<i>Candida albicans</i><i>PHO81</i> is required for the inhibition of hyphal development by farnesoic acid

Chung, Soon‐Chun; Kim, Tae Im; Ahn, Chan‐Hong; Shin, Jongheon; Oh, Ki‐Bong

doi:10.1016/j.febslet.2010.10.026

Cited by 13 publications

(19 citation statements)

References 41 publications

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“…Farnesol regulates the expression of several genes and induces TUP1, a transcriptional cofactor repressing filamentation [27], while repressing CPH1 and HST7 expression, which are both activators of the morphological switch [28]. The oxidized form of farnesol, farnesoic acid, has also been reported to inhibit hyphal growth by acting via PHO81 [29]. However, morphological inhibition is stronger with farnesol, although farnesoic acid is less toxic at high concentration [30], it displays only 3% of farnesols QSM activity [31].…”

Section: Alcohols: Quorum Sensingmentioning

confidence: 99%

Communication in Fungi

Cottier

Mühlschlegel

2012

International Journal of Microbiology

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We will discuss fungal communication in the context of fundamental biological functions including mating, growth, morphogenesis, and the regulation of fungal virulence determinants. We will address intraspecies but also interkingdom signaling by systematically discussing the sender of the message, the molecular message, and receiver. Analyzing communication shows the close coevolution of fungi with organisms present in their environment giving insights into multispecies communication. A better understanding of the molecular mechanisms underlying microbial communication will promote our understanding of the “fungal communicome.”

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Section: Alcohols: Quorum Sensingmentioning

confidence: 99%

Communication in Fungi

Cottier

Mühlschlegel

2012

International Journal of Microbiology

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“…Literature data described that individual pleomorphic forms of Candida albicans provide critical functions required for the pathogenic lifestyle (Cullen and Sprague, 2000; Yang et al , 2009; Chung et al , 2010; Ness et al , 2010; Noble et al , 2010). Although the distinguishing criteria between various morphotypes are still a matter of debate, some of them have been established based upon transmission electron microscopy (TEM) by Müller and Melchinger (2006).…”

Section: Introductionmentioning

confidence: 99%

Candida albicans morphologies revealed by scanning electron microscopy analysis

Staniszewska¹,

Bondaryk²,

Swoboda-Kopeć³

et al. 2013

Braz. J. Microbiol.

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Scanning electron microscope (SEM) observations were used to analyze particular morphologies of Candida albicans clinical isolate (strain 82) and mutants defective in hyphae-promoting genes EFG1 (strain HLC52) and/or CPH1 (strains HLC54 and Can16). Transcription factors Efg1 and Cph1 play role in regulating filamentation and adhesion of C. albicans’ morphologies. Comparative analysis of such mutants and clinical isolate showed that Efg1 is required for human serum-induced cell growth and morphological switching. In the study, distinct differences between ultrastructural patterns of clinical strain’s and null mutants’ morphologies were observed (spherical vs tube-like blastoconidia, or solid and fragile constricted septa vs only the latter observed in strains with EFG1 deleted). In addition, wild type strain displayed smooth colonies of cells in comparison to mutants which exhibited wrinkled phenotype. It was observed that blastoconidia of clinical strain exhibited either polarly or randomly located budding. Contrariwise, morphotypes of mutants showed either multiple polar budding or a centrally located single bud scar (mother-daughter cell junction) distinguishing tube-like yeast/pseudohyphal growth (the length-to-width ratios larger than 1.5). In their planktonic form of growth, blastoconidia of clinical bloodstream isolate formed constitutively true hyphae under undiluted human serum inducing conditions. It was found that true hyphae are essential elements for developing structural integrity of conglomerate, as mutants displaying defects in their flocculation and conglomerate-forming abilities in serum. While filamentation is an important virulence trait in C. albicans the true hyphae are the morphologies which may be expected to play a role in bloodstream infections.

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“…However, it possessed only 3% of farnesol's inhibitory activity when germ tube formation was induced in N-acetylglucosamine-containing medium (126). PHO81, which encodes a phosphatase, was recently shown to be required for farnesoic acid to inhibit the Y-H transition (26,27). Additionally, PHO81 was proposed to be a negative regulator of Ras1p activity, as it appears to act upstream of Ras1p signaling (26).…”

Section: Bacterial and Fungal Autoregulatory Moleculesmentioning

confidence: 99%

“…PHO81, which encodes a phosphatase, was recently shown to be required for farnesoic acid to inhibit the Y-H transition (26,27). Additionally, PHO81 was proposed to be a negative regulator of Ras1p activity, as it appears to act upstream of Ras1p signaling (26). In contrast to these hypha-inhibiting molecules, tyrosol was described as a QS molecule that stimulated germ tube formation (23) and promoted hyphal development in the early stages of biofilm formation (4).…”

Section: Bacterial and Fungal Autoregulatory Moleculesmentioning

confidence: 99%

Modulation of Morphogenesis in Candida albicans by Various Small Molecules

2011

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The pathogenic yeast Candida albicans, a member of the mucosal microbiota, is responsible for a large spectrum of infections, ranging from benign thrush and vulvovaginitis in both healthy and immunocompromised individuals to severe, life-threatening infections in immunocompromised patients. A striking feature of C. albicans is its ability to grow as budding yeast and as filamentous forms, including hyphae and pseudohyphae. The yeast-to-hypha transition contributes to the overall virulence of C. albicans and may even constitute a target for the development of antifungal drugs. Indeed, impairing morphogenesis in C. albicans has been shown to be a means to treat candidiasis. Additionally, a large number of small molecules such as farnesol, fatty acids, rapamycin, geldanamycin, histone deacetylase inhibitors, and cell cycle inhibitors have been reported to modulate the yeast-to-hypha transition in C. albicans. In this minireview, we take a look at molecules that modulate morphogenesis in this pathogenic yeast. When possible, we address experimental findings regarding their mechanisms of action and their therapeutic potential. We discuss whether or not modulating morphogenesis constitutes a strategy to treat Candida infections.

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Candida albicansPHO81 is required for the inhibition of hyphal development by farnesoic acid

Cited by 13 publications

References 41 publications

Communication in Fungi

Communication in Fungi

Candida albicans morphologies revealed by scanning electron microscopy analysis

Modulation of Morphogenesis in Candida albicans by Various Small Molecules

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