ICTV Virus Taxonomy Profile: Parvoviridae

Cotmore, Susan F.; Agbandje‐McKenna, Mavis; Canuti, Marta; Chiorini, John A.; Eis‐Hübinger, A. M.; Hughes, Joseph; Mietzsch, Mario; Modha, Sejal; Ogliastro, Mylène; Pénzes, Judit J.; Pintel, David J.; Qiu, Jianming; Söderlund‐Venermo, Maria; Tattersall, Peter; Tijssen, Peter

doi:10.1099/jgv.0.001212

Cited by 372 publications

(392 citation statements)

References 5 publications

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“…To investigate the genetic diversity of porcine parvoviruses, this study reconstructed the genetic relationships of seven PPV genotypes with eight assigned genera of subfamily Parvovirinae. The clustering patterns of porcine parvoviruses in this study was in line with the previous publications of which PPV1-PPV7 belonged to different genera [13,[19][20][21]. The genetic heterogeneity of porcine parvoviruses was further revealed by the fact that the virus was not only evolving at high substitution rates (10 − 3 to 10 − 5 substitutions/site/year, Fig.…”

Section: Discussionsupporting

confidence: 91%

“…According to the previous publication [19], amino acid sequences of large nonstructural protein (NS1) were used to infer the genetic relationships between parvoviruses. Aimed at phylogenetic classification and ease of topology comparison, this study included reference sequences of eight recognized genera of the subfamily Parvovirinae (Amdo-, Proto-, Ave-, Boca-, Copi-, Dependo-, Tetra-, and Erythroparvovirus) [19]. The NS1 dataset contained (i) 59 reference sequences of known genus (ii) 165 sequences downloaded from GenBank, and (iii) five sequences of Korean parvoviruses generated in this study (Additional file 1).…”

Section: Data Collection and Sequence Alignmentmentioning

confidence: 99%

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PCR-based detection and genetic characterization of porcine parvoviruses in South Korea in 2018

et al. 2020

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Background: with the advantage of sequencing technology, many novel porcine parvoviruses (PPV) rather than PPV1 has been reported. This study ultilized specific PCR-based method and gene-based analysis to study the presence and genetic diversity of porcine parvoviruses in South Korea in 2018. Results: The present study was conducted in 2018 and found PPV1 and PPV7 in nine out of 151 field samples (organs and semen) by the PCR method. Among these, the complete genome sequences of five strains (N2, N91, N108, N133, and N141) were recovered. Phylogenic analysis revealed that the strains N2, N91, and N108 belong to the PPV1 genotype, while N133 and N141 belong to PPV7 genotype. The PPV7 strains collected in this study had deletion mutations in the VP2 gene but differed from that of PPV7 strains collected in 2017. Among the PPV1 strains, the amino acid variations in the B cell epitopes of the VP2 protein were observed between three Korean PPV1 field strains (N2, N91, and N108) and the reference PPV1 strains. Those substitutions resulted in six out of 12 predicted epitopes having significant differences in antigenic index compared to the other PPV1 strains. Conclusions: This study confirmed the presence of different genotypes of porcine parvoviruses in South Korea. The PPVs circulating in South Korea were phylogenetically classified as PPV1 and PPV7 genotypes. Three Korean PPV1 strains collected in 2018 were predicted to have antigenic alteration in VP2 compared to several reference strains of PPV1.

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Section: Discussionsupporting

confidence: 91%

Section: Data Collection and Sequence Alignmentmentioning

confidence: 99%

PCR-based detection and genetic characterization of porcine parvoviruses in South Korea in 2018

et al. 2020

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“…Parvovirus genomes typically include at least two open reading frames encoding a non-structural protein and a capsid protein. The growing family Parvoviridae currently comprises 13 genera, as recommended by the International Committee on Taxonomy of Viruses (ICTV) (8). A number of recombination events have been reported in parvoviruses, such as human parvovirus B19 (9), bocavirus (10), bufavirus (11), porcine parvovirus (12), Muscovy duck parvovirus (13), goose parvovirus (14), and canine parvovirus (15).…”

Section: Introductionmentioning

confidence: 99%

Equine Parvovirus-Hepatitis in China: Characterization of Its Genetic Diversity and Evidence for Natural Recombination Events Between the Chinese and American Strains

et al. 2020

Front. Vet. Sci.

