Camellia nitidissima Chi leaf as pancreatic lipase inhibitors: Inhibition potentials and mechanism

Abstract

In this study, Camellia nitidissima Chi leaf extract was investigated for its compounds and pancreatic lipase inhibitory potentials. The interaction was determined using ultraviolet (UV) spectroscopy, circular dichroism (CD), fluorescence spectroscopy (FS), and molecular docking to understand the inhibiton, kinetic, and conformation of extraction–pancreatic lipase complex. C. nitidissima Chi leaf extraction inhibited the pancreatic lipase activity in a dose‐dependent manner at the concentration of 1–12 mg/ml. The Lineweaver–Burk plots indicated that the inhibition on pancreatic lipase by extraction was noncompetitive. In addition, the decrease in α‐helix contents, increase in β‐sheet and β‐turn, and decrease in fluorescence intensity after extraction treatment indicated that the conformation of pancreatic lipase was changed. This work revealed that C. nitidissima Chi leaf extraction played a significant role in inhibiting pancreatic lipase activity and brought out a solution of delay fat accumulation. Practical applications This study reports the components in the extract of C. nitidissima Chi leaf and its inhibitory effect and mechanism of pancreatic lipase. C. nitidissima Chi leaf is a good source of bioactive components, including multiflorin B, kaempferol‐3‐O‐rutinoside, vicenin‐2, apigenin‐6‐C‐pentosyl‐8‐C‐hexosyl, vitexin, kaempferol, and other ingredients. It can inhibit pancreatic lipase and be used to control obesity and treat hyperlipidemia. This study also revealed the structure changes of C. nitidissima Chi leaf extract on pancreatic lipase, and further revealed the inhibitory mechanism of C. nitidissima Chi leaf extract on lipase, which provides a theoretical basis for C. nitidissima Chi leaf as a lipase inhibitor.

Active fractions of golden-flowered tea (Camellia nitidissima Chi) inhibit epidermal growth factor receptor mutated non-small cell lung cancer via multiple pathways and targets in vitro and in vivo

“…Interestingly, the slight change in body weight of nude mice with increasing drug doses. However, physiological and biochemical results and H&E sections of the liver and kidney confirmed the absence of significant drug toxicity, suggesting CLS may play a role in lipid-lowering ( Figure 8B and Supplementary Figure 2 ) ( 35 ). Also, we performed WB validation in tissue samples of the proteins screened in the previous experiments, with results generally consistent with cell samples ( Figures 8H,I ).…”

“…In addition, H&E results and physiological and biochemical results of the liver and kidney showed no significant hepatic or renal toxicity with CLS treatment ( Supplementary Figure 2 ). This result might be based on the ability of CNC to inhibit lipase activity ( 34 , 35 ). Compared with untreated mice in the control group, the CLS 100 mg/kg showed a tumor inhibition effect since day 9 ( Figure 8C ) while CLS 200 mg/kg and 400 mg/kg treated group significantly suppressed tumor growth starting at day 5 ( Figures 8C,D ).…”

Inquiry lipaseoring the mechanism of pancreatic lipase inhibition by isovitexin based on multispectral method and enzyme inhibition assay

“…The determination of reaction rates was conducted when P-NPP of 0.5, 1, 1.5 and 2 mM reacts with substrate. The Lineweaver-Burk double inversion method was utilized to determine the inhibition kinetics of sample PL [31].…”

Rapid Screening of Lipase Inhibitors from Ophiopogonis Radix Using High-Performance Thin Layer Chromatography by Two Step Gradient Elution Combined with Bioautographic Method

“…However, orlistat, the only lipase inhibitor approved clinically, has several adverse effects such as severe liver and kidney injury [3,4]. In recent years, more and more medicinal herbs have been reported to show inhibitory activities against pancreatic lipase, such as Morus alba [5], Atractylodes lancea [6], Forsythia suspensa [7], and Camellia nitidissima [8]. Therefore, discovery of lipase inhibitors from herbal medicines may provide a potential alternative for the treatment of obesity.…”