In vitro cytotoxicity of maxillofacial silicone elastomers: Effect of accelerated aging

Abstract

The purpose of this in vitro study was to evaluate the cytotoxicity of three maxillofacial silicone elastomers at 24, 48, and 72 h on L-929 cells and to determine the effect of accelerated aging on the cytotoxicity of these silicone elastomers. Disc-shaped test samples of maxillofacial silicone elastomers (Cosmesil, Episil, Multisil) were fabricated according to manufacturers' instructions under aseptic conditions. Samples were then divided into three groups: (1) not aged; (2) aged for 150 h with an accelerated weathering tester; and (3) aged for 300 h. Then the samples were placed in Dulbecco's Modified Eagle Medium/Ham's F12 (DMEM/F12) for 24, 48, and 72 h. After the incubation periods, cytotoxicity of the extracts to cultured fibroblasts (L-929) was measured by MTT assay. The degree of cytotoxicity of each sample was determined according to the reference value represented by the cells with a control (culture without sample). Statistical significance was determined by repeated measurement ANOVA (p < 0.01) followed by Duncan's test (p < 0.05). All test materials in each group demonstrated high survival rates in MTT assay (Episil; 93.84%, Multisil; 88.30%, Cosmesil; 87.50%, respectively); however, in all groups, Episil material demonstrated significantly higher cell survival rate after each of the experimental incubation periods (p < 0.05). Accelerated aging for 150 and 300 h had no significant effect on the biocompatibility of maxillofacial silicone elastomers tested (p > 0.05).

Biomechanical properties of nano-TiO2 addition to a medical silicone elastomer: The effect of artificial ageing

“…Other researchers have studied the biocompatibility of room-temperature silicone elastomers using the MTT assay, 42,43 and concluded that cell survival in the presence of silicone elastomers was affected by incubation periods. In our current study, we found that cell survival increased proportionally with the length of the incubation period.…”

Do novel cement-type biomaterials reveal ion reactivity that affects cell viability in vitro?

“…The MTT test is considered to be a sensitive quantitative method of evaluating cell viability and proliferation in vitro [14,15]. Yellow tetrazolium salt (MTT) is reduced to purple formazan in the living cells or cells in early apoptosis cycle with active mitochondrial dehydrogenases [16,17].…”

In Vitro Cytotoxicity of Maxillofacial Silicone Elastomers: Effect of Nano‐particles

“…Since silicone elastomer was first successfully used as a prosthetic material in 1960, it has become the first choice maxillofacial prosthetic material for soft tissue repair . Silicone elastomers have been generally used to restore facial prostheses because of their chemical and mechanical properties, strength, ease of manipulation, pigmentability, and durability . With regard to ideal qualities (chemical, physical, and mechanical) of a prosthetic material, it should be compatible with human tissues and cause no irritation .…”

“…Silicone elastomers have been generally used to restore facial prostheses because of their chemical and mechanical properties, strength, ease of manipulation, pigmentability, and durability . With regard to ideal qualities (chemical, physical, and mechanical) of a prosthetic material, it should be compatible with human tissues and cause no irritation . The material must not be capable of initiating an inflammatory or foreign body reaction, and it should be noncarcinogenic .…”

“…With regard to ideal qualities (chemical, physical, and mechanical) of a prosthetic material, it should be compatible with human tissues and cause no irritation . The material must not be capable of initiating an inflammatory or foreign body reaction, and it should be noncarcinogenic . Although there have been improvements in the last few decades in facial prosthetic materials, silicone elastomers have certain deficiencies that reduce the clinical longevity of the prostheses.…”