Identification and characterization of a novel carboxylesterase (FpbH) that hydrolyzes aryloxyphenoxypropionate herbicides

Wang, Chenghong; Qiu, Jiguo; Yang, Youjian; Zheng, Jufeng; He, Jun; Li, Shunpeng

doi:10.1007/s10529-016-2276-z

Cited by 22 publications

(10 citation statements)

References 17 publications

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“…CXE had a variety of physiological functions, such as participating in the degradation of nerve signal transmission pheromones and the degradation of other harmful foreign bodies in animals [ 6 ]. In plants, CXE protein plays an important role in herbicide detoxification [ 7 ], activation of hormone signal substances [ 8 ], and response to biological stress.…”

Section: Introductionmentioning

confidence: 99%

Genome-wide expression analysis of carboxylesterase (CXE) gene family implies GBCXE49 functional responding to alkaline stress in cotton

et al. 2022

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Background Carboxylesterase (CXE) is a type of hydrolase with α/β sheet hydrolase activity widely found in animals, plants and microorganisms, which plays an important role in plant growth, development and resistance to stress. Results A total of 72, 74, 39, 38 CXE genes were identified in Gossypium barbadense, Gossypium hirsutum, Gossypium raimondii and Gossypium arboreum, respectively. The gene structure and expression pattern were analyzed. The GBCXE genes were divided into 6 subgroups, and the chromosome distribution of members of the family were mapped. Analysis of promoter cis-acting elements showed that most GBCXE genes contain cis-elements related to plant hormones (GA, IAA) or abiotic stress. These 6 genes we screened out were expressed in the root, stem and leaf tissues. Combined with the heat map, GBCXE49 gene was selected for subcellular locate and confirmed that the protein was expressed in the cytoplasm. Conclusions The collinearity analysis of the CXE genes of the four cotton species in this family indicated that tandem replication played an indispensable role in the evolution of the CXE gene family. The expression patterns of GBCXE gene under different stress treatments indicated that GBCXE gene may significantly participate in the response to salt and alkaline stress through different mechanisms. Through the virus-induced gene silencing technology (VIGS), it was speculated that GBCXE49 gene was involved in the response to alkaline stress in G. barbadense.

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Section: Introductionmentioning

confidence: 99%

Genome-wide expression analysis of carboxylesterase (CXE) gene family implies GBCXE49 functional responding to alkaline stress in cotton

et al. 2022

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“…DL-2 could degrade 90.1% of QPE (50 mg/L) within 120 h [ 26 ], Aquamicrobium sp. FPB-1 could degrade 98.5% of QPE (100 mg/L) within 40 h [ 25 ]. Compared to them, YC-XJ1could degrade 96.2% of QPE (50 mg/L) within 72 h. Pseudomonas sp.…”

Section: Discussionmentioning

confidence: 99%

“…For the other reported QPE hydrolase, the specific activities of FeH from Rhodococcus ruber JPL-2 were 1.16 (U mg −1 ) for QPE with k cat / K m value of 3.25 mM −1 ·s −1 [ 48 ]; 4.12 ± 0.25 (U mg −1 ) of ChbH from Pseudomonas azotoformans QDZ-1, with k cat / K m value of 6.57 µM −1 ·s −1 [ 49 ]; 7.95 ± 0.22 (U mg −1 ) of FpbH from Aquamicrobium sp. FPB-1, with k cat / K m value of 12.85 µM −1 ·s −1 [ 25 ]. QPEH2 did not show superior degradation efficiency comparing to them.…”

Section: Discussionmentioning

confidence: 99%

The Genome Analysis of Methylobacterium populi YC-XJ1 with Diverse Xenobiotics Biodegrading Capacity and Degradation Characteristics of Related Hydrolase

