In vivo synergistic anti-tumor effect of paclitaxel nanoparticles combined with radiotherapy on human cervical carcinoma

Abstract

In this study, our purpose was to explore the synergistic anti-tumor effect and mechanism of paclitaxel nanoparticles (PTX-NPs) combined with radiotherapy (RT) on human cervical carcinoma (HeLa). PTX-NPs were prepared by a solid dispersion method using methoxy poly(ethylene glycol)-poly(e-caprolactone) (MPEG-PCL), which combined with RT exerted a potent and high efficient effect against cervical cancer. In vivo antitumor activity of PTX-NPs combined with RT, was estimated using nude mice carrying Hela cell xenograft tumor. The results were evaluated using microfluorine-18-deoxyglucose PET/computed tomography ( 18 F-FDG PET/CT) and immunohistochemistry. The results showed that PTX-NPs possessed a more efficient effect than PTX when combined with RT (p50.05). PTX-NPs in combination with RT might inhibit cell proliferation through its action on Ki-67, and decreased micro-vessel density (MVD) associated with CD31 and vascular endothelial growth factor (VEGF). These results suggested that PTX-NPs possessed a synergistic anti-tumor effect against cervical cancer when combined with RT.

Olaparib nanoparticles potentiated radiosensitization effects on lung cancer

“…In our previous study, we demonstrated that MPEG-PCL nanoparticles were outstanding carriers for hydrophobic drugs in virtue of the hydrophilic Time (days) Body weight (g) 28 34 PEG segment being introduced into PCL backbones. [25][26][27][28] Therefore, in our study, MPEG-PCL was used as a carrier to prepare Ola-NPs.…”

“…[29][30][31] These nanoparticles had stable particle size with a higher DL when compared with other methods reported in recent studies. 25,32,33 The lyophilized nanoparticles were relatively narrowly distributed and could be stably dissolved in water to achieve the requirements for intravenous injection. Recent studies have shown that the importance of nanoparticle size will greatly affect the metabolism of nanoparticles: ,200 nm of nanoparticles will result in a longer blood circulation time, favorable stability, lower cytotoxicity, and greater absorptive capacity due to EPR effect.…”

“…Several studies have shown that 18 F-FDG PET/CT recognized a very early decrease of glucose metabolism that is associated with progression-free survival and overall survival in many cancers. 12,25,38 A higher 18 F-FDG uptake may indicate a worse therapeutic response, while a lower uptake in tumors may suggest a better outcome. In this study, 18 F-FDG uptake in mice in the Ola-NPs + RT group was the lowest compared to that in other groups, suggesting that Ola-NPs were more effective in inhibiting tumor metabolism.…”

Novel composite drug delivery system as a novel radio sensitizer for the local treatment of cervical carcinoma

“…Cervical cancer is the second most common type of cancer in females worldwide, and is associated with local invasion and recurrent tumors (Yu et al, 2017). Several studies have demonstrated that local treatment of cervical cancer may reduce parametrial infiltration and destroy micrometastases, thereby decreasing relapse and metastasis (Thomas, 1999(Thomas, , 2000.…”

Preparation, characterization, in vitro and in vivo anti-tumor effect of thalidomide nanoparticles on lung cancer

“…In aqueous media, the copolymer may form core-shell nanostructures with an inner hydrophobic core and outer hydrophilic shell due to high solubility. [23][24][25] Encapsulation of hydrophobic drugs into polymeric nanoparticles may present a hydrophilic stabilizing interface that can prolong the circulation time in vivo and may further enhance the cellular uptake. More importantly, due to enhanced permeability and retention (EPR) effects, polymeric nanoparticles may improve the anti-tumor effects by passively targeting tumors.…”