<i>Lactobacillus plantarum</i>KFY02 enhances the relieving effect of gardenoside on montmorillonite induced constipation in mice

Mu, Jianfei; Zalán, Zsolt; Hegyi, Ferenc; Takács, Krisztina; Du, Muying

doi:10.1039/c9ra10446a

Cited by 18 publications

(12 citation statements)

References 60 publications

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“…The bacterial solution was mixed with sterile artificial gastric juice (1:9 v/v), incubated at 37 °C. The survival rates at 0 and 3 h were calculated using the following formula (Mu et al, 2020): survival rate(%) 3h viable count CFU mL −1 0h viable count CFU mL −1 × 100…”

Section: Experimental Strainmentioning

confidence: 99%

“…After the bacteria were cultured with the blank MRS-THIO medium at 37 °C for 24 h as a control, the blank and inoculation media were separately loaded into 96-well plates (200 μl/well), and the OD 600nm values of different concentrations of the medium were determined. The growth efficiency was calculated as follows (Mu et al, 2020):…”

Section: Experimental Strainmentioning

confidence: 99%

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Lactobacillus fermentum ZS40 Ameliorates Inflammation in Mice With Ulcerative Colitis Induced by Dextran Sulfate Sodium

et al. 2021

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Ulcerative colitis is an inflammatory disease of the intestine caused by many reasons, and it may even develop into colon cancer. Probiotics are normal bacteria that exist in the human body and have been proven to regulate the balance of intestinal flora and alleviate inflammation. The current study aimed to study the effect of Lactobacillus fermentum ZS40 (ZS40) on dextran sulfate sodium (DSS)-induced ulcerative colitis mice. The length and weight of the colon were measured, and the histopathological morphological changes of colon tissue were observed to evaluate the effects of ZS40 on colitis. Biochemical kits, ELISA kits, real-time quantitative PCR (RT-qPCR), and western blot were also used to detect the effects of ZS40 on serum and colon tissue related oxidative indicators and pro-inflammatory and anti-inflammatory cytokines. We found that ZS40 could reduce colonic inflammatory cell infiltration and goblet cell necrosis, increase total superoxide dismutase and catalase in mouse serum, and reduce myeloperoxidase and malondialdehyde levels. ZS40 could down-regulate the level of proinflammatory cytokines and up-regulate the level of anti-inflammatory cytokines. More importantly, ZS40 down-regulated the relative expression of nuclear factor-κB p65 (NF-κBp65), IL-6, and TNF-α mRNA and protein, up-regulated the relative expression of inhibitor kapa B alpha (IκB-α). By regulating the NF-κB and MAPK pathways to down-regulated the relative expression of p38 and JNK1/2 mRNA and p38, p-p38, JNK1/2, and p-JNK1/2 proteins. Our study suggested that ZS40 may serve as a potential therapeutical strategy for ulcerative colitis.

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Section: Experimental Strainmentioning

confidence: 99%

Section: Experimental Strainmentioning

confidence: 99%

Lactobacillus fermentum ZS40 Ameliorates Inflammation in Mice With Ulcerative Colitis Induced by Dextran Sulfate Sodium

et al. 2021

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“…Somatostatin exerts an effect on inhibiting intestinal peristalsis by reducing the release of acetylcholine and gastrointestinal hormones 35 . Substance P is known to stimulate the release of acetylcholine, thereby accelerating colonic peristalsis 36 . Gas can promote gastrointestinal motility and contraction of the pyloric sphincter 37 .…”

Section: Discussionmentioning

confidence: 99%

Alleviation of loperamide‐induced constipation with sticky rice fermented huangjiu by the regulation of serum neurotransmitters and gut microbiota

