Caesalpinia sappan Induces Cell Death by Increasing the Expression of p53 and p21WAF1/CIP1 in Head and Neck Cancer Cells

Kim, Eun-Cheol; Hwang, Young-Soo; Lee, Hwa-Jeong; Lee, Sun-Kyung; Park, Myung-Hee; Jeon, Byung-Hun; Jeon, Chang‐Jin; Lee, Suk-Keun; Yu, Hyeon-Hee; You, Yong‐Ouk

doi:10.1142/s0192415x05003016

Cited by 39 publications

(25 citation statements)

References 12 publications

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“…Isolated compounds from the heartwood inhibited the growth of several cancer cell lines (Kim et al, 2005;Lee et al, 2011b;Liang et al, 2013). However, this is the first report on the cytotoxic activity of the constituents of Vietnamese C. sappan seeds in HL-60 and some other cell lines.…”

Section: Resultsmentioning

confidence: 70%

Cytotoxic constituents from the seeds of VietnameseCaesalpinia sappan

Tran

Nguyen

et al. 2015

Pharmaceutical Biology

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Context: Caesalpinia sappan Linn. (Leguminosae) has been used in folk medicines for the treatment of many diseases. The heartwood of this plant contains various phenolic components with interesting biological applications; however, the chemical and biological potentials of the seed of this plant have not been fully explored.Objective: This study identified the cytotoxic activity of compounds from the seeds of C. sappan. Materials and methods: The methanol extract of the seed of C. sappan was suspended in H 2 O and then partitioned with CH 2 Cl 2 , EtOAc, and n-BuOH, successively. Diterpenoid compounds were isolated from the CH 2 Cl 2 -soluble fraction by silica gel column chromatography methods using organic solvents. The compound structures were determined by detailed analysis of NMR and MS spectral data. Cytotoxic activity was measured using a modified MTT assay against HL-60, HeLa, MCF-7, and LLC cancer cells. The activation of caspase-3 enzyme and western blotting assay were performed to confirm inhibitory mechanism of active compound. Results: Five cassane-type diterpenoids were isolated and identified as phanginin I (1), phaginin A (2), phanginin D (3), phanginin H (4), and phanginin J (5). Compounds 1-4 showed effective inhibition against HL-60 cells with the IC 50 values of 16.4 ± 1.5, 19.2 ± 2.0, 11.7 ± 1.6, and 22.5 ± 5.1 mM. Compounds 1-3 exhibited cytotoxic activity against HeLa cells with the IC 50 values of 28.1 ± 3.6, 37.2 ± 3.4, and 22.7 ± 2.8 mM. Treatment of HL-60 cell lines with various concentrations of 3 (0-30 mM) resulted in the growth inhibition and induction of apoptosis.Conclusion: These findings demonstrate that compound 3 (phanginin D) is one of the main active components of the seed of C. sappan activating caspases-3 which contribute to apoptotic cell death.

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Section: Resultsmentioning

confidence: 70%

Cytotoxic constituents from the seeds of VietnameseCaesalpinia sappan

Tran

Nguyen

et al. 2015

Pharmaceutical Biology

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“…50 It was also evidenced by Kim et al that chloroform extract of Caesalpinia sappan increased the expression of p53 in head and neck cancer cells in vitro. 52 From our study, it is evident that vanadium mediated induction of p53 protein is causing inhibition of cell proliferation and a possible induction of apoptosis.…”

Section: Discussionmentioning

confidence: 68%

Suppression of cell proliferation, induction of apoptosis and cell cycle arrest: Chemopreventive activity of vanadium in vivo and in vitro

