<i>Lactobacillus reuteri</i> ingestion and IK<sub>Ca</sub> channel blockade have similar effects on rat colon motility and myenteric neurones

Wang, B.; Mao, Yu; Diorio, Caroline; Wang, Li; Huizinga, J D; Bienenstock, John; Kunze, Wolfgang

doi:10.1111/j.1365-2982.2009.01384.x

Cited by 89 publications

(106 citation statements)

References 74 publications

(190 reference statements)

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“…Ingestion of a Lactobacillus rhamnosus strain, 'JB-1', (ref. 8 and Methods) moderated the intensity of propagated neuronally dependent peristaltic motor complexes 9 . Luminal application of JB-1 in an ex vivo small intestine peristalsis preparation within minutes reduced the amplitudes of contractions 10 .…”

mentioning

confidence: 90%

“…Luminal application of JB-1 in an ex vivo small intestine peristalsis preparation within minutes reduced the amplitudes of contractions 10 . We hypothesised 10 that this acutely alters the excitability of intestinal primary afferent neurons (IPANs) because the bacteria had no effect on slow wave related contractions, which are independent of the enteric nervous system (ENS) 9,10 .…”

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confidence: 99%

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Bacteroides fragilis polysaccharide A is necessary and sufficient for acute activation of intestinal sensory neurons

et al. 2013

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Symbionts or probiotics are known to affect the nervous system. To understand the mechanisms involved, it is important to measure sensory neuron responses and identify molecules responsible for this interaction. Here we test the effects of adding Lactobacillus rhamnosus (JB-1) and Bacteroides fragilis to the epithelium while making voltage recordings from intestinal primary afferent neurons. Sensory responses are recorded within 8 s of applying JB-1 and excitability facilitated within 15 min. Bacteroides fragilis produces similar results, as does its isolated, capsular exopolysaccharide, polysaccharide A. Lipopolysaccharide-free polysaccharide A completely mimics the neuronal effects of the parent organism. Experiments with a mutant Bacteroides fragilis devoid of polysaccharide A shows that polysaccharide A is necessary and sufficient for the neuronal effects. Complex carbohydrates have not been reported before as candidates for such signalling between symbionts and the host. These observations indicate new neuronal targets and invite further study of bacterial carbohydrates as inter-kingdom signalling molecules between beneficial bacteria and sensory neurons.

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Bacteroides fragilis polysaccharide A is necessary and sufficient for acute activation of intestinal sensory neurons

et al. 2013

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“…An example is the intermediate conductance K + ion channel (IK, KCNN4) for which a gene variant has been implicated in Crohn's disease in an Australia/New Zealand cohort in which IK mRNA expression is significantly reduced (Simms et al, 2010). Decreased IK expression would limit its role in colonic anion secretion (Barmeyer et al, 2010) and positive modulation of motility (Wang et al, 2010), which would affect the microbiota environment. Since IK is also expressed in the colon and lamina propria and modulates T cell activation and cytokine production (Logsdon et al, 1997), reduced expression might impact immune function.…”

Section: Inflammatory Bowel Diseasesmentioning

confidence: 99%

The Role of Intestinal Barrier Function in Early Life in the Development of Colitis

Anderson¹,

Dalziel²,

Gopal³

et al. 2012

Colitis

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“…Administration of Lactobacillus rhamnosus increases enteric neuron excitability and modulates colonic motility in rodents. 48,49 Moreover, it has been suggested that Lactobacillus farciminis exerts antinociceptive effects by altering central sensitization. 50 McKernan and colleagues 43 51 Lactobacillus spp.…”

Section: Alterations In the M Icrobiome And Visceral Pain Responses Imentioning

confidence: 99%

Visceral Pain and Gastrointestinal Microbiome

Chichlowski

Rudolph

2015

J Neurogastroenterol Motil

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A complex set of interactions between the microbiome, gut and brain modulate responses to visceral pain. These interactions occur at the level of the gastrointestinal mucosa, and via local neural, endocrine or immune activity; as well as by the production of factors transported through the circulatory system, like bacterial metabolites or hormones. Various psychological, infectious and other stressors can disrupt this harmonious relationship and alter both the microbiome and visceral pain responses. There are critical sensitive periods that can impact visceral pain responses in adulthood. In this review we provide a brief background of the intestinal microbiome and emerging concepts of the bidirectional interactions between the microbiome, gut and brain. We also discuss recent work in animal models, and human clinical trials using prebiotics and probiotics that alter the microbiome with resultant alterations in visceral pain responses.

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Lactobacillus reuteri ingestion and IK_Ca channel blockade have similar effects on rat colon motility and myenteric neurones

Abstract: The present results are consistent with L. reuteri enhancing tonic inhibition of colon contractile activity by acting via the IK(Ca) channel current in AH cells.

Cited by 89 publications

References 74 publications

Bacteroides fragilis polysaccharide A is necessary and sufficient for acute activation of intestinal sensory neurons

Bacteroides fragilis polysaccharide A is necessary and sufficient for acute activation of intestinal sensory neurons

The Role of Intestinal Barrier Function in Early Life in the Development of Colitis

Visceral Pain and Gastrointestinal Microbiome

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Lactobacillus reuteri ingestion and IKCa channel blockade have similar effects on rat colon motility and myenteric neurones

Abstract: The present results are consistent with L. reuteri enhancing tonic inhibition of colon contractile activity by acting via the IK(Ca) channel current in AH cells.

Cited by 89 publications

References 74 publications

Bacteroides fragilis polysaccharide A is necessary and sufficient for acute activation of intestinal sensory neurons

Bacteroides fragilis polysaccharide A is necessary and sufficient for acute activation of intestinal sensory neurons

The Role of Intestinal Barrier Function in Early Life in the Development of Colitis

Visceral Pain and Gastrointestinal Microbiome

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Product

Resources

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Lactobacillus reuteri ingestion and IK_Ca channel blockade have similar effects on rat colon motility and myenteric neurones