<i>In Vitro</i>Antiplasmodial Activity of Callus Culture Extracts from Fresh Apical Stems of<i>Phyllanthus niruri</i>: Part 1

Luyindula, N.; Tona, L.; Lunkebila, S.; Tsakala, M.; Mesia, K.; Musuamba, C.T.; Cimanga, R.K.; Apers, Sandra; Bruyne, T. De; Pieters, Luc; Vlietinck, A. J.

doi:10.3109/13880200490893177

Cited by 13 publications

(12 citation statements)

References 28 publications

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“…The plant showed antispasmodic, laxative, diuretic, carminative, antiviral and hypoglycemic properties (Nadkarni, 1993); its antiviral activity also extends to human immuno-deficiency virus-1 reverse transcriptase inhibition (Notka, Meier & Wagner, 2004). Crude plant as well as callus culture extracts of Phyllanthus exhibited in vitro antiplasmodial activity (Luyindula et al, 2004). Also the plant extract caused inhibition of calcium oxalate internalization in urolithiasis (Campos & Schor, 1999).…”

Section: Introductionmentioning

confidence: 99%

Hairy root extract of Phyllanthus amarus induces apoptotic cell death in human breast cancer cells

Abhyankar

Suprasanna

Pandey

et al. 2010

Innovative Food Science & Emerging Technologies

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Section: Introductionmentioning

confidence: 99%

Hairy root extract of Phyllanthus amarus induces apoptotic cell death in human breast cancer cells

Abhyankar

Suprasanna

Pandey

et al. 2010

Innovative Food Science & Emerging Technologies

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“…The geographic situations for those localities were illustrated in Figure 1 and Table 1. Callus was obtained by in vitro cell culture (Luyindula et al, 2004). The botanical identification was assured by M. Nlandu of the INERA (Institut National pour l'Etude et la Recherche Agronomiques), Herbarium -University of Kinshasa.…”

Section: Plant Materialsmentioning

confidence: 99%

“…Considering the profile of the peaks of callus which are almost similar to those of roots, we can suggest that callus would present the same in vitro antiplasmodial activity than roots. However, Cimanga et al (2004) and Luyindula et al (2004) have shown that in vitro antiplasmodial activity of callus varied with the number of months or plant parts used in vitro cell culture.…”

Section: High Performance Liquid Chromatographymentioning

confidence: 99%

Phytochemical analysis of Phyllanthus niruri L. (Phyllanthaceae) extracts collected in four geographical areas in the Democratic Republic of the Congo

Nakweti¹,

Ndiku²,

Doumas

et al. 2013

Afr. J. Plant Sci.

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This study aims at investigating the phytochemical analysis or to analyze the secondary metaboliotes of Phyllanthus niruri L. plants from four collection sites which University of Kinshasa (Unikin), National Pedagogic University of Kinshasa (UPN), Kimwenza (Kim) and Kisantu (Kis) in the Democratic Republic of the Congo (DRC). This study should give an explanation about the change of antiplasmodial activity of the same plant depending on the location of harvest. The samples of P. niruri were analyzed by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) for secondary metabolites characterizations such as of flavonoids, saponins and steroidal sapogenins and others phenolic compounds. The results revealed that UPN location presented more peaks (22) than Unikin (20), Kimwenza (15) and Kisantu (12). But Unikin location revealed more peaks (7) corresponding to major compounds than samples from others locations (UPN: 5; Kimwenza: 2 and Kisantu: 4). The peak 1 of UPN is higher (13.73) comparing to all peaks samples. The yellow-colored spots were present at all samples but those of UPN were more accentuated than all. In Kimwenza samples, two others colored spots (violet and blue) were presented. It suggested that in vitro antiplasmodial activity would be based on compounds eluted probably at the retention time around 22 min. Sometime the compounds eluted at 4.28 and 7.8 min contribute to in vitro antiplasmodial activity. The results revealed again the presence of the saponins or the steroidal sapogenins in P. niruri, made for the characterization by HPLC or by TLC probables flavonoids and presence of steroidal sapogenins.

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“…P. odontadenius is one of most important medicinal plants in the genus Phyllanthus (Phyllanthaceae) used in different regions in the world for the treatment of various diseases (Unander et al, 1991;Bajaj, 1999;Luyindula et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Hairy roots production in Phyllanthus odontadenius Mll. Arg. by seedlings transformed with Agrobacterium rhizogenes A4RS/pHKN29

Nakweti¹,

Vaissayre²,

Ndofunsu³

et al. 2015

Afr. J. Plant Sci.

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Phyllanthus odontadenius Müll. Arg is one of the most important medicinal plants in the genusPhyllanthus (Phyllanthaceae). It is used in different regions in the world for the treatment of various diseases for example malaria caused by Plasmodium falciparum causing millions deaths in tropical and subtropical regions. In this work, we experimented P. odontadenius seeds germination and seedlings infected by Agrobacterium rhizogenes A4RS/pHKN9. We obtained 36% of seeds that germinated for 84.6% of seedlings transformed which showed positive gfp activities. The number of hairy roots formed in infectious sites for seedlings infected with A. rhizogenes A4RS/pHKN29 is valued at 6.3±2.71 against 0 for control seedlings. Diagrams showed that mean length for the new-formed roots were 1.92±0.55 cm and 1.59±0.49 cm for hairy root.

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In VitroAntiplasmodial Activity of Callus Culture Extracts from Fresh Apical Stems ofPhyllanthus niruri: Part 1

Cited by 13 publications

References 28 publications

Hairy root extract of Phyllanthus amarus induces apoptotic cell death in human breast cancer cells

Hairy root extract of Phyllanthus amarus induces apoptotic cell death in human breast cancer cells

Phytochemical analysis of Phyllanthus niruri L. (Phyllanthaceae) extracts collected in four geographical areas in the Democratic Republic of the Congo

Hairy roots production in Phyllanthus odontadenius Mll. Arg. by seedlings transformed with Agrobacterium rhizogenes A4RS/pHKN29

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