Lactobacillus plantarum 22A-3 exerts anti-allergic activity through TGF-β secretion in passive cutaneous anaphylaxis of mice

Abstract

Allergy is a global issue, however, medical intervention for allergy treatment is limited.Recent studies have focused on allergy prevention with food factors. In this study, Lactobacillus plantarum 22A-3 (LP22A3) exerted anti-allergic effect in passive cutaneous anaphylaxis (PCA) reaction and increased transforming growth factor (TGF)-β contents in blood. Increase of TGF-β contents in blood by exogenous TGF-β injection intraperitoneally decreased Evans blue release into mice ears to the same level as LP22A3 treatment in PCA reaction. LP22A3 treatment directly to RBL-2H3 cells shows no effect on β-hexosaminidase release from RBL-2H3, but inhibited its release using the Caco-2/RBL-2H3 cells co-culture system stimulated with LP22A3 from apical side. Moreover, TGF-β treatment to RBL-2H3 inhibited β-hexosaminidase release from RBL-2H3. However, β-hexosaminidase release was cancelled by TGF-β neutralizing antibody without the influence of TGF-β mRNA expression in Caco-2 cells. These results showed that LP22A3 ameliorates allergy by TGF-β secretion through the intestine.

Lactobacillus plantarum-derived postbiotics prevent Salmonella-induced neurological dysfunctions by modulating gut–brain axis in mice

“…Figure 1 displays the schematic diagram of the experimental design. After 7-day acclimatization, all 80 mice were randomly divided into 5 groups (Control, ST, LPB active + ST, LPB inactive + ST, and LPC + ST, N = 16 in each group), and intragastrically gavaged daily with 0.2 ml of MRS broth, LPB active (1 × 10 9 cfu/ml), LPB inactive (1 × 10 9 cfu/ml) and LPC, and the dose was selected according to the previous studies ( 28 , 29 ). The body weight and feed intake were calculated every 3 days.…”

“…The genera with relative abundance above 0.05% were screened. The Spearman's correlation between two genera was identified statistically significant ( 28 ). Each node indicates one genus, and each edge represents a notable correlation among the nodes.…”

Bifidobacterium animalis KV9 and Lactobacillus vaginalis FN3 alleviated β-lactoglobulin-induced allergy by modulating dendritic cells in mice

“…Evidence has shown that probiotics can exert a variety of probiotic effects by adhering to intestinal endothelial cells, producing antibacterial metabolites, competing with pathogenic microorganisms for nutrition, strengthening the epithelial barrier, changing the immune response pathway, and regulating the immune system ( 22 , 23 ). In recent studies, probiotics such as B. longum and Lactobacillus plantarum have shown antiallergic potential in both animal models ( 12 , 24 ). In the previous study of our laboratory, six probiotics capable of regulating the secretion of IFN-γ/IL-4 by peripheral blood mononuclear cells were screened out from 48 strains.…”

Lactiplantibacillus plantarum 22A-3-induced TGF-β1 secretion from intestinal epithelial cells stimulated CD103+ DC and Foxp3+ Treg differentiation and amelioration of colitis in mice

“…It has reported that pickle-derived Lactiplantibacillus plantarum 22A-3 (LP22A3) possessed an anti-inflammatory property in passive cutaneous anaphylaxis (PCA) reaction. 9 In this present study, the anti-inflammatory effects of LP22A3 was evaluated and attempted to elucidate the underlying molecular mechanism by which LP22A3 exert its anti-inflammatory effects. In vitro gut inflammatory model was used to explore the anti-inflammatory properties of LP22A3.…”