IDDF2022-ABS-0260 Targeting GUT microbiota to prevent anastomotic tumors and distant metastasis in colorectal cancer surgery

Hajjar, Roy; Ajayi, Ayodeji Samuel; Oliero, Manon; Fragoso, Gabriela; Alaoui, Ahmed Amine; Cuisiniere, Thibault; Gerkins, Claire; Calvé, Annie; Rendos, Hervé Vennin; Richard, Carole; Santos, Manuela M.

doi:10.1136/gutjnl-2022-iddf.81

Cited by 3 publications

(4 citation statements)

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“…A limited number of studies have analyzed changes in the microbiota after surgical treatment for oncologic diseases (Tong et al, 2020;Chen et al, 2022;Hajjar et al, 2023). Research has shown that the microbial community after surgery is similar to that of healthy individuals, which can promote the recovery of tumor patients (Wolfe and Markey, 2022).…”

Section: Discussionmentioning

confidence: 99%

Effect of mastectomy on gut microbiota and its metabolites in patients with breast cancer

Fan,

Ding,

et al. 2024

Front. Microbiol.

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BackgroundThe relationship between gut microbiota and breast cancer has been extensively studied; however, changes in gut microbiota after breast cancer surgery are still largely unknown.Materials and methodsA total of 20 patients with breast cancer underwent routine open surgery at the First Affiliated Hospital of Hainan Medical College from 1 June 2022 to 1 December 2022. Stool samples were collected from the patients undergoing mastectomy for breast cancer preoperatively, 3 days later, and 7 days later postoperatively. The stool samples were subjected to 16s rRNA sequencing.ResultsSurgery did not affect the α-diversity of gut microbiota. The β-diversity and composition of gut microorganisms were significantly affected by surgery in breast cancer patients. Both linear discriminant analysis effect size (LEfSe) analysis and between-group differences analysis showed that surgery led to a decrease in the abundance of Firmicutes and Lachnospiraceae and an increase in the abundance of Proteobacteria and Enterobacteriaceae. Moreover, 127 differential metabolites were screened and classified into 5 categories based on their changing trends. The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed significant changes in the phenylalanine metabolic pathway and exogenous substance metabolic pathway. Eight characterized metabolites were screened using ROC analysis.ConclusionOur study found that breast cancer surgery significantly altered gut microbiota composition and metabolites, with a decrease in beneficial bacteria and an increase in potentially harmful bacteria. This underscores the importance of enhanced postoperative management to optimize gut microbiota.

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Section: Discussionmentioning

confidence: 99%

Effect of mastectomy on gut microbiota and its metabolites in patients with breast cancer

Fan,

Ding,

et al. 2024

Front. Microbiol.

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“…FMT using postoperative stool samples from AL patients leads to poor anastomotic healing in rats (Jin et al, 2022). Mice receiving preoperative stool samples from AL patients with CRC also display poor surgical healing, which is correlated with two bacterial strains, Alistipes onderdonki (A. onderdonkii) and Parabacteroides goldsteinii (P. goldsteinii), and A. onderdonkii supplementation leads to a higher rate of leaks in mice (Hajjar et al, 2022). Overall, some individual pathogens, including E. faecalis, P. aeruginosa, F. nucleatum, and A. onderdonkii, have been implicated in the pathogenesis of AL by promoting the breakdown of collagen (Figure 2).…”

Section: Anastomotic Leakmentioning

confidence: 99%

“…Although the effect of probiotics or synbiotics on the AL is not satisfactory in most clinical cases according to limited reports (Table 1), P. goldsteinii plays an anti-inflammatory effect and improves wound healing in a murine AL model (Hajjar et al, 2022), representing a potential probiotic that may enhance postoperative recovery in patients at risk of developing AL.…”

Section: Anastomotic Leakmentioning

confidence: 99%

The implication of gut microbiota in recovery from gastrointestinal surgery

Zheng

Tang

et al. 2023

Front. Cell. Infect. Microbiol.

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Recovery from gastrointestinal (GI) surgery is often interrupted by the unpredictable occurrence of postoperative complications, including infections, anastomotic leak, GI dysmotility, malabsorption, cancer development, and cancer recurrence, in which the implication of gut microbiota is beginning to emerge. Gut microbiota can be imbalanced before surgery due to the underlying disease and its treatment. The immediate preparations for GI surgery, including fasting, mechanical bowel cleaning, and antibiotic intervention, disrupt gut microbiota. Surgical removal of GI segments also perturbs gut microbiota due to GI tract reconstruction and epithelial barrier destruction. In return, the altered gut microbiota contributes to the occurrence of postoperative complications. Therefore, understanding how to balance the gut microbiota during the perioperative period is important for surgeons. We aim to overview the current knowledge to investigate the role of gut microbiota in recovery from GI surgery, focusing on the crosstalk between gut microbiota and host in the pathogenesis of postoperative complications. A comprehensive understanding of the postoperative response of the GI tract to the altered gut microbiota provides valuable cues for surgeons to preserve the beneficial functions and suppress the adverse effects of gut microbiota, which will help to enhance recovery from GI surgery.

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“…Previously, most microbiome studies focused on bacteria and were less centered on fungi, which are another important constituent of gut microbiota (Parfrey et al, 2011), including the human gut (Fujimura et al, 2016;Arrieta et al, 2018). Gut fungi play crucial roles in sustaining the homeostasis of humans by preventing pathogenic bacterial infections and protecting against intestinal complications (Hajjar et al, 2022). In addition, emerging evidence are revealing the roles of fungal microbiome in human pathology.…”

Section: Introductionmentioning

confidence: 99%

Fungal dysbiosis facilitates inflammatory bowel disease by enhancing CD4+ T cell glutaminolysis

Ding

Gong

et al. 2023

Front. Cell. Infect. Microbiol.

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The fungal microbiota is an important component of the complex multikingdom microbial community colonizing the mammalian gastrointestinal tract and has an important role in immune regulation. However, how fungi regulate inflammatory bowel disease (IBD) is poorly understood. This study found that intestinal fungi regulate immune responses in IBD. Antibiotic-mediated depletion of fungi facilitated the development of IBD. Fungi greatly enhanced oxidative phosphorylation (OXPHOS) by enhancing glutaminolysis. Mechanistically, we found that fungi could activate the dectin-1-Syk- NF-κB signaling pathway to promote the expression of key enzymes and transporters involved in glutaminolysis. In summary, our findings reveal that fungal interactions in the human gut could be a promising therapeutic target for IBD.

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IDDF2022-ABS-0260 Targeting GUT microbiota to prevent anastomotic tumors and distant metastasis in colorectal cancer surgery

Cited by 3 publications

References 0 publications

Effect of mastectomy on gut microbiota and its metabolites in patients with breast cancer

Effect of mastectomy on gut microbiota and its metabolites in patients with breast cancer

The implication of gut microbiota in recovery from gastrointestinal surgery

Fungal dysbiosis facilitates inflammatory bowel disease by enhancing CD4+ T cell glutaminolysis

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