleella in the family was proposed by Yoon et al [1]. At the time of writing, the genus Hyunsoonleella comprised two species: Hyunsoonleella jejuensis [1] and Hyunsoonleella pacifica [2].…”

“…At the time of writing, the genus Hyunsoonleella comprised two species: Hyunsoonleella jejuensis [1] and Hyunsoonleella pacifica [2]. Bacterial cells of the genus Hyunsoonleella are rod shaped, Gram-stain-negative and non-gliding.…”