<i>Xanthomonas oryzae</i> pv. <i>oryzae</i> Exoribonuclease R Is Required for Complete Virulence in Rice, Optimal Motility, and Growth Under Stress

Roy, Sharmila; Mittal, Pragya; Tayi, Lavanya; Bondada, Sahitya; Ray, Malay K.; Patel, Hitendra Kumar; Sonti, Ramesh V.

doi:10.1094/phyto-07-21-0310-r

Cited by 4 publications

(1 citation statement)

References 64 publications

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“…It has been shown that the reduced virulence of Xoo in rice was related to its growth deficiency caused by the deletion of some genes, such as xrvC , fur, and rnr ( 23 , 34 , 35 ). However, the loss of xanfur caused no significant deficiency in the growth of Xoo in both NB liquid medium and MMX liquid medium (Fig.…”

Section: Resultsmentioning

confidence: 99%

XanFur, a novel Fur protein induced by H ₂ O ₂ , positively regulated by the global transcriptional regulator Clp and required for the full virulence of Xanthomonas oryzae pv. oryzae in rice

Zhang,

Song,

Liu

2023

Microbiol Spectr

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Bacterial leaf blight caused by Xanthomonas oryzae pv. oryzae ( Xoo ) is one of the most destructive diseases in rice. However, the pathogenic mechanisms of Xoo have not been fully understood. In this study, we identified a novel ferric uptake regulator (Fur) protein, XanFur, in Xoo, which is conserved among the major pathogens of Xanthomonas species, but has no obvious sequence identity with reported Fur proteins. Gene deletion indicated that xanfur is required for the full virulence of Xoo in rice. The expression of xanfur was significantly induced by 0.1 mM H 2 O 2 and its deletion caused a significant decrease in the tolerance of Xoo to H 2 O 2 , suggesting that xanfur is likely involved in the resistance of Xoo to the oxidative stress caused by H 2 O 2 in rice. The loss of xanfur also caused significant reduction in extracellular polysaccharide production, biofilm formation, and cell motility, suggesting that xanfur may be also involved in regulating these virulence determinants of Xoo . Furthermore, both in vivo and in vitro experimental results demonstrated that the global transcriptional regulator Clp positively regulated the expression of xanfur by directly binding to its promoter region, especially in the presence of 0.1 mM H 2 O 2 , presenting the cellular regulatory pathway for the expression of this gene. These results not only contribute to a better understanding of the interaction mechanisms between rice and Xoo , but also provide reference to developing high-effective bactericides by targeting to the Fur protein in Xoo to control bacterial leaf blight in rice. IMPORTANCE Although Xanthomonas oryzae pv. oryzae ( Xoo ) has been found to be a bacterial pathogen causing bacterial leaf blight in rice for many years, the molecular mechanisms of the rice- Xoo interaction has not been fully understood. In this study, we found that XanFur of Xoo is a novel ferric uptake regulator (Fur) protein conserved among major pathogenic Xanthomonas species. XanFur is required for the virulence of Xoo in rice, and likely involved in regulating the virulence determinants of Xoo . The expression of xanfur is induced by H 2 O 2 , and positively regulated by the global transcriptional regulator Clp. Our results reveal the function and regulation of the novel virulence-related Fur protein XanFur in Xoo, providing new insights into the interaction mechanisms of rice- Xoo .

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Section: Resultsmentioning

confidence: 99%

XanFur, a novel Fur protein induced by H ₂ O ₂ , positively regulated by the global transcriptional regulator Clp and required for the full virulence of Xanthomonas oryzae pv. oryzae in rice

Zhang,

Song,

Liu

2023

Microbiol Spectr

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RNase D Is Involved in 5S rRNA Degradation and Exopolysaccharide Production in Xanthomonas campestris pv. campestris

