Identification and characterisation of the polymorphic growth hormone gene of the Bali cattle, the Madura cattle and Ongole cattle

Abstract

A total of 150 cattle consisting of 3 breeds (Bali, Madura and Ongole) of Indonesian native cattle were used in this study. The aims of the study were to identify and characterize polymorphisms in the growth hormone loci of those breeds of cattle. The genomic DNA was extracted using Wizard genomic DNA purification system from Promega. Two fragments of growth hormone gene were amplified using PCR and continued with RFLP using restriction enzymes of AluI and MspI. Polymorphisms were found at both loci of GH-L1 and GH-L2 of the growth hormone gene by PCRRFLP analysis in all breeds of those cattle.© 2002 Jurusan Biologi FMIPA UNS SurakartaKeywords: PCR-RFLP, growth hormone gene, polymorphism, Indonesian local cattle.

Identification of Single Nucleotide Polymorphism on Growth Hormone Gene in Aceh Cattle

“…Zakizadeh et al (2006) also reported in Iranian cattle. The research of GH gene AluI in Indonesian cattle have been reported in Bali, Madura, and Benggala cattle (Sutarno et al, 2002), West Sumatra Pesisir cattle (Jakaria et al, 2007); Bali cattle (Jakaria & Noor, 2011), but the research of GH gene AluI in exon 5 in Aceh cattle have never been reported. Based on the description above, it is necessary to carry out a research to identify clearly the position of nucleotide changes of DNA sequence on GH gene exon 5 and to identify the number and SNP allele frequency on Aceh cattle.…”

Genetic diversity of Ongole Grade, Aceh, and Sumbawa cattle based on polymorphism on ND-5 fragment mitochondrial DNA using PCR-RFLP technique

“…A Haplotype forms two DNA bands measuring 336 bp and 117 bp, while B haplotype only forms one size of DNA band at 453 bp (Figure 2). This measurement referred to the size of the mitochondrial DNA sequence previously published by Anderson et al (1982) and Sutarno et al (2002). The ND-5 fragment lies in the position between bases 12058 and 12510 and has a HindIII restriction site at base position 12174.…”

“…The ND-5 fragment lies in the position between bases 12058 and 12510 and has a HindIII restriction site at base position 12174. When the ND-5 fragment is digested using HindIII restriction enzyme, two fragments will be produced consisting of 117 and 336 bp (Sutarno et al 2002) (Table 1).…”

“…Mitochondrial DNA is widely used to express genetic variation (Loftus et al 1994), because of its relatively small size, is involved in energy synthesis and has mutation rate at 5-10 times higher than core DNA (Lindberg 1989;Solihin 1994). Polymorphism of mitochondrial DNA is known to influence the phenotype (Wallace et al 1995) and the production characteristic (Lindberg 1989;Ron et al 1993;Schutz et al 1993;Sutarno et al 2002). Previous studies have shown that mitochondrial DNA affects growth, reproduction and the nature of production in livestock (Schutz et al 1993).…”

ANALYSIS ON Alu-I GROWTH HORMONE (GHAlu-I) GENE IN BALI CATTLE

“…The presence of the Alu-I restriction site corresponds to the presence of the amino acid leucine (L) at position 127 in the polypeptide chain of cattle GH, whereas the absence of this site indicates the presence of valine (V) at the same position (Lucy et al, 1991;Switonski, 2002). The study of GH gene Alu-I locus have been reported in Bavarian Simmental cattle (Schlee et al, 1994), Bali, Madura and Benggala cattle (Sutarno et al, 2002), Brahman cattle (Beauchemin et al, 2006), Angus and Shorthorn cattle (Barendse et al, 2006), Iranian cattle (Zakezadeh et al, 2006), and West Sumatra Pesisir cattle (Jakaria et al, 2007).…”