In Silico and In Vitro Screening of Ethanolic Extract of Fruits of Withania Coagulans against Diabetes

Meeran, Sahina Begum; Subburaya, Umamaheswari; Narasimhan, Gopinathan

doi:10.5958/0974-360x.2020.00120.1

Cited by 15 publications

(7 citation statements)

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“…The conformance of glucose uptake with the predictions of the carrier hypothesis adds strong support to the general significance of this mechanism in sugar uptake in yeast. The recent evidence that carrier reactions are also involved in the sugar efflux reactions in Escherichia coli 12 and in sugar transport in Tetrahymena supports the conclusion that this mechanism is as fundamental in microorganisms as in higher forms 9 . Therefore the basic aim of this study is to confirm antidiabetic action of Withania coagulans by using the in vitro method of glucose uptake by yeast cells using fruit extract of the sample.…”

Section: Yeast Cell For Glucose Uptake Determinationmentioning

confidence: 67%

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2023

IJPSR

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The present study estimated the antidiabetic activity of methanolic extract of plant fruits of plant Withania coagulans L. Dunal. Withania coagulans (family Solanaceae) is commonly known as "Paneer ke phool" in Hindi and "Indian cheese maker" or "Vegetable rennet" in English, distributed in drier parts of India. The primary aim of this study was to confirm antidiabetic action of Withania coagulans by using the invitro method of glucose uptake by yeast cells using methanolic fruit extract of the sample. It is reported that the Withanolides are responsible for its anti-hyperglycemic activity, which has been standardized by the TLC method using 50% Sulphuric acid and dragendroff's reagents as a spraying agent in this study. The current research found that Withania coagulans methanolic fruit extract improved glucose absorption in yeast cells by 0-90% at various glucose concentrations (5, 10, and 25 mM). Metformin was used as a standard reference. At all glucose concentrations, the highest concentration of WCME (5 mg/ml WCME) demonstrated maximum activity and showed the greatest increase (81.83 percent) in the presence of 25 mM glucose. In conclusion, this report suggests that the increase of glucose transport across the cell membrane might be the anti-hyperglycaemic mechanism of action of Withania coagulans fruits. INTRODUCTION:Traditional medicine is "the knowledge, skills, and practices based on the theories, beliefs, and experiences indigenous to different cultures, used in the maintenance of health and in the prevention, diagnosis, improvement or treatment of physical and mental illness. The vast use of Traditional medicine may be due to many reasons like high cost and long-time taken to develop the new drug, toxicity, and non-renewable source of basic raw materials.

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Section: Yeast Cell For Glucose Uptake Determinationmentioning

confidence: 67%

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2023

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“…Though plenty of work has been done to study its antidiabetic activity, not much work is done to evaluate its action on the CNS. [18][19][20] In 1977 Budhiraja et al reported CNS depressant effect of this plant. 21 Thereafter this plant was not much studied for the CNS activity.…”

Section: Introductionmentioning

confidence: 98%

The motor coordination activity of alcoholic extract of Withania coagulans fruits in Swiss albino mice by rota rod test

Gosavi¹,

Kamdi²,

Kalambe³

et al. 2020

IJPP

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Background: The central nervous system (CNS) depressant effect is caused by the numerous sedative and hypnotic drugs that are currently used. Though in the late seventies some work was done on Withania coagulans, it is the vulnerable species not widely scattered. Therefore, it was worthy to investigate the CNS depressant activities of alcoholic extract of Withania coagulans fruits in Swiss albino mice by using rota rod test. Aim: To study the motor coordination activity of alcoholic extract of Withania coagulans fruits in Swiss albino mice by Rota Rod Test. Materials and Methods: Motor coordination was measured by using the Rota Rod Test. The CNS depressant drugs reduce the endurance time of mice on the rotating rod as they weaken the motor coordination so that mice fall early on the revolving rod. This endurance time is tested statistically among the control, standard and the test drugs. Statistical Analysis: One way ANOVA was used for the statistical analysis. Results: The endurance time in Swiss albino mice was statistically highly significantly (p-value < 0.001) associated with the alcoholic extract of Withania coagulans fruits on rota rod test. Conclusion:The alcoholic extract of Withania coagulans fruits established the CNS depressant activity in Swiss albino mice by rota rod test.

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“…9 Thereafter this plant was not much explored for the CNS DOI: http://dx.doi.org /10.18203/2319-2003.ijbcp20203617 activity, though lot of work was done on diabetes and other diseases. [10][11][12][13] Therefore, it was thought worthwhile to investigate neuropsychopharmacology parameter like anti-anxiety activity of Withania coagulans fruits aqueous extract using elevated plus maze (EPM). Mice have an aversion for high and open space and prefer enclosed space, thus spend more time in the enclosed arm.…”

Section: Introductionmentioning

confidence: 99%

The antianxiety activity of aqueous extract of Withania coagulans fruits in swiss albino mice by elevated plus maze

Gosavi

Kamdi²,

Kalambe³

et al. 2020

Int J Basic Clin Pharmacol

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Background: Several anti-anxiety drugs used today have the numerous side effects. The Withania coagulans a rare species, is not studied much for its actions on anxiety except in late seventies where it was explored for neuropsychopharmacology. Thus, it was thought commendable to explore the anti-anxiety activities of aqueous extract of Withania coagulans fruits in swiss albino mice using elevated plus maze test.Methods: Elevated plus maze (EPM) test was used for evaluating the anti-anxiety action of Withania coagulans fruits’ aqueous extract. If the extract had anti-anxiety action, then it was expected that the time spent in the open space would increase and that in the closed space would decrease. This increase in time in the open space, if found statistically significant, was considered for antianxiety action.Results: There was statistically highly significant (p value <0.001) association observed between the aqueous extract of Withania coagulans fruits with antianxiety action in the swiss albino mice by elevated plus maze test.Conclusions: The aqueous extract of Withania coagulans fruits did prove the antianxiety effect in the swiss albino mice by elevated plus maze test.

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In Silico and In Vitro Screening of Ethanolic Extract of Fruits of Withania Coagulans against Diabetes

Cited by 15 publications

References 0 publications

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The motor coordination activity of alcoholic extract of Withania coagulans fruits in Swiss albino mice by rota rod test

The antianxiety activity of aqueous extract of Withania coagulans fruits in swiss albino mice by elevated plus maze

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