<i>In vitro</i> anti-inflammatory activity of the <i>Artemisia montana</i> leaf ethanol extract in macrophage RAW 264.7 cells

Jeong, Seong Hun; Kim, Jisu; Min, Hye‐Young

doi:10.1080/09540105.2018.1439454

Cited by 19 publications

(13 citation statements)

References 34 publications

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“… 32 Previous studies on the anti-inflammatory of Artemisia species were stated. Testing extract of A. annua , 33 A. fukudo , 34 and A. montana 35 on murine RAW 264.7 macrophage cells showed anti-inflammatory activity by inhibiting LPS-induced nitric oxide and prostaglandin E 2 productions. Our A. lactiflora extract might also have those similar anti-inflammatory activities.…”

Section: Discussionmentioning

confidence: 92%

Artemisia lactiflora Extracts Prevent Inflammatory Responses of Human Macrophages Stimulated with Charcoal Pyrolysis Smoke

Kooltheat

Chujit

Nuangnong

et al. 2021

J Evid Based Complementary Altern Med

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Artemisia lactiflora, a Chinese-origin plant, has been reported to have unique phytochemicals responsible for its medicinal properties. The growth of the agricultural industry emits air pollution, which has adverse effects on health. There are limited scientific reports on the biological activities of A. lactiflora. Studies on its activities and mechanisms may provide insight into its use in medicinal purposes to treat those health problems and conditions. In this study, leaves of A. lactiflora were extracted and fractioned with solvents of different polarities. Total phenolics, total flavonoids DPPH• scavenging, ABTS•+ scavenging, and cytotoxicity of A. lactiflora were assessed. Anti-inflammatory activities were evaluated by pre-treating macrophages with extract or fractions then induced inflammatory response by coconut shell pyrolysis smoke. Inflammatory responses were assessed by measuring pro-inflammatory genes expression and pro-inflammatory cytokines secretion. Among all extract and fractions of A. lactiflora, butanol fraction has the highest phenolic, flavonoid, and DPPH• scavenging activity. All extract and fractions significantly down-regulated pro-inflammatory genes expression ( RelA, TNF, IL6) and decreased pro-inflammatory cytokines secretion (TNF-α, IL-6), p < 0.0001, compared with pyrolysis smoke-induced macrophages. The ethyl acetate fraction showed the highest anti-inflammatory activity in decreasing pro-inflammatory cytokines secretion. These results may prove the anti-inflammatory activities of A. lactiflora through the inhibition of the NF-κB-dependent pathway. Taken together, this study first reported the anti-inflammatory activities of A. lactiflora. Thus, the plant can be used to prevent and treat inflammatory responses caused by highly oxidative pyrolysis smoke released from the re-utilization of agro-industrial leftovers.

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Section: Discussionmentioning

confidence: 92%

Artemisia lactiflora Extracts Prevent Inflammatory Responses of Human Macrophages Stimulated with Charcoal Pyrolysis Smoke

Kooltheat

Chujit

Nuangnong

et al. 2021

J Evid Based Complementary Altern Med

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“…Macrophages are known to play a critical role in innate immunity and attack, destroy and ingest foreign substances by producing cytokines and NO (Jeong, Kim, & Min, 2018). In this study, we investigated the immuno-stimulatory effects of L-EPS on RAW264.7 cells by examining the phagocytic activity and the production of NO and cytokines.…”

Section: Discussionmentioning

confidence: 99%

Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo

Xiu

Zhang

et al. 2018

Food and Agricultural Immunology

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Exopolysaccharides act as mediators of cross-talk between probiotics and the host. Here, we found that EPS derived from probiotic Lactobacillus casei WXD030 strain (L-EPS) could modulate immune responses in vitro and in vivo. L-EPS could significantly enhance the proliferation and phagocytic activity as well as induce the production of NO, TNF-α, IL-1β and IL-6 in RAW264.7 cells. Furthermore, L-EPS could induce the maturation of BMDCs. In addition, L-EPS could largely increase the titres of OVA-specific antibodies and markedly enhanced T cell proliferation. Notably, L-EPS also increased expression of IL-4 and INF-γ expression in CD4 + T cells. Consistently, when used as an adjuvant in vivo with the foot-and-mouth disease vaccine, L-EPS largely enhanced the FMDVspecific antibody production. Collectively, these results suggested that L-EPS derived from probiotic L. casei strain had adjuvant activity, which may be a safe and efficacious adjuvant candidate suitable for a wide spectrum of prophylactic and therapeutic vaccines.

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“…The present research showed that the extract of Artemisia fruit contains flavonoids, which capture free radicals (Andriantsitohaina, 1999, Baumann et al, 1980 and many other active compounds which activate the enzymes and transporter proteins in the membrane of the cell (Kotani et al, 2000). Since Artemisia has better phytochemical compounds (Kooti et al) which may have role in more activities (Jeong et al, 2018, Vijeesh et al, 2017. Moreover, this study showed the difference between hot and cold extract of Artemisia fruit.…”

Section: Resultsmentioning

confidence: 71%

Artemisia L. Fruit Extract Normalized Diabetic and Liver Function Parameters in Streptozotocin-Induced Diabetic Mice

Eltayef¹,

Jouda²,

Saeed³

et al. 2020

ATMPH

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Many other researchers studied the effect of many part from Artemisia but not fruit on the diabetes. Artemisia fruits were used in this study, they had been dried and grinded then some chemical analyses were done to determine the active compounds of the fruits in both hot and cold extract. Since our results shows that the hot extract has more active compounds from the cold, the hot extract of Artemisia L. fruit were used in this study to determine the efficacy of it as diabetic treatment in streptozotocin-induced diabetic mice. Streptozocin (STZ) was used to induce diabetes in 35 mice which divided to 5 groups. One group consumed normal saline while the other four consumed Artemisia with four different concentrations. Another 10 mice weren't injected with STZ which were they had been divided to two groups; one consumed Artemisia and the other consumed normal saline. The fasting blood sugar (FBS) was determined on different period; zero time, 7 and 14 days after treatment. The mice were scarified and blood was collected. Insulin, GOT and GPT levels were determine in serum. FBS was higher in all STZ-induced diabetic mice dropped to normal after 14 days. The FBS of mice, treated with 500 Artemisia/ kg mice, was closer to control. Insulin level was lower in STZ-induce diabetic mice without treatment raised to normal after treatment with 500 and 1000 mg Artemisia/ kg mice. Same results were found for GOT and GPT levels. As conclusion, Artemisia appears to be a good modulator for diabetes especially at 500 and 1000 mg Artemisia/ kg body weight.

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In vitro anti-inflammatory activity of the Artemisia montana leaf ethanol extract in macrophage RAW 264.7 cells

Cited by 19 publications

References 34 publications

Artemisia lactiflora Extracts Prevent Inflammatory Responses of Human Macrophages Stimulated with Charcoal Pyrolysis Smoke

Artemisia lactiflora Extracts Prevent Inflammatory Responses of Human Macrophages Stimulated with Charcoal Pyrolysis Smoke

Immunostimulatory activity of exopolysaccharides from probiotic Lactobacillus casei WXD030 strain as a novel adjuvant in vitro and in vivo

Artemisia L. Fruit Extract Normalized Diabetic and Liver Function Parameters in Streptozotocin-Induced Diabetic Mice

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