<i>Mycobacterium microti</i>Infection in Dairy Goats, France

Michelet, Lorraine; K, de Cruz; Phalente, Yohann; Karoui, Claudine; Hénault, Sylvie; Béral, Marina; Boschiroli, ML

doi:10.3201/eid2203.151870

Cited by 17 publications

(13 citation statements)

References 8 publications

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“…Mycobacterial species causing tuberculosis in humans and animals are part of the Mycobacterium tuberculosis complex (MTBC) [1], which includes Mycobacterium microti, a microorganism initially identified in England as a pathogen of wild rodents, such as field voles (Microtus agrestis), wood mice (Apodemus sylvaticus), bank voles (Myodes glareolus) and shrews (Sorex araneus) [2][3][4][5][6][7]. This pathogen causes natural infections in a wide range of wild and domestic animals, but in recent years, an increasing number of infections have been described in pets (cats and dogs) [8][9][10][11], wildlife (wild boar and badger) [12][13][14][15][16] and livestock (goat and cattle) [17][18][19][20]. In humans, M. microti is rarely reported as a zoonotic agent; however, its full pathogenic potential has not yet been defined [11,21].…”

Section: Introductionmentioning

confidence: 99%

Mycobacterium microti at the Environment and Wildlife Interface

et al. 2021

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An unexpected high presence of Mycobacterium microti in wild boar in Northern Italy (Garda Lake) has been reported since 2003, but the factors contributing to the maintenance of this pathogen are still unclear. In this study, we investigated the presence of M. microti in wild rodents and in water and soil samples collected at wild boar aggregation areas, such as watering holes, with the aim of clarifying their role in M. microti transmission. In total, 8 out of 120 captured animals tested positive for the Mycobacterium tuberculosis complex (MTBC) as assessed by real-time PCR, and six samples were confirmed to be M. microti. A strain with a genetic profile similar to those previously isolated in wild boars in the same area was isolated from one sample. Of the 20 water and 19 mud samples, 3 and 1, respectively, tested positive for the presence of MTBC, and spacer oligotype SB0118 (vole type) was detected in one sample. Our study suggests that wild rodents, in particular Apodemus sylvaticus, Microtus sp. and Apodemus flavicollis, play roles in the maintenance of M. microti infections in wild boar through ingestion or by contact with either infected excreta or a contaminated environment, such as at animal aggregation sites.

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Section: Introductionmentioning

confidence: 99%

Mycobacterium microti at the Environment and Wildlife Interface

et al. 2021

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“…In contrast, the field vole (Microtus agrestis) is considered the maintenance host for M. microti, a role that similarly might be played by other small mammals [44,45]. This MTC member seems to be more widespread and infecting more species than previously thought, and has been recently identified in domestic and wild populations in France and northeastern Spain [46][47][48]. We did not detect M. microti, but it could also be that its prevalence is not high enough to favor the detection of infected animals, considering the limitations of our sample size and spatial scale.…”

Section: Discussionmentioning

confidence: 99%

Detection of Wood Mice (Apodemus sylvaticus) Carrying Non-Tuberculous Mycobacteria Able to Infect Cattle and Interfere with the Diagnosis of Bovine Tuberculosis

et al. 2020

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Mycobacterial infections caused by the Mycobacterium tuberculosis complex (MTC) and non-tuberculous mycobacteria (NTM) are of great medical and veterinary relevance. The aim of this research was to study whether small mammals play a role in the epidemiology of mycobacterioses. Four samplings of 100 traps were performed in each of three cattle farms with previous history of tuberculosis or NTM between 2017 and 2018. A total of 108 animals belonging to seven species were trapped, classified, and necropsied, and tissues were submitted to microbiological and molecular methods for mycobacteria identification. The wood mouse (Apodemus sylvaticus) was the most abundant species (87%). No MTC was detected but six different NTM were identified (M. intracellulare, M. avium subsp. paratuberculosis, M. gordonae, M. celatum, M. fortuitum, and a not determined Mycobacterium sp.), showing a prevalence of 6.5%. No significant association was found between mycobacteria prevalence and the analyzed factors. Although a role in the epidemiology of MTC could not be attributed to small mammals, A. sylvaticus carries NTM that could be pathogenic or interfere with the diagnosis of tuberculosis. According to our results, there is a risk of NTM transmission at the wildlife–livestock interface through potential indirect contacts between small mammals and cattle.

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“…Those early or latent stages, with infection confirmed by either immunology or bacteriological culture but with an absence of detectable pathology, are usual in badgers and have less risk of shedding greater numbers of mycobacteria than badgers in the late stages of disease [ 37 ]. We cannot exclude that other members of the MTC were present but not recovered by culture and cross-react in P22 ELISA, i.e., Mycobacterium microti infections have been reported in both wildlife [ 42 , 43 ] and domestic animals [ 44 , 45 ] in France, although the direct detection of that species on tissue samples was not included in this study. On the other hand, the relative specificity of each method with respect to the other remained much higher for isolation (97.45% vs. 78.27% for culture and P22 ELISA, respectively).…”

Section: Discussionmentioning

confidence: 99%

Spatial and Temporal Distribution of Mycobacterium tuberculosis Complex Infection in Eurasian Badger (Meles meles) and Cattle in Asturias, Spain

Vázquez

Barral

Romero

et al. 2021

Animals

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The present work investigated the prevalence, spatial distribution, and temporal distribution of tuberculosis (TB) in free-ranging Eurasian badgers (Meles meles) and cattle in Asturias (Atlantic Spain) during a 13-year follow-up. The study objective was to assess the role of badgers as a TB reservoir for cattle and other sympatric wild species in the region. Between 2008 and 2020, 673 badgers (98 trapped and 575 killed in road traffic accidents) in Asturias were necropsied, and their tissue samples were cultured for the Mycobacterium tuberculosis complex (MTC) isolation. Serum samples were tested in an in-house indirect P22 ELISA to detect antibodies against the MTC. In parallel, data on MTC isolation and single intradermal tuberculin test results were extracted for cattle that were tested and culled as part of the Spanish National Program for the Eradication of Bovine TB. A total of 27/639 badgers (4.23%) were positive for MTC based on bacterial isolation, while 160/673 badgers (23.77%) were found to be positive with the P22 ELISA. The rate of seropositivity was higher among adult badgers than subadults. Badger TB status was spatially and temporally associated with cattle TB status. Our results cannot determine the direction of possible interspecies transmission, but they are consistent with the idea that the two hosts may exert infection pressure on each other. This study highlights the importance of the wildlife monitoring of infection and disease during epidemiological interventions in order to optimize outcomes.

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Mycobacterium microtiInfection in Dairy Goats, France

Cited by 17 publications

References 8 publications

Mycobacterium microti at the Environment and Wildlife Interface

Mycobacterium microti at the Environment and Wildlife Interface

Detection of Wood Mice (Apodemus sylvaticus) Carrying Non-Tuberculous Mycobacteria Able to Infect Cattle and Interfere with the Diagnosis of Bovine Tuberculosis

Spatial and Temporal Distribution of Mycobacterium tuberculosis Complex Infection in Eurasian Badger (Meles meles) and Cattle in Asturias, Spain

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