Identificación de Salmonella Enteritidis y Salmonella Typhimurium en Cuyes mediante la Técnica de PCR Múltiple

M, Geraldine Marcelo; A, Raúl Rosadio; O, Ana Chero; O, Gerardo Díaz; C, Aldo Ciprian; Maturrano, Lenin

doi:10.15381/rivep.v28i2.13074

Cited by 8 publications

(10 citation statements)

References 3 publications

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“…Salmonella Enteritidis is one of the main causes of food-related illness worldwide, facilitated by its ability to colonize and infect a wide range of hosts, especially through eggs and products made with them 22 . In Peru, there are few studies and records related to the study of the epidemiology of this serovar, and little information related to phylogenetic and molecular studies 23,24 . Therefore, this study offers a first look at the phylogenetic structure of Peruvian S. Enteritidis strains received by INS from different regions of Peru as part of national surveillance.…”

Section: Discussionmentioning

confidence: 99%

Phylogenetic structure of Salmonella Enteritidis provides context for a foodborne outbreak in Peru

Quino

Caro-Castro

Mestanza

et al. 2020

Sci Rep

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Salmonella Enteritidis, an important foodborne zoonosis, has a dramatically increased number of cases around the world. To explore the phylogenetic structure of Peruvian Salmonella Enteritidis strains and their relationship with an outbreak occurred in 2018, we analyzed a comprehensive strains of S. Enteritidis received by the National Institute of Health during the period 2000–2018. A total of 180 strains were characterized by microbiological procedures, serotyping and whole genome sequencing. Based on genome sequences annotated, virulence factors and accessory genes were identified. Phylogenetic and population structure analysis were also analyzed based on SNPs. The phylogenetic analysis grouped the genomes into two well-supported clades that were consistent with population structure analysis. The clinical and food strains corresponding to the outbreak were included in the same cluster, which presented the sdhA gene, related to the increase of the virulence of this pathogen. The phylogenetic relationship of Peruvian S. Enteritidis suggests the presence of four S. enteritidis population with high epidemiological importance.

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Section: Discussionmentioning

confidence: 99%

Phylogenetic structure of Salmonella Enteritidis provides context for a foodborne outbreak in Peru

Quino

Caro-Castro

Mestanza

et al. 2020

Sci Rep

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“…La bacteria pudo aislarse a partir de huevos de ave, usando el método de cultivo microbiológico, luego se identificó la Salmonella mediante pruebas bioquímicas (Marcelo et al, 2017) en el laboratorio de Microbiología de la UNJBG. En el laboratorio Biotecnología de Alimentos S.A.C., a través de análisis molecular se realizó la extracción de ADN del cultivo bacteriano, mediante el método Hernandez et al (2009); luego se realizó una amplificación de 16S rDNA por PCR con el método Reysenbach et al (2000), posteriormente, se realizó una secuenciación Sanger (Fw, Rv) con el método Macrogen USA; y un análisis bioinformático con el método CodonCode Aligner NCBI nucleotide BLAST.…”

Section: Aislamiento E Identificación Molecular De Salmonella Entericaunclassified

Efecto antimicrobiano de la tunta (Solanum juzepczukii) sobre la Salmonella enterica subespecie enterica serovar Typhimurium

Castro

Mamani

2021

Rev. investig. altoandin.

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The objective of the study was to investigate the antimicrobial activity of tunta on Salmonella enterica subspecies enterica serovar Typhimurium. A natural isolate of this bacterium was used that was resistant to chloramphenicol. The experiment required the preparation of a standard solution of 0,8 g / ml of tunta extract. The Kirby Bauer technique was used for the antimicrobial evaluation of the tests and the Duraffourd scale to measure the level of sensitivity. Resulting, for the ten technical repetitions of the experimental group, an average halo of 10,40 mm and a standard deviation of 0,63 of DHI (level of limit sensitivity, according to said scale). In conclusion, our work shows evidence that tunta has an antimicrobial effect at a borderline sensitivity level on Salmonella enterica subspecies enterica serovar Typhimurium. After more in-depth research, this derivative of Solanum Jueepczukii could become an alternative for the development of antibiotics of natural origin and allow a sustainable development of Andean cultures.

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“…El serovar aislado con mayor frecuencia en cuyes (muestras de heces, linfonódulos, canal, vísceras, etc.) es S. Typhimurium (Marcelo, 2015 (Soto, 2019).…”

Section: Introductionunclassified

Resistencia antibiótica de cepas de Salmonella enterica aisladas de canales de cuyes en un mercado de Lima, 2021

Quispe

Morales-Cauti²,

Dellepiane-Gil³

et al. 2023

Rev. investig. vet. Perú

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El objetivo del presente estudio fue identificar la resistencia antibiótica de cepas aisladas de Salmonella enterica a partir de 384 muestras de canales de cuyes de un mercado de abasto en Lima, Perú. Las muestras fueron inoculadas en caldo de enriquecimiento y sembradas en medios de cultivo específicos. La identificación bacteriana se realizó a partir de pruebas bioquímicas y la resistencia antibiótica mediante el método de difusión en placa frente a 16 antibióticos. Se encontró el 28.9 ± 4.5% (111/384) de muestras positivas a cepas de Salmonella enterica. El 84.7% (94/111) mostró resistencia al menos a uno de los 16 antibióticos, mientras que 15.3% (17/111) fue sensible a todos los antibióticos. Asimismo, 40.5% (45/111) de las cepas mostró multidrogo resistencia (MDR) a tres o más antibióticos. Los resultados confirman el riesgo que representa para la salud pública la comercialización de canales de cuy contaminadas con cepas de Salmonella enterica resistentes a antibióticos.

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Identificación de Salmonella Enteritidis y Salmonella Typhimurium en Cuyes mediante la Técnica de PCR Múltiple

Cited by 8 publications

References 3 publications

Phylogenetic structure of Salmonella Enteritidis provides context for a foodborne outbreak in Peru

Phylogenetic structure of Salmonella Enteritidis provides context for a foodborne outbreak in Peru

Efecto antimicrobiano de la tunta (Solanum juzepczukii) sobre la Salmonella enterica subespecie enterica serovar Typhimurium

Resistencia antibiótica de cepas de Salmonella enterica aisladas de canales de cuyes en un mercado de Lima, 2021

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