et al. 2022

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BACKGROUND: Huangjiu is an important component of traditional fermented food. It is produced by cereal fermentation. Sticky rice fermented huangjiu is an abundant source of polysaccharides, oligosaccharides, proteins, amino acids, and flavor compounds (POPAF), and it has been used as a dietary supplement and pharmaceutical ingredient. The purpose of this study is to explore the alleviation of constipation using sticky rice fermented huangjiu, with the aim of providing a basis for the nutritional treatment of constipation.RESULTS: Sticky rice fermented huangjiu was more effective in the alleviation of constipation than same concentration of ethanol treatment on serum neurotransmitters, gut microbiota, and intestinal metabolites in this 17 days constipation mouse model. Compared with ethanol treatment, the administration of sticky rice fermented huangjiu to constipated mice increased gastrointestinal motility. It alleviated the decrease in motilin (27.94%), substance P (13.85%), gastrin (63.46%), 5-hydroxytryptamine (4.55%), and short-chain fatty acid (19.80%) levels, and alleviated the increase in somatostatin levels (9.54%). Furthermore, the administration of sticky rice fermented huangjiu regulated the microbiota-mediated gut ecology through alterations in the characteristic taxa.CONCLUSION: The results reveal that sticky rice fermented huangjiu may alleviate loperamide-induced constipation by the regulation of serum neurotransmitters and gut microbiota. These findings reveal that huangjiu is endowed with many functional components by cereal fermentation, and the bioactive substances in huangjiu can be separated and applied for medical treatment or diet therapy in the future.

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“…Then, amplification was performed on a StepOne Plus real-time PCR system (Thermo Fisher Scientific) with 10 μ L of SYBR green PCR master mix (Thermo Fisher Scientific), 1 μ L of upstream primer, 1 μ L of downstream primer, 1 μ L of cDNA template, and 7 μ L of DEPC. The conditions were as follows: predenaturation at 95°C for 3 min; then denaturation at 95°C for 15 s, annealing at 60°C for 30 s, and extension at 72°C for 15 s for 40 cycles in total; the final dissolution curves were completed at 95°C for 30 s, 60°C for 30 s, and 95°C for 15 s. Finally, the relative expression level of each gene was calculated by the 2 - ΔΔ CT method, where CT was the cycle threshold, and β -actin was used as an internal reference gene [ 33 ]. Table 1 shows the primer sequence information used in this study.…”

Section: Methodsmentioning

confidence: 99%

The Preventive Effect of Lactobacillus plantarum ZS62 on DSS‐Induced IBD by Regulating Oxidative Stress and the Immune Response

Pan

Ning

et al. 2021

Oxidative Medicine and Cellular Longevity

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In this study, we used DSS to establish an IBD mouse model to study the preventive effect of Lactobacillus plantarum (L. plantarum) ZS62 on IBD in the context of oxidative stress and the immune response. We assessed the mitigating effect of this strain on IBD mice by examining the length of and histopathological changes in the colon, determining the serum antioxidant index and the levels of inflammatory cytokines, as well as the mRNA and protein expression levels of relevant genes. The study results showed that L. plantarum ZS62 could inhibit colonic atrophy in IBD mice, reduce the degree of colonic damage, downregulate the serum levels of MDA, MPO, IL-1β, IL-6, IL-12, TNF-α, and IFN-γ and the relative mRNA and protein expression of IL-1β, IL-12, TNF-α, COX-2, iNOS, and NF-κB p65 in mouse colon tissues, and upregulate the serum levels of CAT, T-SOD, and IL-10 and the relative mRNA and protein expression of Cu/Zn SOD, Mn SOD, GSH-Px, CAT, IL-10, and IκB-α in colon tissues. In summary, L. plantarum ZS62 exhibited a good preventive effect on DSS-induced IBD by regulating oxidative stress and the immune response.

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Lactobacillus plantarumKFY02 enhances the relieving effect of gardenoside on montmorillonite induced constipation in mice

Abstract: Lactobacillus plantarum KFY02 (KFY02), isolated from naturally fermented milk yoghurt in Korla, Xinjiang, Northwest of China, showed gardenoside action for the intestinal regulation of constipated mice.

Cited by 18 publications

References 60 publications

Lactobacillus fermentum ZS40 Ameliorates Inflammation in Mice With Ulcerative Colitis Induced by Dextran Sulfate Sodium

Lactobacillus fermentum ZS40 Ameliorates Inflammation in Mice With Ulcerative Colitis Induced by Dextran Sulfate Sodium

Alleviation of loperamide‐induced constipation with sticky rice fermented huangjiu by the regulation of serum neurotransmitters and gut microbiota

The Preventive Effect of Lactobacillus plantarum ZS62 on DSS‐Induced IBD by Regulating Oxidative Stress and the Immune Response

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