<i>In Vitro</i> Superfusion Method to Study Nerve‐Immune Cell Interactions in Human Synovial Membrane in Long‐standing Rheumatoid Arthritis or Osteoarthritis<sup>a</sup>

Miller, Luitpold E.; Wessinghage, D; Müller‐Ladner, Ulf; Schölmerich, Jürgen; Falk, Werner; Kerner, Thoralf; Straub, Rainer H.

doi:10.1111/j.1749-6632.1999.tb07649.x

Cited by 12 publications

(2 citation statements)

References 20 publications

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“…Therefore, the action of tramadol could be, at least in part, related to its opioid effect. Concerning possible other mechanisms, there is evidence that monoamines can play an important role as local factors of immunomodulation in inflammation [36]. It has been recently suggested that norepinephrine is able to inhibit secretion of proinflammatory mediators in synovial tissue of patients with OA [37].…”

Section: Discussionmentioning

confidence: 99%

Effects of tramadol on synovial fluid concentrations of substance P and interleukin-6 in patients with knee osteoarthritis: comparison with paracetamol

Bianchi

Broggini

Balzarini

et al. 2003

International Immunopharmacology

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Section: Discussionmentioning

confidence: 99%

Effects of tramadol on synovial fluid concentrations of substance P and interleukin-6 in patients with knee osteoarthritis: comparison with paracetamol

Bianchi

Broggini

Balzarini

et al. 2003

International Immunopharmacology

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“…Clinical observations demonstrate that, when RA patients, who almost always present symmetric upper limb involvement, develop hemiplegia, arthritis of the paralytic limb improves and it may even reach complete remission with resolution of bone erosions 11 . Sensory neurons release inflammatory peptides which could be involved in a pathway leading to symmetric arthritis and continuous pain stimulation 12 .…”

Section: Introductionmentioning

confidence: 99%

Neuroendocrine Modulation Induced by Selective Blockade of TNF‐α in Rheumatoid Arthritis

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Marinosci

Matteo

et al. 2006

Annals of the New York Academy of Sciences

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Tumor necrosis factor-alpha (TNFalpha) is a main actor in the pathogenesis of rheumatoid arthritis (RA), interacting with other molecules in complex mechanisms. The neuroendocrine system is known to be involved and Chromogranin A (CHGA) serum levels are elevated in patients with RA. We evaluated the effect of the selective blockade of TNF-alpha, induced by treatment with anti-TNF-alpha monoclonal antibodies (mAbs), on the serum levels of CHGA and on its correlation with TNF-alpha and TNF-alpha receptors (TNFRs) serum levels. Seven patients with RA have been treated with the anti-TNF-alpha mAb, infliximab. We measured the serum levels of TNF-alpha, its receptors (tumor necrosis factor receptor-I [TNFR-I] and tumor necrosis factor receptor-II [TNFR-II]), and CHGA before and during the treatment. We also measured, as a control, the serum levels of CHGA, TNF-alpha, and soluble TNFRs in 14 patients who were being treated with infliximab, adalimumab, or etanercept and in 20 matching negative controls. The serum levels of TNFR-I and TNFR-II, which are a sensitive marker for the TNF-alpha pathway, correlated with those of CHGA before treatment (Pearson's coefficient, respectively, 0.59 and 0.53). Treatment with anti-TNF-alpha mAb provided a significant clinical response in all patients and the correlation between CHGA and TNFR-I and TNFR-II was no more evident during treatment (respectively, -0.09 and -0.07). TNF-alpha blockade allows a clinical effect in patients with RA and modifies the correlation between CHGA and TNFRs, suggesting that TNF-alpha and CHGA reciprocally interfere in the pathogenesis of RA, through intermediate adaptors, whose identification warrants further studies.

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Increased prevalence of semaphorin 3C, a repellent of sympathetic nerve fibers, in the synovial tissue of patients with rheumatoid arthritis

Miller¹,

Weidler²,

Falk³

et al. 2004

Arthritis & Rheumatism

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ObjectiveThe presence of selective sympathetic nerve repellents, i.e., semaphorins, may be responsible for the observed reduction of sympathetic innervation in the synovial tissue of patients with rheumatoid arthritis (RA). This study was undertaken to investigate the presence of different semaphorins in synovial tissue of patients with RA, patients with osteoarthritis (OA), and control subjects without inflammation.MethodsIn situ hybridizations with digoxigenin‐labeled RNA probes directed against different semaphorins were performed. The presence of semaphorin 3C (S3C) in the synovial tissue of 10 RA, 10 OA, and 5 control subjects was investigated using a polyclonal antiserum directed against S3C.ResultsAll in situ hybridizations revealed the presence of S3C messenger RNA, but no other investigated semaphorin (i.e., against primary afferent sensory nerve fibers), in the synovial tissue of RA and OA patients. Immunohistologic double staining demonstrated that macrophages and fibroblasts were positive for S3C protein. Quantitative analysis of S3C protein staining showed an increased density of S3C‐positive cells in the synovial tissue of RA patients (mean ± SEM 339 ± 65 cells/mm2) in comparison with OA patients (168 ± 27/mm2; P = 0.031 versus RA) and controls (126 ± 26/mm2; P = 0.027 versus RA). Studies of the relationship between sympathetic nerve fiber density and S3C‐positive cell density in the tissue of all patients showed that RA patients generally had lower densities of sympathetic nerve fibers and higher densities of S3C‐positive cells than OA patients and control subjects.ConclusionThese findings suggest that S3C from macrophages and fibroblasts, which is selectively directed against sympathetic nerve fibers, could be one element responsible for reduced sympathetic innervation in RA tissue. The inability of sympathetic nerve fibers to reinnervate synovial tissue could contribute to the chronic nature of RA.

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In Vitro Superfusion Method to Study Nerve‐Immune Cell Interactions in Human Synovial Membrane in Long‐standing Rheumatoid Arthritis or Osteoarthritis^a

Cited by 12 publications

References 20 publications

Effects of tramadol on synovial fluid concentrations of substance P and interleukin-6 in patients with knee osteoarthritis: comparison with paracetamol

Effects of tramadol on synovial fluid concentrations of substance P and interleukin-6 in patients with knee osteoarthritis: comparison with paracetamol

Neuroendocrine Modulation Induced by Selective Blockade of TNF‐α in Rheumatoid Arthritis

Increased prevalence of semaphorin 3C, a repellent of sympathetic nerve fibers, in the synovial tissue of patients with rheumatoid arthritis

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In Vitro Superfusion Method to Study Nerve‐Immune Cell Interactions in Human Synovial Membrane in Long‐standing Rheumatoid Arthritis or Osteoarthritisa

Cited by 12 publications

References 20 publications

Effects of tramadol on synovial fluid concentrations of substance P and interleukin-6 in patients with knee osteoarthritis: comparison with paracetamol

Effects of tramadol on synovial fluid concentrations of substance P and interleukin-6 in patients with knee osteoarthritis: comparison with paracetamol

Neuroendocrine Modulation Induced by Selective Blockade of TNF‐α in Rheumatoid Arthritis

Increased prevalence of semaphorin 3C, a repellent of sympathetic nerve fibers, in the synovial tissue of patients with rheumatoid arthritis

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Product

Resources

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In Vitro Superfusion Method to Study Nerve‐Immune Cell Interactions in Human Synovial Membrane in Long‐standing Rheumatoid Arthritis or Osteoarthritis^a