Chen

et al. 2023

Phytopathology®

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Ribonucleases (RNases) play critical roles in RNA metabolism and are collectively essential for cell viability. However, the most knowledge about bacterial RNases comes from the studies on Escherichia coli, very little is known about the RNases in plant pathogens. The crucifer black rot pathogen Xanthomonas campestris pv. campestris (Xcc) encodes fifteen RNases, but none of them has been functionally characterized. Here, we report the physiological function of the exoribonuclease RNase D in Xcc and provide evidences demonstrating that the Xcc RNase D is involved in 5S rRNA degradation and exopolysaccharide (EPS) production. Our work showed that the growth and virulence of Xcc were not affected by deletion of RNase D but were severely attenuated by RNase D overexpression. However, deletion of RNase D in Xcc resulted in a significant reduction in EPS production. In addition, either deletion or overexpression of RNase D in Xcc did not influence the tRNAs tested, inconsistent with the finding in E. coli that the primary function of RNase D is to participate in tRNA maturation and its overexpression degrades tRNAs. More importantly, deletion, overexpression and in vitro enzymatic analyses revealed that the Xcc RNase D degrades 5S rRNA but not 16S and 23S rRNAs that share an operon with 5S rRNA. Our results suggest that Xcc employs RNase D to realize specific modulation of the cellular 5S rRNA content after transcription and maturation whenever necessary. The finding expands our knowledge about the function of the RNase D in bacteria.

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VirB11, a traffic ATPase, mediated flagella assembly and type IV pilus morphogenesis to control the motility and virulence of Xanthomonas albilineans

Li,

Xiong,

Chen

et al. 2024

Molecular Plant Pathology

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Xanthomonas albilineans (Xal) is a gram‐negative bacterial pathogen responsible for developing sugarcane leaf scald disease, which engenders significant economic losses within the sugarcane industry. In the current study, homologous recombination exchange was carried out to induce mutations within the virB/D4‐like type IV secretion system (T4SS) genes of Xal. The results revealed that the virB11‐deletion mutant (ΔvirB11) exhibited a loss in swimming and twitching motility. Application of transmission electron microscopy analysis further demonstrated that the ΔvirB11 failed to develop flagella formation and type IV pilus morphology and exhibited reduced swarming behaviour and virulence. However, these alterations had no discernible impact on bacterial growth. Comparative transcriptome analysis between the wild‐type Xal JG43 and the deletion‐mutant ΔvirB11 revealed 123 differentially expressed genes (DEGs), of which 28 and 10 DEGs were notably associated with flagellar assembly and chemotaxis, respectively. In light of these findings, we postulate that virB11 plays an indispensable role in regulating the processes related to motility and chemotaxis in Xal.

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Xanthomonas oryzae pv. oryzae Exoribonuclease R Is Required for Complete Virulence in Rice, Optimal Motility, and Growth Under Stress

Cited by 4 publications

References 64 publications

XanFur, a novel Fur protein induced by H ₂ O ₂ , positively regulated by the global transcriptional regulator Clp and required for the full virulence of Xanthomonas oryzae pv. oryzae in rice

XanFur, a novel Fur protein induced by H ₂ O ₂ , positively regulated by the global transcriptional regulator Clp and required for the full virulence of Xanthomonas oryzae pv. oryzae in rice

RNase D Is Involved in 5S rRNA Degradation and Exopolysaccharide Production in Xanthomonas campestris pv. campestris

VirB11, a traffic ATPase, mediated flagella assembly and type IV pilus morphogenesis to control the motility and virulence of Xanthomonas albilineans

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Xanthomonas oryzae pv. oryzae Exoribonuclease R Is Required for Complete Virulence in Rice, Optimal Motility, and Growth Under Stress

Cited by 4 publications

References 64 publications

XanFur, a novel Fur protein induced by H 2 O 2 , positively regulated by the global transcriptional regulator Clp and required for the full virulence of Xanthomonas oryzae pv. oryzae in rice

XanFur, a novel Fur protein induced by H 2 O 2 , positively regulated by the global transcriptional regulator Clp and required for the full virulence of Xanthomonas oryzae pv. oryzae in rice

RNase D Is Involved in 5S rRNA Degradation and Exopolysaccharide Production in Xanthomonas campestris pv. campestris

VirB11, a traffic ATPase, mediated flagella assembly and type IV pilus morphogenesis to control the motility and virulence of Xanthomonas albilineans

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XanFur, a novel Fur protein induced by H ₂ O ₂ , positively regulated by the global transcriptional regulator Clp and required for the full virulence of Xanthomonas oryzae pv. oryzae in rice

XanFur, a novel Fur protein induced by H ₂ O ₂ , positively regulated by the global transcriptional regulator Clp and required for the full virulence of Xanthomonas oryzae pv. oryzae in rice