Identification and analysis of homologues ofSaccharomyces cerevisiae Spt3 suggest conserved functional domains

Madison, Jon M.; Winston, Fred

doi:10.1002/(sici)1097-0061(19980330)14:5<409::aid-yea237>3.0.co;2-x

Yeast

1998

DOI: 10.1002/(sici)1097-0061(19980330)14:5<409::aid-yea237>3.0.co;2-x

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Identification and analysis of homologues ofSaccharomyces cerevisiae Spt3 suggest conserved functional domains

Jon M. Madison

Fred Winston

Abstract: Spt3 of Saccharomyces cerevisiae is a factor required for normal transcription from particular RNA polymerase II-dependent promoters. As a step towards analysing Spt3 structure-function relationships, we have identified and studied Spt3 homologues from three other yeasts: Kluyveromyces lactis, Clavispora opuntiae and Schizosaccharomyces pombe. Alignment of their predicted amino acid sequences shows an overall identity of 30% between all four homologues and suggests that three conserved domains are present in S… Show more

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Cited by 6 publications

(2 citation statements)

References 30 publications

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“…In addition, microarray analyses have shown that Gcn5 is involved in the regulation of a subset of genes induced during salt stress (Johnsson et al 2006). Other S. pombe orthologs of S. cerevisiae SAGA components have also been identified by sequence homology, including Spt3 (Madison and Winston 1998), Taf5 (Yamamoto et al 1997;Mitsuzawa et al 2001), Taf6 (Mitsuzawa and Ishihama 2002), and Tra1 (Hayashi et al 2007). Finally, sep9 + , which was identified in a screen for genes required for cytokinesis and sexual differentiation, was suggested to be the ortholog of S. cerevisiae SPT8 .…”

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confidence: 99%

The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8

Helmlinger¹,

Marguerat²,

Villén³

et al. 2008

Genes Dev.

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122

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The SAGA complex is a conserved multifunctional coactivator known to play broad roles in eukaryotic transcription. To gain new insights into its functions, we performed biochemical and genetic analyses of SAGA in the fission yeast, Schizosaccharomyces pombe. Purification of the S. pombe SAGA complex showed that its subunit composition is identical to that of Saccharomyces cerevisiae. Analysis of S. pombe SAGA mutants revealed that SAGA has two opposing roles regulating sexual differentiation. First, in nutrient-rich conditions, the SAGA histone acetyltransferase Gcn5 represses ste11 + , which encodes the master regulator of the mating pathway. In contrast, the SAGA subunit Spt8 is required for the induction of ste11 + upon nutrient starvation. Chromatin immunoprecipitation experiments suggest that these regulatory effects are direct, as SAGA is physically associated with the ste11 + promoter independent of nutrient levels. Genetic tests suggest that nutrient levels do cause a switch in SAGA function, as spt8⌬ suppresses gcn5⌬ with respect to ste11 + derepression in rich medium, whereas the opposite relationship, gcn5⌬ suppression of spt8⌬, occurs during starvation. Thus, SAGA plays distinct roles in the control of the switch from proliferation to differentiation in S. pombe through the dynamic and opposing activities of Gcn5 and Spt8.[Keywords: S. pombe; transcription; differentiation; SAGA; Gcn5; Ste11] Supplemental material is available at http://www.genesdev.org.

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mentioning

confidence: 99%

The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8

Helmlinger¹,

Marguerat²,

Villén³

et al. 2008

Genes Dev.

Self Cite

122

View full text Add to dashboard Cite

show abstract

“…We next analyzed the roles of Spt3p and Spt8p in the recruitment of TBP to SAGAdependent promoters. Spt3p is a functionally conserved eukaryotic transcriptional regulator (29,41) believed to play roles in both the activation and repression of transcription (4,26,40). Genetic and biochemical studies have shown that Spt3p interacts with TBP (13,14,27).…”

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confidence: 99%

Differential Requirement of SAGA Components for Recruitment of TATA-Box-Binding Protein to Promoters In Vivo

Bhaumik

Green

2002

Molecular and Cellular Biology

163

182

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The multisubunit Saccharomyces cerevisiae SAGA (Spt-Ada-Gcn5-acetyltransferase) complex is required to activate transcription of a subset of RNA polymerase II-dependent genes. However, the contribution of each SAGA component to transcription activation is relatively unknown. Here, using a formaldehyde-based in vivo cross-linking and chromatin immunoprecipitation assay, we have systematically analyzed the role of SAGA components in the recruitment of TATA-box binding protein (TBP) to SAGA-dependent promoters. We show that recruitment of TBP is diminished at a number of SAGA-dependent promoters in ada1⌬, spt7⌬, and spt20⌬ null mutants, consistent with previous biochemical data suggesting that these components maintain the integrity of the SAGA complex. We also find that Spt3p is generally required for TBP binding to SAGAdependent promoters, consistent with biochemical and genetic experiments, suggesting that Spt3p interacts with and recruits TBP to the core promoter. By contrast, Spt8p, which has been proposed to be required for the interaction between Spt3p and TBP, is required for TBP binding at only a subset of SAGA-dependent promoters. Ada2p and Ada3p are both required for TBP recruitment to Gcn5p-dependent promoters, supporting previous biochemical data that Ada2p and Ada3p are required for the histone acetyltransferase activity of Gcn5p. Finally, our results suggest that TBP-associated-factor components of SAGA are differentially required for TBP binding to SAGA-dependent promoters. In summary, we show that SAGA-dependent promoters require different combinations of SAGA components for TBP recruitment, revealing a complex combinatorial network for transcription activation in vivo.

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Characterization of a Human Homologue of theSaccharomyces cerevisiaeTranscription Factor Spt3 (SUPT3H)

Madison

Mundlos

et al. 1998

Genomics

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Identification and analysis of homologues ofSaccharomyces cerevisiae Spt3 suggest conserved functional domains

Cited by 6 publications

References 30 publications

The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8

The S. pombe SAGA complex controls the switch from proliferation to sexual differentiation through the opposing roles of its subunits Gcn5 and Spt8

Differential Requirement of SAGA Components for Recruitment of TATA-Box-Binding Protein to Promoters In Vivo

Characterization of a Human Homologue of theSaccharomyces cerevisiaeTranscription Factor Spt3 (SUPT3H)

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