Wang

Yang

et al. 2020

IJMS

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Methylobacterium populi YC-XJ1 isolated from desert soil exhibited a diverse degrading ability towards aromatic oxyphenoxypropionic acid esters (AOPPs) herbicide, phthalate esters (PAEs), organophosphorus flame retardants (OPFRs), chlorpyrifos and phoxim. The genome of YC-XJ1 was sequenced and analyzed systematically. YC-XJ1 contained a large number of exogenous compounds degradation pathways and hydrolase resources. The quizalofop-p-ethyl (QPE) degrading gene qpeh2 and diethyl phthalate (DEP) degrading gene deph1 were cloned and expressed. The characteristics of corresponding hydrolases were investigated. The specific activity of recombinant QPEH2 was 0.1 ± 0.02 U mg−1 for QPE with kcat/Km values of 1.8 ± 0.016 (mM−1·s−1). The specific activity of recombinant DEPH1 was 0.1 ± 0.02 U mg−1 for DEP with kcat/Km values of 0.8 ± 0.02 (mM−1·s−1). This work systematically illuminated the metabolic versatility of strain YC-XJ1 via the combination of genomics analysis and laboratory experiments. These results suggested that strain YC-XJ1 with diverse xenobiotics biodegrading capacity was a promising candidate for the bioremediation of polluted sites.

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“…However, it is relatively stable to breakdown using ultraviolet or sunlight and other chemical pathways [2]. In recent decades, there have been some reports on the metabolism of FPB by microbial degradation [10,11,12,13,14,15]; however, this degradation approach is not always practical in some cases, due to factors such as the inefficient efficiency of biodegradation, the greater impact of environmental conditions on the activity of the screened microorganisms, the high cost of using these microorganisms, and the long period required reach the degradation index of the herbicide residue. Therefore, it is necessary to develop innovative approaches to prevent environmental contamination of FPB from agricultural applications.…”

Section: Introductionmentioning

confidence: 99%

Nanometer Titanium Dioxide Mediated High Efficiency Photodegradation of Fluazifop-p-Butyl

Hou

Zhang

et al. 2019

IJERPH

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The widespread use of fluazifop-p-butyl (FPB) contributes to its presence in the environment. Considering the ecological risks of FPB residues in the environment, the anatase nanometer titanium dioxide (nano-TiO2) mediated photocatalytic degradation of FPB was studied by smearing FPB and nano-TiO2 together on a glass plane; illumination, trimethylsilane derivatization of photolysis products, high performance liquid chromatography (HPLC) quantitative analysis and gas chromatograph-mass spectrometer (GC-MS) identification were used. Results showed that the first order dynamic model could describe the photodegradation of FPB by nano-TiO2 mediated, and the photodegradation and photosensitization rates were found to be positively correlated with the dose of nano-TiO2 at lower dose ranges. It is noticeable that a strong photosensitization effect was exhibited on degradation of FPB, not only under high-pressure mercury lamps, but also simulated sunlight (xenon lamp light). Ultimately, twelve main photolytic products were reasonably speculated, whilst five photolysis pathways were proposed. These results together suggest that nano-TiO2 can be used as an effective photosensitizer to accelerate FPB photolysis.

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Identification and characterization of a novel carboxylesterase (FpbH) that hydrolyzes aryloxyphenoxypropionate herbicides

Abstract: FpbH is a novel AOPP herbicide-hydrolyzing carboxylesterase; it is a good candidate for mechanistic study of AOPP herbicide-hydrolyzing carboxylesterases and for bioremediation of AOPP herbicide-contaminated environments.

Cited by 22 publications

References 17 publications

Genome-wide expression analysis of carboxylesterase (CXE) gene family implies GBCXE49 functional responding to alkaline stress in cotton

Genome-wide expression analysis of carboxylesterase (CXE) gene family implies GBCXE49 functional responding to alkaline stress in cotton

The Genome Analysis of Methylobacterium populi YC-XJ1 with Diverse Xenobiotics Biodegrading Capacity and Degradation Characteristics of Related Hydrolase

Nanometer Titanium Dioxide Mediated High Efficiency Photodegradation of Fluazifop-p-Butyl

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