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Equine parvovirus-hepatitis (EqPV-H) was first reported in a horse that died of equine serum hepatitis in the USA in 2018, and was determined having a strong association with equine serum hepatitis in the following studies. As a newly discovered virus, the genomic sequences of only seven EqPV-H strains have been reported. Considering this, an epidemiological study was performed to investigate the prevalence of EqPV-H in equines in Guangdong Province in China, and obtain genomic sequences of the field prevalent EqPV-H strains. The detection rate of EqPV-H was finally determined to be 8.33% (95% CI: 2.8-18.4%), and EqPV-H's coinfection with equine hepacivirus and equine pegivirus was also determined. Then, the genomes of the Chinese field EqPV-H strains were obtained by PCR, sequencing, and assembly. Through bootscanning analysis, Simplot analysis, and phylogenetic analysis, strong evidence for natural recombination events were found in two Chinese field EqPV-H strains. The natural recombination events occurred between the Chinese and American strains, and were determined within VP protein. Finally, the genetic distance of EqPV-H strains was investigated. Nucleotide identities of 97.1-99.9% and 95.2-100% were found for NS and VP between EqPV-H strains, respectively. Together with other molecular evidence obtained in the present study, the genetic diversity of EqPV-H was determined. Taken together, the results of the present study expand our knowledge on the epidemiological characteristics, genetic variability, and evolution of EqPV-H.

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“…CPV2 has a small diameter (about 25 nm), enveloped with an icosahedral capsid. CPV2 is classified within the family Parvoviridae, subfamily Parvovirinae, genus Protoparvovirus, and species carnivore protoparvovirus 1 [2]. The viral genome is a single-stranded, linear, negative-sense DNA comprising about 5200 nucleotides.…”

Section: Introductionmentioning

confidence: 99%

Genotyping and phylogenetic analysis of canine parvovirus circulating in Egypt

et al. 2020

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Aim: This study aimed to detect and characterize current genotypes of canine parvovirus (CPV) in Egypt during 2018. Materials and Methods: A total of 50 fecal swabs were collected from clinically infected domestic dogs of 2-5 months of age, suspected to suffer from CPV infection, from Cairo and Giza Governorates. The samples were subjected to qualitative antigen detection using the rapid test, followed by isolation on Madin-Darby Canine Kidney (MDCK) cells, molecular characterization with partial amplification of VP2 gene using polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis. Results: Out of 50 fecal samples, 20 samples were positive (40%) by Rapid CPV/canine coronavirus Ag Test Kit. These positive samples were cultured successfully on MDCK cells. Nine randomly chosen samples out of 30 apparently negative samples were amplified using PCR with primers Hfor and Hrev to yield a typical 630 bp fragment. Then, six randomly chosen samples out of nine were amplified using PCR with primers Pbs and Pbas to yield a typical 427 bp fragment. Sequencing, BLAST analysis and assembly of the two fragments (630 bp and 427 bp) to produce 912 bp fragments, in the six samples, revealed two serotypes CPV-2b and CPV-2c. The obtained strains were submitted to GenBank and given accession numbers MK642272, MK642273, MK642274, MK642275, MK642276, and MK642277. Phylogenetic analysis of the Egyptian strains serotype 2b illustrated that they were closely related to Thailand strains (accession numbers KP715709, KP715694, KP715701, and KP715700); while Egyptian strains serotype 2c was closely related to Thailand strains (accession numbers MH711894 and MH711902), Taiwanese strain (KU244254), Chinese strain (MF467242), and Vietnamese strain (accession number LC216910). Conclusion: The current research recommends further epidemiological studies to assess the extent of the occurrence of different serotypes of CPV in Egypt and the efficiency of imported and locally produced vaccines in protection against CPV infection.

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ICTV Virus Taxonomy Profile: Parvoviridae

Cited by 372 publications

References 5 publications

PCR-based detection and genetic characterization of porcine parvoviruses in South Korea in 2018

PCR-based detection and genetic characterization of porcine parvoviruses in South Korea in 2018

Equine Parvovirus-Hepatitis in China: Characterization of Its Genetic Diversity and Evidence for Natural Recombination Events Between the Chinese and American Strains

Genotyping and phylogenetic analysis of canine parvovirus circulating in Egypt

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