Petunia vein banding virus: Characterization of a New Tymovirus from Petunia × hybrida

Abstract

Petunia plants from a nursery in the State of Rio Grande do Sul, Brazil, showed pronounced vein banding and contained isometric particles with diameters of approximately 45 and 30 nm. The larger ones apparently represent a caulimovirus, while the smaller ones, which included both empty shells and full particles, were identified as those of a new tymovirus for which we propose the name Petunia vein banding virus (PetVBV). Originally, PetVBV was transmitted only with difficulty to healthy petunia plants. However, from an experimentally infected petu-nia, it was later readily transmitted also to Nicotiana benthamiana and Nicandra physalodes, but not to other species in the Solanaceae or other plant families. It produces cytopathic effects typical for tymovirus infections. Its coat protein shows approximately 65% amino acid sequence identity with those of Eggplant mosaic and Andean potato latent viruses, to which it is also serologically more closely related than to any other tymoviruses.

Genomic and biological characterization of chiltepín yellow mosaic virus, a new tymovirus infecting Capsicum annuum var. aviculare in Mexico

“…The experimental host range of ChiYMV does not correspond to those reported for any other known Tymovirus [35,36]. As most tymoviruses, ChiYMV has a narrow experimental host range, with a higher susceptibility of species from the family of the natural host [36][37][38].…”

Complete nucleotide sequence and experimental host range of Okra mosaic virus

“…We did not do any intense testing of symptomless leaves, but OkMV might still be present in these leaves and the virus titre might be reduced most probably by a plant immune response like RNA-silencing. We cannot exclude the possibility that environmental conditions are the reason for the asymptomatic leaves as it was shown for petunia vein banding virus (PetVBV) that symptoms on N. benthamiana and Nicandra physalodes were only observed during autumn and winter [49]. We did not detect any clear OkMV-induced chlorotic local [47] or systemic [7,11] lesions on C. quinoa as was reported earlier for OkMV-N, and also any persistence of symptoms in this plant species as was reported for OkMV-IC [5,11].…”

Characterization of a Strain of Tobacco Mosaic Virus from Petunia

“…In addition to the 16 viruses reported by Lesemann (1996) and three other unclassified viruses (Rubio‐Huertos and Rossel, 1959; Rubio‐Huertos, 1959; Najvi and Mahmood, 1976), Potato virus X (Sanchez‐Cuevas and Nameth, 1996) and Petunia flower mottle virus (Feldhoff et al., 1998) were also described. A double infection caused by a putative Caulimovirus and a Tymovirus has also been report in Brazil (Alexandre et al., 2000).…”