<i>Streptococcus agalactiae</i> isolates from cultured fishes in Malaysia manifesting low resistance pattern towards selected antibiotics

Aisyhah, M. A S; Amal, Mohammad Noor Azmai; Zamri‐Saad, Mohd; Siti-Zahrah, A.; Nasruddin, Nurrul Shaqinah

doi:10.1111/jfd.12351

Cited by 25 publications

(15 citation statements)

References 20 publications

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“…Instead, the other isolates showed the sensitive response to imipenem (75%), gentamicin (62.5%) ciprofloxacin and nalidixic acid (50%). Gentamicin sensitive response was corresponding with farmed red tilapia in Selangor (Ali et al 2010) and in lake in Malaysia (Aisyhah et al 2015). Tilapia isolates from local wet market in Penang showed susceptible to ciprofloxacin and trimethoprim-sulphamethoxazole and resistant to tetracycline (Budiati et al 2013).…”

Section: Resultsmentioning

confidence: 99%

“…is considered a diverse group of bacteria that possess the capacity to infect a wide range of hosts (Amal & Zamri-Saad 2011). In Malaysia, outbreaks of S. agalactiae were mostly reported in cultured red hybrid tilapia, O. niloticus × O. mossambicus (Peters), during hot seasons (Aisyhah et al 2015). Evans et al (2002) mention Streptococcus could be transmitted between fish farm and wild animal in same aquatic environment.…”

Section: Resultsmentioning

confidence: 99%

“…Temperature below 12.2°C is when tilapia loses their resistance to disease and subject to infections by bacteria, fungi and parasites (Parker 2011). High water temperature were caused the high prevalence of infections especially in large water bodies like reservoirs and dams (Aisyhah et al 2015). Nile tilapia is a tropical species and preferred temperature ranging from 31 to 36°C.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Antibiotic Resistance of Streptococcus agalactiae from Red Hybrid Tilapia (Oreochromis niloticus) in Local Wet Markets

Auzureen¹,

Hamdan²,

Jamaludin³

2016

Proceedings of International Seminar on Livestock Production and Veterinary Technology

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The existence of ethiology disease like Streptococcus agalactiae could be major problems in aquaculture field. In this study, investigations of red tilapia sold around local wet market were recorded. To detect the S. agalactiae in wet markets from disease potential and measure the antibiotic susceptibility test. Selected organs such as eyes, gills, heart and intestine were inoculated onto blood agar and incubated at 37°C for 24 h. Morphology of S. agalactiae were Gram-positive, translucent pin point colony, and has oxidase and catalase negative. Eight isolates of S. agalactiae were confirmed using PCR. The isolates samples were susceptible to imipenem (10 µg), gentamicin (10 µg), ciprofloxacin (5 µg) and nalidixic acid (30 µg). In contrast, they were resistance to ampicillin (10 µg), amoxicillin-clavulanic (20/10 µg), erythromycin (5 µg), neomycin (30 µg), norflaxacin (10 µg), streptomycin (10 µg) and trimethoprim-sulphamethoxazole (1.25/23.5 µg). Furthermore, the occurrence of resistant isolates is a matter of public health concern.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and Antibiotic Resistance of Streptococcus agalactiae from Red Hybrid Tilapia (Oreochromis niloticus) in Local Wet Markets

Auzureen¹,

Hamdan²,

Jamaludin³

2016

Proceedings of International Seminar on Livestock Production and Veterinary Technology

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“…Although, antibiotics kill pathogens, some of them will still remain and the residual antibiotics in the system increase selective pressure that would cause antibiotics resistance in bacteria (Cabello, 2006). Streptococcus agalactiae recently, isolated from tilapia in Malaysia indicated low level of resistance to amoxicillin/clavulanic acid and ampicillin (Aisyhah et al, 2015). However, antibiotics resistance represents a substantial global problem and could be transferred to ecosystems around the farm and spread amongst the bacteria so strict regulation of antibiotics and monitoring are required (Aisyhah et al, 2015).…”

Section: Control Of Streptococcal Diseasesmentioning

confidence: 99%

“…Streptococcus agalactiae recently, isolated from tilapia in Malaysia indicated low level of resistance to amoxicillin/clavulanic acid and ampicillin (Aisyhah et al, 2015). However, antibiotics resistance represents a substantial global problem and could be transferred to ecosystems around the farm and spread amongst the bacteria so strict regulation of antibiotics and monitoring are required (Aisyhah et al, 2015). Several vaccines against streptococcal infection have been developed to protect fish (Evans et al, 2004).…”

Section: Control Of Streptococcal Diseasesmentioning

confidence: 99%

A pan genome reverse vaccinology approach to prevent Streptococcus agalactiae infection in farmed tilapia

