Sauromatum guttatum extract promotes wound healing and tissue regeneration in a burn mouse model via up-regulation of growth factors

Abstract

Contexts: Sauromatum guttatum (Wall.) Schott (Araceae) has been traditionally used for the treatment of wounds.Objectives: This study evaluates the healing and tissue regeneration potential of S. guttatum extract in burn wounds.Materials and methods: S. guttatum extract was analysed using various chemical tests, thin layer chromatography (TLC) and high-performance liquid chromatography (HPLC). Moreover, the extract was tested against burn associated bacteria and minimum inhibitory concentration (MIC) was also calculated. Wound healing and tissue regeneration potential was assessed using a thermally induced burn BALBc mouse model. S. guttatum extract (2% w/w) prepared in petroleum jelly, vehicle and positive control [silver sulfadiazine (SD)] groups was applied three times a day. The treatment was continued for 15 d and wound closure was measured and photographed on day 5, 10 and 15. The burnt tissues excised from wounds were subjected to histological and comparative gene expression analysis.Results: The results of the chemical tests indicated the presence of alkaloids, saponins, phenols, phytosterols, tannins, and flavonoids, while TLC and HPLC analysis indicated the presence of various compounds. The extract showed excellent activity against the tested pathogens. The lowest MIC (125 µg/mL) was observed against Staphylococcus aureus. A considerable decrease in wound area (72%) was observed in extract-treated group. Histological examination of extract-treated group showed good signs of wound healing with complete re-epithelialization and better tissue regeneration. Comparative gene expression analysis revealed the up-regulation of wound healing related PDGF, EGF and FGF genes.Conclusions: S. guttatum extract may be used to isolate bioactive constituents for the treatment of burn wounds.

Burn wound healing effects of the root hydroethanolic extract of Cochlospermum planchonii in mice

“…Celosia argentea extract ointment (10%) for example, induced early wound closure in the rats when compared with control group after 21 days (P < 0.001) (Guo et al, 2016). Sauromatum guttatum extract (2% w/w), in another study, decreased by 72% the rate of wound area with topical ointment prepared in petroleum jelly (Said et al, 2019).…”

“…Wounds can be defined as injuries occurring in living tissues that are usually caused by a cut, blow, burn or other impact, typically one in which the skin is cut or broken. Preheated materials applied directly on the skin are usually used to induce this type of wound (Cai et al, 2014;Said et al, 2019;Seyhan, 2020). The restoration of the disturbed functional status of the skin and disrupted anatomical continuity requires proper wound healing.…”

“…Worldwide, burns are responsible for more than 300.000 deaths annually, including 95.000 children less than 20 years of age with disparities 11 times higher in low-income countries (Peden et al, 2008). These data are most likely underestimated because underdeveloped countries do not have accurate statistics.…”

“…For a while, several studies have been interested in studying the proprieties of plants to heal burn wounds. Among these researches, those carried out on Buchholzia coriacea (Izah et al, 2018), Elaeis guineensis (Sene et al, 2020), and Sauromatum guttatum (Said et al, 2019) can be cited. Cochlospermum planchonii is a plant that has been the subject of several scientific studies.…”

Efficacy ofLobelia alsinoidesLam ethanolic extract on a third‐degree burn: An experimental study on rats

“…The biological process of burn wound healing is complex and is influenced by several factors, such as the health and nutritional conditions of the patient and the degree and thickness of the burn on the skin 42,43. Treatment of wound ulcers with Lobelia alsinoides Lam ointment treated groups increased the rate of the healing process and enhanced the collagen deposition compared to the control ointment treatment groups, suggesting a better repair process 44. The macroscopic examination showed that Lobelia alsinoides Lam ointment could also accelerate the healing process of the burn ulcers 45.…”