In Vitro Anthelmintic Activity of Crude Extracts of Aerial Parts of Cissus quadrangularis L. and Leaves of Schinus molle L. against Haemonchus contortus

Zenebe, Selamawit; Feyera, Teka; Assefa, Solomon

doi:10.1155/2017/1905987

Cited by 38 publications

(21 citation statements)

References 18 publications

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“…Moreover, for in vitro studies, H. contortus is proved to be a good test worm because of its longer survival in PBS. This abomasal helminth has recently been used for in vitro studies by other workers [23,24].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Anthelmintic Activity of Crude Extracts of Artemisia herba-alba and Punica granatum against Haemonchus contortus

Ahmed

Ejo

Feyera

et al. 2020

Journal of Parasitology Research

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Gastrointestinal nematodes (GINs) are the major limiting factor for the successfulness of livestock production throughout the world. Emergence of resistance strains as well as scarcity and high cost of the currently available drugs has led to the evaluation of other alternative helminth control options, mainly from plants. The current study is aimed at investigating the in vitro anthelmintic efficacy of crude methanolic extracts of two traditionally important medicinal plants, Artemisia herba-alba and Punica granatum, against Haemonchus contortus using adult motility assay (AMA) and egg hatch inhibition assay (EHIA). Four graded concentrations of the extracts were tested for both the AMA (10, 5, 2.5, and 1.25 mg/mg) and EHIA (0.1, 0.25, 0.5, and 1 mg/mL) in replicates. Albendazole and phosphate-buffered saline (AMA) or distilled water (EHIA) were used as the positive and negative controls, respectively. The crude extracts of A. herba-alba and P. granatum exhibited a potential anthelmintic activity at all dose levels in a concentration- and time-dependent fashion. The highest concentration (10 mg/mL) of all the extracts caused a significantly (p<0.05) superior nematocidal activity compared to the negative control. Moreover, significant and concentration-dependent egg hatching inhibition effect was observed from both plant extracts. Maximal (98.67%) egg hatching inhibition effect was exhibited by the flower extract of A. herba-alba at 1 mg/mL concentration. The relative egg hatch inhibition efficacy indicated that both plants caused a significantly (p<0.05) greater egg hatch inhibition within 48 hr of exposure. The current study validated the traditional use of both plants as a natural anthelmintic against H. contortus justifying a need to undertake detail pharmacological and toxicological investigation on both plants.

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Section: Discussionmentioning

confidence: 99%

In Vitro Anthelmintic Activity of Crude Extracts of Artemisia herba-alba and Punica granatum against Haemonchus contortus

Ahmed

Ejo

Feyera

et al. 2020

Journal of Parasitology Research

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“…The ultra-high-resolution mass spectrometry (UHRMS) analysis of the bioactive compounds in Mix1 and Mix2 also identified protoberberine-type alkaloids. Alkaloids in herbal species are commonly poisonous [37], but protoberberine alkaloids have a large variety of biological and pharmacological [38] and anthelmintic [39, 40] activities. The anthelmintic activity of herbs has sometimes been ascribed to alkaloids, which can interfere with DNA synthesis in parasites [41].…”

Section: Discussionmentioning

confidence: 99%

Natural chemotherapeutic alternatives for controlling of haemonchosis in sheep

et al. 2019

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Background Parallel in vitro and in vivo experiments were designed to evaluate promising chemotherapeutic alternatives for controlling haemonchosis in ruminants. In vitro anthelmintic activities (egg hatch test – EHT; larval development test – LDT) of aqueous and methanolic herbal extracts Mix1 and Mix2 were investigated. The in vivo effects of dietary supplementation with Mix1 and Mix2 on the parasitological status, inflammatory response, antioxidant parameters and microbial community of the lambs infected experimentally with Haemonchus contortus were investigated . Lambs were divided into four groups for the in vivo study: uninfected control lambs (C), infected lambs (I), infected lambs supplemented with Mix1 (I + Mix1) and infected lambs supplemented with Mix2 (I + Mix2). The experimental period was 70 days. Results The number of eggs per gram (EPG) of feces was quantified 22, 30, 37, 44, 51, 58, 65 and 70 days post-infection, and mean abomasal worm counts were assessed 70 days post-infection. Quantitative analyses identified 57.3 and 22.2 mg/g phenolic acids, 41.5 and 29.5 mg/g flavonoids and 1.4 and 1.33 mg/g protoberberine-type alkaloids in Mix1 and Mix2, respectively. The methanolic extracts of the herbal mixtures in both in vitro tests had higher anthelmintic effects ( P < 0.01) than the aqueous extracts, but the effects did not differ significantly between Mix1 and Mix2 ( P > 0.05). I + Mix1 and I + Mix2 lowered mean EPGs between 44 and 70 d by 58.1 and 51.6%, respectively. The level of IgG antibodies against H. contortus increased significantly after infection in each infected group. Conclusion These results represent the first monitoring of the in vitro anthelmintic effects of herbal mixtures on H. contortus . The in vivo experiment indicated that the anthelmintic effect was not sufficient for the elimination of parasites, but this herbal treatment may affect the host over a longer term, reducing the parasitic infection in the host.

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“…Methanolic extracts of aerial parts of C. quadrangularis were tested for in vitro anthelmintic activity against Haemonchus contortus, the causative agent of Haemonchosis, is a nematode parasite that feeds on blood of small ruminant animals and causes anaemia, anorexia, reduced growth, and eventual death of host animals [14]. The extract produced a dose dependent anthelmintic activity in both adult motility assay and egg hatch inhibition assay.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

A review on efficacy of Cissus quadrangularis in pharmacological mechanisms

Zaki¹,

Malathi²,

Latha³

et al. 2020

Int J Clin Microbiol Biochem Technol

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Cissus quadrangularis a succulent vine belongs to Vitaceae family is widely distributed throughout tropical and subtropical regions of the world and used frequently to various disorders. The plant has been reported to contain flavonoids, triterpenoids, phytosterols, glycosides and rich source of calcium. This study aims to bring a systematic review of C. quadrangularis in various pharmacological mechanisms. Evidence from the previous studies suggested the efficacy of C. quadrangularis with antimicrobial, anti-diabetic, anti-inflammatory, anti-obesity, anti-oxidant, bone turnover, cardiovascular and hepatoprotective activities. In conclusion, Cissus quadrangularis appears worthy of pharmacological investigations for new drug formulations.

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In Vitro Anthelmintic Activity of Crude Extracts of Aerial Parts of Cissus quadrangularis L. and Leaves of Schinus molle L. against Haemonchus contortus

Cited by 38 publications

References 18 publications

In Vitro Anthelmintic Activity of Crude Extracts of Artemisia herba-alba and Punica granatum against Haemonchus contortus

In Vitro Anthelmintic Activity of Crude Extracts of Artemisia herba-alba and Punica granatum against Haemonchus contortus

Natural chemotherapeutic alternatives for controlling of haemonchosis in sheep

A review on efficacy of Cissus quadrangularis in pharmacological mechanisms

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