<i>In Vivo</i> Dynamic Nuclear Polarization Magnetic Resonance Imaging for the Evaluation of Redox-Related Diseases and Theranostics

Hyodo, Fuminori; Eto, Hiroyuki; Naganuma, Tatsuya; Koyasu, Norikazu; Elhelaly, Abdelazim Elsayed; Noda, Yoshifumi; Kato, Hiroki; Murata, Masaru; Akahoshi, Tomohiko; Hashizume, Makoto; Utsumi, Hideo

doi:10.1089/ars.2021.0087

Cited by 8 publications

(4 citation statements)

References 82 publications

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“…At the selected time points (0.5, 1, 2, 4, 8, 24, and 48 h) after injection, blood and tissue samples, including the kidneys, liver, heart, and tumour, were collected and weighed. The Gd concentration in the samples was measured by ICP-MS (Hyodo et al, 2021;Jackson et al, 2015).…”

Section: In Vivo Biodistribution Studiesmentioning

confidence: 99%

MR Molecular Imaging of EGF Receptor-Overexpressing Tumours with Peptide-Targeted Gadolinium-Based Contrast Agents

Fan,

Liu,

yao

et al. 2024

Preprint

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Epidermal growth factor receptor (EGFR) is used as a biomarker for molecular imaging of cancer. Therefore, we synthesized two new Gd-DO3A-peptide complexes with an EGFR-binding peptide (NH2-CMYIEALDKYAC-COOH; EBP). To evaluate the effects of agents on targeting EGFR-overexpressing tumours, especially for more accurate cancer diagnosis and prognosis by magnetic resonance imaging (MRI), EBP-Gd-DO3A and EBP-(Gd-DO3A)3 were prepared. For in vitro cellular Gd accumulation and MRI analysis, after incubation with EBP-Gd-DO3A, EBP-(Gd-DO3A)3 or Gadovist® (a clinical macrocyclic agent) at equimolar Gd concentrations, the EGFR-overexpressing cells showed significantly higher cellular accumulation of the Gd-containing conjugates than the non-EGFR-overexpressing cells. While the cellular Gd accumulation with Gadovist® was nearly the same in all the cells. However, the cellular Gd accumulation with EBP-Gd-DO3A and EBP-(Gd-DO3A)3 was significantly reduced in the EGFR-overexpressing cells that were mocked with an inhibitory anti-EGFR monoclonal antibody, demonstrating the involvement of the EGFR pathway in the transport of both conjugates. In vitro MRI assays revealed stronger signal enhancement in the EGFR-overexpressing cells than in the non-EGFR-overexpressing cells. For in vivo pharmacokinetics and MRI analyses, the biodistribution and MR imaging was performed in the mice bearing MDA-MB-231 (EGFR-overexpressing) or U13MG (non-EGFR-overexpressing) tumour xenografts. The biodistribution and in vivo MRI experiments indicated that the improved target-specific signal enhancement of EBP-Gd-DO3A and EBP-(Gd-DO3A)3 was due to the increased accumulation of Gd in the EGFR-overexpressing tumour cells. The conjugates enabled EGFR-specific tumour MR imaging, demonstrating the potential clinical translation of these synthesized contrast agents for EGFR-based MR molecular imaging in vivo.

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Section: In Vivo Biodistribution Studiesmentioning

confidence: 99%

MR Molecular Imaging of EGF Receptor-Overexpressing Tumours with Peptide-Targeted Gadolinium-Based Contrast Agents

Fan,

Liu,

yao

et al. 2024

Preprint

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“…Studies show assessment of tissue metabolism in redox-related conditions such as cancer, inflammation and neurological disorders. It also enables treatment monitoring as a theranostic tool [ 47 ]. 13 C are used in hyperpolarized studies, but [1- 13 C]pyruvate is most commonly used in clinical trials.…”

Section: The Physical Phenomenon Of Magnetic Resonancementioning

confidence: 99%

Multinuclear MRI in Drug Discovery

et al. 2022

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The continuous development of magnetic resonance imaging broadens the range of applications to newer areas. Using MRI, we can not only visualize, but also track pharmaceutical substances and labeled cells in both in vivo and in vitro tests. 1H is widely used in the MRI method, which is determined by its high content in the human body. The potential of the MRI method makes it an excellent tool for imaging the morphology of the examined objects, and also enables registration of changes at the level of metabolism. There are several reports in the scientific publications on the use of clinical MRI for in vitro tracking. The use of multinuclear MRI has great potential for scientific research and clinical studies. Tuning MRI scanners to the Larmor frequency of a given nucleus, allows imaging without tissue background. Heavy nuclei are components of both drugs and contrast agents and molecular complexes. The implementation of hyperpolarization techniques allows for better MRI sensitivity. The aim of this review is to present the use of multinuclear MRI for investigations in drug delivery.

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“…Specifically, studies on life sciences, disease mechanisms, and drug efficacy as well as side effects require animal models for preclinical experiments [ 2 , 3 , 4 , 5 ]. Animal magnetic resonance imaging (MRI) is a non-invasive in vivo imaging technology with a high spatial and temporal resolution, enabling real-time and repetitive imaging of rodents such as mice in medical experiments, thereby allowing us to obtain a detailed observation of disease changes and trends in animal models [ 6 , 7 ]. MRI in animals has become an attractive method for evaluating the effects of animal models, and it is widely accepted by medical researchers [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Application Value of a Novel Micro-Coil in High-Resolution Imaging of Experimental Mice Based on 3.0 T Clinical MR

Qiu,

Liu,

2024

Tomography

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The clinical magnetic resonance scanner (field strength ≤ 3.0 T) has limited efficacy in the high-resolution imaging of experimental mice. This study introduces a novel magnetic resonance micro-coil designed to enhance the signal-to-noise ratio (SNR) and contrast-to-noise ratio (CNR), thereby improving high-resolution imaging in experimental mice using clinical magnetic resonance scanners. Initially, a phantom was utilized to determine the maximum spatial resolution achievable by the novel micro-coil. Subsequently, 12 C57BL/6JGpt mice were included in this study, and the novel micro-coil was employed for their scanning. A clinical flexible coil was selected for comparative analysis. The scanning methodologies for both coils were consistent. The imaging clarity, noise, and artifacts produced by the two coils on mouse tissues and organs were subjectively evaluated, while the SNR and CNR of the brain, spinal cord, and liver were objectively measured. Differences in the images produced by the two coils were compared. The results indicated that the maximum spatial resolution of the novel micro-coil was 0.2 mm. Furthermore, the subjective evaluation of the images obtained using the novel micro-coil was superior to that of the flexible coil (p < 0.05). The SNR and CNR measurements for the brain, spinal cord, and liver using the novel micro-coil were significantly higher than those obtained with the flexible coil (p < 0.001). Our study suggests that the novel micro-coil is highly effective in enhancing the image quality of clinical magnetic resonance scanners in experimental mice.

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In Vivo Dynamic Nuclear Polarization Magnetic Resonance Imaging for the Evaluation of Redox-Related Diseases and Theranostics

Cited by 8 publications

References 82 publications

MR Molecular Imaging of EGF Receptor-Overexpressing Tumours with Peptide-Targeted Gadolinium-Based Contrast Agents

MR Molecular Imaging of EGF Receptor-Overexpressing Tumours with Peptide-Targeted Gadolinium-Based Contrast Agents

Multinuclear MRI in Drug Discovery

Application Value of a Novel Micro-Coil in High-Resolution Imaging of Experimental Mice Based on 3.0 T Clinical MR

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