<i>In vitro</i>
            germination and biochemical profiling of
            <i>citrus reticulata</i>
            in response to green synthesised zinc and copper nanoparticles

Hussain, Mubashir; Raja, Naveed Iqbal; Mashwani, Zia‐ur‐Rehman; Iqbal, Muhammad; Ejaz, Muhammad; Yasmeen, Farhat; Sohail, Mohammad

doi:10.1049/iet-nbt.2016.0256

Cited by 40 publications

(25 citation statements)

References 36 publications

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“…This extract was filtered and filtrate was stored at 4°C in refrigerator for further use. The protocol of Hussain et al [18] was considered for green synthesis of AgNPs. For this purpose, preparation of 1 mM solution of AgNO 3 was done by mixing 0.17 g AgNO 3 in 1 l distilled water.…”

Section: Methodsmentioning

confidence: 99%

Effect of silver nanoparticles and silver nitrate on growth of rice under biotic stress

Ejaz

Raja

Mashwani

et al. 2018

IET nanobiotechnol.

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This study was organised to check the effect of silver nanoparticles and silver nitrate on rice growth against biotic stress. Silver nanoparticles were synthesised by using plant extract as reducing agent, followed by characterisation through UV Vis spectroscopy, XRD, EDS and SEM. Aspergillus application significantly reduced rice plant fresh mass (0.9%), dry mass (0.21%), root length (2.3%), shoot length (5.2%) and root number (1%) in comparison to control. Similarly, leaf area, leaf fresh mass, dry mass and leaf number were also reduced by 23.1, 0.02, 0.11 and 0.9%, respectively. AgNPs and AgNO treatments increased the root length (16.2 & 12.8%), shoot length (21 & 20%), root number (8.1 & 6.8%), plant fresh weight (6.4 & 5%) and plant dry weight (4.6 & 3.5%) in 75mg/l treatment of AgNPs and AgNO respectively. Similarly, AgNPs and AgNO treatment (75 mg/l concentrations) reflected remarkable increase in leaf area (58.8 & 57.2 %), leaf number (4.3 & 3.7 %), leaf fresh weight (1.7 & 1.4 %) and leaf dry weight (0.9 & 0.8 %). Overall AgNPs showed more significant results as compared to AgNO. The quantity of aflatoxins ranged from 3.1 to 7.7 μg/kg against tolerable limit (4 µg/kg). Overall AgNPs and AgNO treatments showed significant results and it could be considered as a strategy for aflatoxin management in rice plants.

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Section: Methodsmentioning

confidence: 99%

Effect of silver nanoparticles and silver nitrate on growth of rice under biotic stress

Ejaz

Raja

Mashwani

et al. 2018

IET nanobiotechnol.

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“…The supernatant was discarded, and the lower pellet was again suspended in deionised water followed by centrifugation for 5 min. The process was repeated three times to remove impurities any present [18].…”

Section: Synthesis Of Znnpsmentioning

confidence: 99%

In vitro germination and biochemical profiling of Brassica napus in response to biosynthesised zinc nanoparticles

Sohail

Amara

Shad

et al. 2018

IET nanobiotechnol.

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With the progression of nanotechnology, the use of nanoparticles (NPs) in consumer products has increased dramatically and green synthesis is one of the cheapest and eco-friendly methods to obtain non-hazardous NPs. In the current research zinc (Zn) NPs synthesis was carried out by using the fresh and healthy leaves of Mentha arvensis L. followed by characterisation through ultraviolet (UV)-visible spectroscopy, X-ray diffraction (XRD) and scanning electron microscopy (SEM). UV-visible spectroscopy confirmed the green synthesis of ZnNPs, while XRD confirmed the size of NPs, which was 30-70 nm. SEM shows that the shape of ZnNPs was irregular. The effects of green synthesised NPs on two different varieties of Brassica napus were evaluated. Exposure to ZnNPs (5, 15, and 25 mg/l −1) caused a significant increase in root and shoot length of B. napus. The application of NPs significantly improved plant germination and triggered the production of secondary metabolite and antioxidant enzymes. ZnNPs showed a significant increase in chlorophyll, superoxide dismutase, total flavonoid content (TFC) and antioxidant enzymes while total phenolic content was decreased when TFC increased. Thus, it has been concluded from the current study that ZnNPs may possibly trigger the production of antioxidant enzymes and various biochemical compounds.

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“…Employment of ENPs in the tissue/cell culture industry of medicinal plants is limited despite a lot of progress having been made in the last decade in the agricultural nanotechnology sector. The available literature suggests that ENPs evoke abiotic stress in tissue/cell culture raised medicinal plants [43][44][45][46][47][48]. These studies illustrate the effects induced by ENPs on the growth and secondary metabolism of plants of medicinal significance.…”

Section: Discussionmentioning

confidence: 92%

Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)

et al. 2020

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Sustainable production of secondary metabolites in medicinal plants by artificial culturing on the industrial scale has gained worldwide importance. Engineered nanoparticles (ENPs) play a pivotal role in the elicitation of compounds of medicinal value. This investigation explores the influence of ZnO and CuO ENPs on in vitro roots formation, non-enzymatic antioxidant activities, and production of steviol glycosides (SGs) in regenerants of Candyleaf, Stevia rebaudiana. ENPs were applied in 0, 2, 20, 200, and 2000 mg/L of concentration in the MS medium containing plant shoots. The percentage of rooting induced was 91% and 94% by applying ZnO ENPs (2 mg/L) and CuO ENPs (20 mg/L), respectively. Moreover, at 2 mg/L of ZnO and 20 mg/L of CuO ENPs, the high performance liquid chromatography studies determined the significantly greatest content of SGs; rebaudioside A (4.42 and 4.44) and stevioside (1.28 and 1.96). Phytochemical studies including total flavonoid content, total phenolic content, and 2,2-diphenyl-1-picryl hydrazyl-free radical scavenging activity were calculated highest by the regenerants grown in 2 mg/L of ZnO and 20 mg/L of CuO ENPs dosage. Both ZnO and CuO ENPs at 200 mg/L and 2000 mg/L of concentration induced adverse effects on plant biomass, antioxidant activities, and SGs content up to 1.22 and 1.77 for rebaudioside A and 0.21 and 0.25 for stevioside. Hence, the biochemical and morphophysiological responses of Candyleaf were elicited as a defense against ZnO and CuO ENPs applied under threshold limit. This artificial biotechnological technique holds great promise for continued production of natural antioxidants on commercial scale and our study has further strengthened this impact.

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In vitro germination and biochemical profiling of citrus reticulata in response to green synthesised zinc and copper nanoparticles

Cited by 40 publications

References 36 publications

Effect of silver nanoparticles and silver nitrate on growth of rice under biotic stress

Effect of silver nanoparticles and silver nitrate on growth of rice under biotic stress

In vitro germination and biochemical profiling of Brassica napus in response to biosynthesised zinc nanoparticles

Engineered ZnO and CuO Nanoparticles Ameliorate Morphological and Biochemical Response in Tissue Culture Regenerants of Candyleaf (Stevia rebaudiana)

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