<i>Vibrio</i> responses to extracytoplasmic stress

DeAngelis, Cara; Saul-McBeth, Jessica; Matson, Jyl S.

doi:10.1111/1758-2229.12693

Cited by 15 publications

(7 citation statements)

References 82 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a separate study, insertion into or deletion of the rseB gene, produced mutants that appeared less opaque than the parental strain and, upon passaging, yielded mixed populations of opaque and translucent colonies at a high frequency [49]. The RseB protein is a periplasmic negative regulator of σ E , which controls expression of genes critical for maintaining cell envelope integrity during stress (i.e., the extracytoplasmic stress response) [50]. A strain overexpressing σ E did not recapitulate the colonial phenotype and phase-switching characteristics of rseB mutants [49].…”

Section: Cps Phase Variationmentioning

confidence: 99%

Structure, Function, and Regulation of the Essential Virulence Factor Capsular Polysaccharide of Vibrio vulnificus

Pettis

Mukerji

2020

IJMS

View full text Add to dashboard Cite

Vibrio vulnificus populates coastal waters around the world, where it exists freely or becomes concentrated in filter feeding mollusks. It also causes rapid and life-threatening sepsis and wound infections in humans. Of its many virulence factors, it is the V. vulnificus capsule, composed of capsular polysaccharide (CPS), that plays a critical role in evasion of the host innate immune system by conferring antiphagocytic ability and resistance to complement-mediated killing. CPS may also provoke a portion of the host inflammatory cytokine response to this bacterium. CPS production is biochemically and genetically diverse among strains of V. vulnificus, and the carbohydrate diversity of CPS is likely affected by horizontal gene transfer events that result in new combinations of biosynthetic genes. Phase variation between virulent encapsulated opaque colonial variants and attenuated translucent colonial variants, which have little or no CPS, is a common phenotype among strains of this species. One mechanism for generating acapsular variants likely involves homologous recombination between repeat sequences flanking the wzb phosphatase gene within the Group 1 CPS biosynthetic and transport operon. A considerable number of environmental, genetic, and regulatory factors have now been identified that affect CPS gene expression and CPS production in this pathogen.

show abstract

Section: Cps Phase Variationmentioning

confidence: 99%

Structure, Function, and Regulation of the Essential Virulence Factor Capsular Polysaccharide of Vibrio vulnificus

Pettis

Mukerji

2020

IJMS

View full text Add to dashboard Cite

show abstract

“…Members of the genus Vibrio are motile bacteria that usually existed in estuarine or marine environments. Quite a few species of Vibrio can interact with other organisms, which can be salutary symbionts or fatal pathogens ( DeAngelis, Saul-McBeth & Matson, 2018 ). In the roots of mangrove species, it is possible that Vibrios can sense and respond to various stresses to adapt to the internal environment of mangrove plants and perform their biological functions.…”

Section: Discussionmentioning

confidence: 99%

Diversity and structure of the root-associated bacterial microbiomes of four mangrove tree species, revealed by high-throughput sequencing

Sui,

He,

et al. 2023

PeerJ

View full text Add to dashboard Cite

Background Root-associated microbes of the mangrove trees play important roles in protecting and maintaining mangrove ecosystems. At present, most of our understanding of mangrove root-related microbial diversity is obtained from specific mangrove species in selected geographic regions. Relatively little is known about the composition of the bacterial microbiota existing in disparate mangrove species microenvironments, particularly the relationship among different mangrove species in tropical environments. Methods We collected the root, rhizosphere soil, and non-rhizosphere soil of four mangrove trees (Acanthus ilicifolius, Bruguiera gymnorrhiza, Clerodendrum inerme, and Lumnitzera racemosa) and detected the 16S rRNA gene by a conventional PCR. We performed high throughput sequencing using Illumina Novaseq 6000 platform (2 × 250 paired ends) to investigate the bacterial communities related with the different mangrove species. Results We analyzed the bacterial diversity and composition related to the diverse ecological niches of mangrove species. Our data confirmed distinct distribution patterns of bacterial communities in the three rhizocompartments of the four mangrove species. Microbiome composition varied with compartments and host mangrove species. The bacterial communities between the endosphere and the other two compartments were distinctly diverse independent of mangrove species. The large degree of overlap in critical community members of the same rhizocompartment across distinct mangrove species was found at the phylum level. Furthermore, this is the first report of Acidothermus found in mangrove environments. In conclusion, understanding the complicated host-microbe associations in different mangrove species could lay the foundation for the exploitation of the microbial resource and the production of secondary metabolites.

show abstract

“…Salinity and pH were indicators to assess whether Vibrio could grow well in one place. For V. cholerae disappeared in 2019, we compared the indicators of local water in these two years [22].…”

Section: Discussionmentioning

confidence: 99%

Bacterial Community Research of Migratory Bird in Chifeng, Neimeng via High-Throughput Sequencing

Zheng

Zhu

Guan

et al. 2020

Preprint

View full text Add to dashboard Cite

Background: In August 2018, a large number of migratory birds died in Chifeng, Neimeng. We were entrusted by local animal disease prevention and control center to collect the migratory bird epidemic materials and their living environmental water, and in 2019, we collect the local migratory bird stool and their living environmental water again. The bacterial communities in migratory bird epidemic materials, water and aquatic plants are profifiled by high-throughput sequencing of the V3–V4 hypervariable region of 16S rDNA gene.Results: We found that the dominant phylum between migratory bird epidemic materials, water and aquatic plant were Proteobacteia, Bacteroidetes, Firmicutes, Fusobacteria and Verrucomicrobia in 2018. One year later, we found that the dominant phylum between migratory bird stool, water and aquatic plant were Proteobacteia, Firmicutes, Cyanobacteria, Bacteroidetes. The relative abundance among bacterial phylum notably differed between two years. The relative abundance of Fusobacteria and Verrucomicrobia were higher in samples in 2018, while that of Cyanobacteria was higher in water, 2019. The relative abundance of Fusobacteria in migratory bird epidemic materials began to decline in the later period over time. At genus level, the relative abundance of Vibrio, Clostridium and other patnogenic bacteria decreased markedly and disappeared in 2019. The salt content and pH show a downward trend.Conclusions: Differences in diet and geographical location can lead to diversification in migratory bird intestinal flora. It is necessary to pay attention to diversification in intestinal flora of migratory birds, especially the abundance of vibrio in intestine. The overall structure of intestinal flora and relative abundance changes of various species are showed intuitively by 16S rDNA amplicon. But comparing to PCR which using specific primers, the accuracy and sensitivity are relatively poor. In the case of purposeful detection, it can be used in combination with both 16S rDNA amplicon and PCR which using specific primers. They are more accurately, because of supplement each other.

show abstract

Vibrio responses to extracytoplasmic stress

Cited by 15 publications

References 82 publications

Structure, Function, and Regulation of the Essential Virulence Factor Capsular Polysaccharide of Vibrio vulnificus

Structure, Function, and Regulation of the Essential Virulence Factor Capsular Polysaccharide of Vibrio vulnificus

Diversity and structure of the root-associated bacterial microbiomes of four mangrove tree species, revealed by high-throughput sequencing

Bacterial Community Research of Migratory Bird in Chifeng, Neimeng via High-Throughput Sequencing

Contact Info

Product

Resources

About