Ray

Ghosh

Rana

et al. 2006

Intl Journal of Cancer

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In the present study, the authors evaluated the anticancer mechanism of vanadium, a dietary micronutrient and an important pharmacological agent, on a defined model of chemically induced rat mammary carcinogenesis in vivo and on human breast cancer cell line MCF7 in vitro. Female Sprague-Dawley rats were treated with 7,12-dimethylbenz(a)anthracene (0.5 mg/100 g body weight) by a single tail vein injection in an oil emulsion to induce mammary preneoplasia. Vanadium (ammonium monovanadate) at a concentration of 0.5 ppm (4.27 lmol/l) was supplemented in drinking water and given ad libitum to the experimental groups for 24 weeks. Histological finding showed substantial repair of hyperplastic lesions. There was a significant reduction in incidence, multiplicity (34%, p < 0.01), size of palpable mammary tumors and delay in mean latency period of tumor appearance. Immunohistochemical analysis in vivo indicated a decrease in cell proliferation (24.68% p < 0.05) and an increase among the TUNEL-positive apoptotic cells along with strong expressions of p53 and Bax, and downregulation of Bcl2 proteins in the mammary tissue of vanadium-treated animals. Further, MCF7 cells were cultured in minimal essential medium and were treated with 100, 175 and 250 lM of vanadium (ammonium monovanadate) for 36 hr. Exposure of MCF7 cells to vanadium led to induction of apoptosis in a dose-dependent manner. It was found further that vanadium treatment brought about a prominent cell cycle arrest and chromosomal condensation, leading to apoptosis (42.62%, p < 0.05). Results of both the in vivo and in vitro study demonstrate that vanadium has the potential to be developed into an anti-breast cancer drug in the near future. ' 2006 Wiley-Liss, Inc.

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“…Upregulation of p21 might occurred through p53 independent pathway leading to inhibition of cell cycle . Extract of C. sappan also showed anticancer activity on HNSCC4 and HNSCC31 cells (head and neck cancer cells) upregulation of p21WAF1/CIP1 (Kim, et al, 2005). SEE proposed to inhibit T47D cell viability through antiestrogenic activity, and inhibition cell cycle, but the mechanism need to be explored further.…”

Section: Discussionmentioning

confidence: 99%

“…Park, et al (2002) and Wicaksono, et al (2008) reported that secang has cytotoxic effects on Hep G2 and Hep 3B cancer cells. Extract of C.sappan heartwood performed anticancer activity on HNSCC4 and HNSCC31 head and neck cancer cells (Kim, et al, 2005). Ethanolic extract of C. sappan also showed potent radical scavenging activity of reactive oxygen species (ROS) and reactive nitrogen species (RNS) .…”

Section: Introductionmentioning

confidence: 99%

Heartwood of Secang (CAESALPINIA SAPPAN L.) Ethanolic Extract Show Selective Cytotoxic Activities on T47D and Widr Cells But Not on Hela Cells

Rivanti

Shabrina

Nurzijah

et al. 2017

IJCC

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The present study investigate the selectivity of heartwood of secang ethanolic extract (SEE) on T47D breast cancer cells, WiDr colon cancer cells, and HeLa cervical cancer cells, compared to Vero normal epithelial cells. The cytotoxic effect was evaluated by using MTT assay with 24-hour treatment to get IC50 values. Selectivity was evaluated by using selectivity index (SI). SEE had a potent cytotoxic activity on T47D and WiDr cancer cells (IC50<100 µg/mL). IC50 value of HeLa cancer cells was observed on moderate cytotoxic (1003), while in HeLa cells is not selective (SI<3). This result indicating its potential of Caesalpinia sappan as a chemopreventive agent in cancer therapy.

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Caesalpinia sappan Induces Cell Death by Increasing the Expression of p53 and p21^WAF1/CIP1 in Head and Neck Cancer Cells

Cited by 39 publications

References 12 publications

Cytotoxic constituents from the seeds of VietnameseCaesalpinia sappan

Cytotoxic constituents from the seeds of VietnameseCaesalpinia sappan

Suppression of cell proliferation, induction of apoptosis and cell cycle arrest: Chemopreventive activity of vanadium in vivo and in vitro

Heartwood of Secang (CAESALPINIA SAPPAN L.) Ethanolic Extract Show Selective Cytotoxic Activities on T47D and Widr Cells But Not on Hela Cells

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