<i>In vitro</i>activity of clarithromycin in combination with other antimicrobial agents against biofilm-forming<i>Pseudomonas aeruginosa</i>strains

Kádár, Béla; Szász, M.; Kristóf, Katalin; Pesti, N.; Krizsán, Gergely; Szentandrássy, Julianna; Rókusz, László; Nagy, K.; Szabó, Dóra

doi:10.1556/amicr.57.2010.3.8

Cited by 13 publications

(5 citation statements)

References 21 publications

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“…These results revealed a significant correlation between biofilm formation and MDR isolates [ 63 ]. It should be noted that, in contrast to these findings, some studies from different parts of the world indicated a lower prevalence of biofilm formation, and there was no correlation between antibiotic resistance and biofilm-producing [ 64 , 65 ]. This issue is possibly linked to other resistance mechanisms (efflux pumps, purines, chromosomal mutation, and plasmid acquisition) involved in antibacterial resistance [ 66 ].…”

Section: Discussionmentioning

confidence: 86%

Phenotype and genetic determination of resistance to common disinfectants among biofilm-producing and non-producing Pseudomonas aeruginosa strains from clinical specimens in Iran

et al. 2022

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Background Pseudomonas aeruginosa is a common pathogen in Hospitalized patients, and its various resistance mechanisms contribute to patient morbidity and mortality. The main aims of the present study were to assess the susceptibility of biofilm-producing and non-producing P. aeruginosa isolates to the five commonly used Hospital disinfectants, to evaluate the synergistic effect of selected disinfectants and Ethylene-diamine-tetra acetic acid (EDTA), and the effect of exposure to sub-inhibitory concentrations of Sodium hypochlorite on antimicrobial susceptibility test. Results The results showed that sodium hypochlorite 5% and Ethanol 70% were the most and least effective disinfectants against P. aeruginosa, respectively. The addition of EDTA significantly increased the effectiveness of the selected disinfectants. The changes in the antibiotic-resistance profiles after exposure to sub-inhibitory concentrations of disinfectants were observed for different classes of antibiotics (Carbapenems, Aminoglycosides, Cephalosporins, Fluoroquinolones). As well as near the all isolates harbored efflux pump genes and 117 (97.5%) of isolates produced biofilm. Conclusion In the current study, the mixture of disinfectant and EDTA were the most suitable selection to disinfect Hospital surfaces and instruments. Also, it was clear that exposure to sub-inhibitory concentrations of Sodium hypochlorite results in resistance to some antibiotics in P. aeruginosa species. Strong and intermediate biofilm formers belonged to MDR/XDR strains. Future studies should include more complex microbial communities residing in the Hospitals, and more disinfectants use in Hospitals.

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Section: Discussionmentioning

confidence: 86%

Phenotype and genetic determination of resistance to common disinfectants among biofilm-producing and non-producing Pseudomonas aeruginosa strains from clinical specimens in Iran

et al. 2022

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“…in 2010, 23.3% of sixty clinical samples of P. aeruginosa were positive for biofilm formation. [ 30 ] In another study performed by Ghadaksaz et al . in Iran, the frequency of biofilm formation was 50.1% among 104 clinical isolates of P. aeruginosa .…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Biofilm Formation and Frequency of Multidrug-resistant and Extended Drug-resistant Strain in Pseudomonas aeruginosa Isolated from Burn Patients in Isfahan

et al. 2018

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Background:Pseudomonas aeruginosa is a biofilm-forming bacterium which can result in serious health problems, particularly in burn patients. Biofilm has been assumed to protect the bacteria from environmental fluctuations such as antimicrobial agent. Mucoid strains generate extensive levels of the alginate exopolysaccharide, which is an important factor of its biofilm.Materials and Methods:Totally, 100 isolates of P. aeruginosa has been gathered from wound infections of burn patients. Polymerase chain reaction of exoA gene has been carried out to confirm the bacteriologic identification of isolates. The biofilm-forming capacity has been specified by capsule staining and microtiter plate test as qualitative and quantitative determination, respectively. Antimicrobial susceptibility of the isolates has been specified by disk diffusion method.Results:All the isolates carried the exoA gene. The antibiotic resistance was imipenem (90%); levofloxacin (93%); aztreonam (87%); piperacillin-tazobactam (85%); tobramycin (92%); polymyxin b (PB) (2%); and ceftazidime (CAZ) (32%). Totally, multidrug-resistant (MDR) and extended drug-resistant (XDR) isolates were 19% and 75%, respectively. Fortunately, pan drug-resistant (PDR) strain has not been observed. The assessment of biofilm formation has shown that 7% of the isolates were nonbiofilm (N), weak (W) 67%, moderate (M) 22%, and strong (S) 4%.Conclusions:As a result, the findings of this survey indicated that PB and CAZ were the most effective antibiotics against P. aeruginosa, which of course indicate a serious problem about the emergence of the PDR strains. There was no relationship between the patterns of biofilm production and antibiotic susceptibility, but high frequency of MDR/XDR and biofilm producer strains has been detected.

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“…Oncel et al 38 found that 60% (6/10) of P. aeruginosa isolates from chronic rhinosinusitis produced bacterial biofilms. Kádár et al 39 investigated the biofilm production of 60 P. aeruginosa strains isolated from clinical samples, and found that 23.3% of P. aeruginosa strains were biofilm positive. To our knowledge, there are no ophthalmic reports demonstrating the biofilm-forming capacity of P. aeruginosa clinical isolates from large sample size.…”

Section: Discussionmentioning

confidence: 99%

Biofilm-Forming Capacity ofStaphylococcus epidermidis,Staphylococcus aureus, andPseudomonas aeruginosafrom Ocular Infections

Hou¹,

Sun²,

Wang³

et al. 2012

Invest. Ophthalmol. Vis. Sci.

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Ophthalmic isolates of S. epidermidis and S. aureus could produce biofilms in vitro, whereas clinical strains of P. aeruginosa could not. The bacterial strains possess the genetic ability to produce biofilms, but that does not necessarily mean that biofilms will be produced. The knowledge of the process of bacterial adhesion suggests that a timely and appropriate intervention strategy be implemented in the early stages of biofilm-mediated infections.

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In vitroactivity of clarithromycin in combination with other antimicrobial agents against biofilm-formingPseudomonas aeruginosastrains

Cited by 13 publications

References 21 publications

Phenotype and genetic determination of resistance to common disinfectants among biofilm-producing and non-producing Pseudomonas aeruginosa strains from clinical specimens in Iran

Phenotype and genetic determination of resistance to common disinfectants among biofilm-producing and non-producing Pseudomonas aeruginosa strains from clinical specimens in Iran

Evaluation of Biofilm Formation and Frequency of Multidrug-resistant and Extended Drug-resistant Strain in Pseudomonas aeruginosa Isolated from Burn Patients in Isfahan

Biofilm-Forming Capacity ofStaphylococcus epidermidis,Staphylococcus aureus, andPseudomonas aeruginosafrom Ocular Infections

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