In Vitro Anthelmintic Activity of Methanolic Extract from Caesalpinia coriaria J. Willd Fruits against Haemonchus contortus Eggs and Infective Larvae

Abstract

The aim of this study was to evaluate the in vitro lethal effect of a methanolic extract (ME) from Caesalpinia coriaria fruits against Haemonchus contortus eggs and infective larvae. The anthelmintic activity was assessed using the egg hatching inhibition assay (EHI) and the mortality test. The ME was assessed using five concentrations as follows: 6.15, 3.12, 1.56, and 0.78 mg/mL to eggs and 150, 100, 75, and 50 mg/mL to larvae, respectively. Ivermectin (5 mg/mL) was used as positive control and 4% methanol and distilled water were used as negative controls. The data of ovicidal and larvicidal effect were analyzed with a completely randomized design through ANOVA analysis using the general linear model (GLM) and lethal concentrations (LC50 and LC90) were estimated through a Probit analysis using the SAS program. A clear ME increased concentration dependence effect was observed in the EHI and mortality tests. The highest activity of the methanolic extract was observed at the highest concentration (P < 0.05) to obtain a similar effect to the positive control (ivermectin), with LC50 = 78.38 and 0.00064 mg/mL and LC90 =235.63 and 0.024 mg/mL, respectively, for larvae and eggs. The results indicate that the C. coriaria fruit ME possesses in vitro ovicidal and larvicidal properties (gallotannins: methyl gallate) against H. contortus that needs to be investigated more in vivo for the control of gastroenteric nematodes in ruminants.

In Vitro Anthelmintic Activity of Crude Extracts of Artemisia herba-alba and Punica granatum against Haemonchus contortus

“…Plant materials tested for their in vitro anthelmintic activity in the present study have been identified by local livestock raisers. In vitro techniques such as the AMA and EHIA are preferred to in vivo methods due to their low cost, simplicity, and rapid turnover [23]. Moreover, for in vitro studies, H. contortus is proved to be a good test worm because of its longer survival in PBS.…”

“…Moreover, for in vitro studies, H. contortus is proved to be a good test worm because of its longer survival in PBS. This abomasal helminth has recently been used for in vitro studies by other workers [23,24].…”

In vitro anthelmintic activity of aqueous and ethanol extracts of Paraserianthes falcataria bark waste against Haemonchus contortus obtained from a local slaughterhouse in Indonesia

“…The studies on flavonoid derivatives proved that flavonoids have antibacterial, anthelmintic, antiviral, anti-inflammatory, anti-allergic, and anticancer activities. Plant active compounds were proved to suppress H. contortus [28][29][30][31]. Badar et al [32] stated that the variation in anthelmintic sericea leaf extract and agrimol G component [37].…”

In vitro antimicrobial activity of Caesalpinia coriaria (Jacq.) Willd extracts on Streptococcus pyogenes and Candida albicans

“…The C. coriaria material was collected in the Toroqui community (which belongs to Wayuu community) in the rural area of the municipality of Riohacha, La Guajira (11˚29ʼ51.7 "N & 072˚50ʼ03.0" W) approximately 142.91 m 2 , the area is classified as tropical dry forest (16). The plant sample consisted of 500 g of ripe fruits of C. coriaria, applying a standard technique (17). First, the fruits were dried in a shadow and crushed to pulverize.…”