In vitro function of platelet concentrates prepared after filtration of whole blood or buffy coat pools

Abstract

Our data indicates that the in vitro characteristics of PCs prepared using these methods is maintained over storage for 7 days in plasma and is not associated with significant deterioration of platelet function. ONE SENTENCE SUMMARY: In vitro function of platelet concentrates prepared by either filtration of whole blood, or pooled buffy coats.

Storage of platelet concentrates from pooled buffy coats made of fresh and overnight‐stored whole blood processed on the novel Atreus 2C+ system: in vitro study

“…In our study, the percentage of PLTs expressing CD42b were all higher (>91% on Day 7) than those associated with poor in vivo recovery in the mentioned study. We found no statistical difference in the percentage of PLTs expressing CD42b between the groups and our findings are in accordance with those of others who have shown that storage of PLTs reduces the expression of GP1bα on the surface of the PLTs 36‐38 . This reduction is assumed to decrease the adhesive capacity.…”

Measurement of phosphatidylserine exposure during storage of platelet concentrates using the novel probe lactadherin: a comparison study with annexin V

“…Similar to some previous studies on PCs stored up to 7 days, 4,31 we observed a decrease in GPIb expression without significant change in the percentage of PLTs expressing this glycoprotein. On the other hand, CD61 expression remained essentially unchanged, which is in line with many previous studies 4,32 …”

Development of a quality monitoring program for platelet components: a report of the first four years' experience at Canadian Blood Services

“…The timing of the QMP allowed an overview of the implementation of a new production method across the entire organization. A study conducted at a single site during the production method changeover between PRP PCs and the BC production method found that BC PCs have laboratory variables that suggest they are of higher quality than those produced by the PRP method, 7 a finding that agrees with other studies 21‐24 . Via QMP, it was noted that, in general, variances among in vitro measures were lower in BC PCs versus PRP PCs, indicating that the pooled BC method produces a more consistent product and that the new method had been successfully implemented across most sites.…”