Kawasaki¹

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Streptococcus agalactiae (Group B Streptococcus; GBS) is a Gram-positive pathogenic bacterium that causes disease in a wide range of animals including fish. Outbreaks of GBS in farmed fish have substantial economic impacts on the aquaculture industry, especially in tilapia production, a staple food product in tropical and subtropical low-and middle-income countries and the second most widely farmed fish after carp. Although vaccination can effectively prevent disease caused by GBS in fish it often fails after relatively short periods of use. Capsular polysaccharide (CPS) is the major protective antigen in GBS vaccines and a critical virulence factor. There are currently ten CPS serotypes of GBS and, whilst vaccination against one serotype confers protection in fish, it is not cross-protective against other serotypes. In addition, under the strong selective pressure of vaccination-driven adaptive immunity, evolution of novel serotypes can occur. To improve the serotype crossprotective efficacy of GBS vaccines for aquaculture, I employed a pan-genome reverse vaccinology approach to design candidate serotype-independent protective vaccines based on conserved proteins in fish-pathogenic GBS serotypes. Reverse vaccinology exploits recent advances in genomics to predict antigens in the genome sequence. Reverse vaccinology combined with pan-genome can identify the antigens that are conserved across a diversity of strains of a pathogen.To identify potential vaccine candidate antigens, a pan-genome was constructed for GBS comprising 82 genomes including representative isolates from wild marine fish and stingrays, farm and companion animals and from human clinical cases in Australia, along with isolates from farmed tilapia in Honduras and representative genomes from all current serotypes available via the NCBI Genbank database. Core genome single nucleotide polymorphism 3 (SNP) analysis indicated dissemination of the aquatic host-adapted ST-261 lineage in wild Australian fish from an ancestral tilapia strain, which is congruent with several introductions of tilapia into northern Australia from Israel and Malaysia during the 1970s and 1980s.Aquatic serotype Ib GBS strains have lost many virulence factors during adaptation, but six adhesins, named ST-261 adhesins, were well-conserved across the aquatic isolates and might be critical for virulence in fish and good targets for vaccine development. Three of six ST-261 adhesins were also conserved across all strains including terrestrial isolates and were chosen as vaccine candidates. Expressions of ST-261 adhesin genes were inversely co-regulated with CPS expression dependent upon carbon source and metal ions in the medium. However, gene transcription was not reflective of surface expression as serum antibodies derived in tilapia against recombinant adhesins did not detect similar variation in the level of ST-261 adhesins in whole cell ELISA or Western blot. This may be due to posttranscriptional processing causing a disconnect between gene and protein expression.To evaluate th...

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Natural Concurrent Infection of Vibrio harveyi and V. alginolyticus in Cultured Hybrid Groupers in Malaysia

et al. 2019

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In September 2016, a marine fish farm operator in Selangor, Malaysia, reported a disease outbreak affecting juvenile hybrid groupers (Camouflage Grouper Epinephelus polyphekadion × Tiger Grouper E. fuscoguttatus). The average daily mortality was 120 fish, resulting in a cumulative mortality rate of 29% within 10 d. The affected hybrid groupers displayed lethargy, excessive mucus production, rotten fins, congestion of livers and kidneys, and enlargement of spleens. Microscopically, general congestion of the brains and internal organs was evident. Vibrio harveyi and V. alginolyticus were successfully isolated from the diseased fish. The isolated pathogens were found to be sensitive to oxytetracycline and tetracycline, but resistant towards ampicillin and vancomycin. Experimental infections using the isolated V. harveyi (10 8 CFU/mL), V. alginolyticus (10 8 CFU/mL), and concurrent infection by V. harveyi (10 8 CFU/mL) and V. alginolyticus (10 8 CFU/mL) in juvenile Asian Seabass Lates calcarifer resulted in 60, 100, and 100% mortality, respectively, within 240 h postinfection. The experimentally infected Asian Seabass demonstrated similar clinical signs and histopathological changes as the naturally infected hybrid groupers. However, concurrently infected

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Streptococcus agalactiae isolates from cultured fishes in Malaysia manifesting low resistance pattern towards selected antibiotics

Cited by 25 publications

References 20 publications

Identification and Antibiotic Resistance of Streptococcus agalactiae from Red Hybrid Tilapia (Oreochromis niloticus) in Local Wet Markets

Identification and Antibiotic Resistance of Streptococcus agalactiae from Red Hybrid Tilapia (Oreochromis niloticus) in Local Wet Markets

A pan genome reverse vaccinology approach to prevent Streptococcus agalactiae infection in farmed tilapia

Natural Concurrent Infection of Vibrio harveyi and V. alginolyticus in Cultured Hybrid Groupers in Malaysia

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