In vitro and in vivo studies on bacteria and encrustation resistance of heparin/poly-L-lysine-Cu nanoparticles coating mediated by PDA for ureteral stent application

Abstract

Ureteral stents are commonly utilized as a medical device to aid the flow of urine. However, biofilm formation and encrustation complications have been clinical problems. To overcome this challenge, heparin/poly-L-lysine-copper (Hep/PLL-Cu) nanoparticle was immobilized on a dopamine-coated polyurethane surface (PU/NPs). The stability and structural properties of the nanoparticles were characterized by Zeta potential, poly dispersion index, transmission electron microscopy, atom force microscopy, and contact angle. The surface composition, antibacterial potency, encrustation resistance rate, and biocompatibility of PU/NPs were investigated by scanning electron microscope, XPS, antibacterial assay, and MTS assay, respectively. In addition, the anti-encrustation property was studied by implanting coated NPs stents in the rat bladder for 7 days. It was shown that the size and distribution of Hep/PLL-Cu nanoparticles were uniform. PU/NPs could inhibit Proteus mirabilis proliferation and biofilm formation, and exhibit no cytotoxicity. Less encrustation (Ca and Mg salt) was deposited both in vitro and in vivo on samples, demonstrating that the NPs coating could be a potential surface modification method of ureteral material for clinical use.

Surface Modification of Zn–Cu Alloy with Heparin Nanoparticles for Urinary Implant Applications

“…Hence, the objective of this paper was to investigate the issue of repeated surgery and the corresponding complications associated with ureteral stents. It has been studied by our previous research that the Hep/PLL-Cu nanoparticles inhibited microbe proliferation and crystallization formation. , In the present study, Zn–Cu alloy and its surface modification by Hep/PLL nanoparticles (NPs) were prepared and immobilized, which were further investigated for biodegradability and biofunction in the urinary system in vitro and in vivo, respectively.…”

“…It has been studied by our previous research that the Hep/PLL-Cu nanoparticles inhibited microbe proliferation and crystallization formation. 27,33 In the present study, Zn−Cu alloy and its surface modification by Hep/PLL nanoparticles (NPs) were prepared and immobilized, which were further investigated for biodegradability and biofunction in the urinary system in vitro and in vivo, respectively. Zn−Cu alloy has been reported to exhibit excellent strength, ductility, moderate degradation, and acceptable biocompatibility.…”

“…It has been studied in our previous research that the anionic charge originating from the sulfate group of the heparin molecule interacted with Ca 2+ , exerting an inhibitory effect on the processes of nucleation, growth, and aggregation of crystals. 27 Thus, this present study aimed at investigating the property of a Zn−Cu alloy coated with heparin as a potential ureteral stent material against infection and encrustation in both in vitro and animal experiments. To that end, in order to determine its suitability for urological applications, the microstructure and in vitro corrosion behavior of the alloy were investigated.…”

Influence of a Composite Polylysine-Polydopamine-Quaternary Ammonium Salt Coating on Titanium on Its Ostogenic and Antibacterial Performance

“…When θ is greater than 90°, the surface of the material cannot be wetted by liquid ( Figure 14 b), which is hydrophobic. The larger the θ angle, the higher the hydrophobicity of the surface [ 41 , 42 , 43 , 44 , 45 ].…”

In vitro and in vivo study of antibacterial and anti‐encrustation coating on ureteric stents

“…Normally, bacteria in the urethra and urine are flushed away during urination; however, scaffold implantation can provide an abiotic surface for these bacteria, thus increasing their ability to adhere, colonise and form a biofilm layer [5]. Bacterial colonisation rate on scaffolds can reach 65% and 80% after implantation for 2-3 and 3-4 months, respectively [6,7]. Bacteria can ascend along a ureteric stent and enter the kidneys from the bladder, resulting in high rates of contamination and bacteriuria [8].…”