Identification and Characterization of a cDNA and the Structural Gene Encoding the Mouse Epithelial Membrane Protein-1

Lobsiger, Christian S.; Magyar, Josef P.; Taylor, Verdon; Wulf, Philip; Welcher, Andrew A.; Program, Amgen Est; Suter, Ueli

doi:10.1006/geno.1996.0482

Cited by 55 publications

(28 citation statements)

References 10 publications

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“…2A). In contrast, the putative extracellular domains are only poorly conserved, similar to the findings within the mammalian PMP22 family (Lobsiger et al, 1996;Taylor et al, 1995). Further inspections showed that the zebrafish protein displays the highest overall similarity to the founding member of the family, PMP22 (52% overall amino acid sequence identity to human PMP22, 48% and 50% to mouse and rat PMP22, respectively; Fig.…”

Section: Novel Zebrafish Gene Is Closely Related To Mammalian Pmp22mentioning

confidence: 61%

“…The PMP22 gene family is widely expressed in several adult tissues with the exception of MP20, which is found specifically in the lens (Kumar et al, 1993;Lobsiger et al, 1996;Taylor and Suter, 1996). The analysis of PMP22 expression in the rodent embryo showed transcripts in derivatives of all three germ layers (Baechner et al, 1995;Parmantier et al, 1997;Parmantier et al, 1995).…”

Section: Introductionmentioning

confidence: 97%

“…These proteins consist of four hydrophobic regions, and only approximately 30% of the polypeptide is thought not to be embedded in the plasma membrane (reviewed by Naef and Suter, 1998). The members of the PMP22/EMP/MP20 protein family display an amino acid identity of approximately 40% with the exception of the most distantly related MP20, which shares only about 25% identical residues (Lobsiger et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

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Characterization of peripheral myelin protein 22 in zebrafish (zPMP22) suggests an early role in the development of the peripheral nervous system

Wulf

Bernhardt²,

Suter

1999

J. Neurosci. Res.

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Peripheral myelin protein 22 (PMP22) is a component of compact myelin of the peripheral nervous system (PNS). Mutations affecting PMP22 are associated with hereditary neuropathies in humans and rodents. Although mammalian PMP22 is expressed in several tissues, the disease pathology is restricted to the PNS. We describe the characterization of a PMP22-related cDNA from zebrafish and the distribution of its cognate mRNA. Phylogenetic considerations and mRNA expression in cranial nerves are consistent with the interpretation that the encoded protein is the orthologue of mammalian PMP22. In situ hybridization analysis during development showed zebrafish PMP22 expression in embryonic sclerotome cells, in neural crest cells, and in migratory derivatives of both populations. Based on this specific expression pattern prior to the onset of myelination, we hypothesize that zebrafish PMP22 may play a role in early PNS development and that disturbance of such functions may contribute to the PNS-restricted defects caused by mutations in the mammalian PMP22 gene.

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Section: Novel Zebrafish Gene Is Closely Related To Mammalian Pmp22mentioning

confidence: 61%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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Characterization of peripheral myelin protein 22 in zebrafish (zPMP22) suggests an early role in the development of the peripheral nervous system

Wulf

Bernhardt²,

Suter

1999

J. Neurosci. Res.

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“…Several studies have shown that the EMP1 gene is expressed in a number of normal tissues (7,(18)(19)(20)(21)(22)(23). In this study, we localized and quantified for the first time EMP1 protein expression in gastric cancer tissue and normal gastric tissue using immunohistochemistry and immunoblotting.…”

Section: Discussionmentioning

confidence: 95%

Association of EMP1 with gastric carcinoma invasion, survival and prognosis

Sun

Zhao

et al. 2014

International Journal of Oncology

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Abstract. The aim of this study was to determine the expression and function of epithelial membrane protein 1 (EMP1) in gastric carcinoma. Gastric samples were taken from cancer lesions and adjacent normal tissue in gastric cancer patients immediately after endoscopic biopsy. A portion of the sample was either fixed in 4% paraformaldehyde and embedded in paraffin for immunohistochemistry or stored in liquid nitrogen for western blotting. In order to determine protein expression of EMP1 in gastric cancer (n=65) and normal tissue (n=27), semi-quantitative immunohistochemistry and western blotting were utilized. For in vitro studies, the human gastric cancer cell line SGC-7901 was maintained in RPMI-1640 medium supplemented with 10% fetal bovine serum. Recombinant lentivirus mediated overexpression of EMP1 in SGC-7901 cells was quantified with quantitative polymerase chain reaction (qPCR) and western blotting. Control SGC-7901 cells were transfected with an empty vector. To further study the effect of EMP1 overexpression in SGC-7901 cells, cell proliferation, cell apoptosis and migration and invasion assays were conducted. The expression of EMP1 was significantly lower in gastric cancer tissue compared to normal tissue using both immunohistochemistry (41.5

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“…It has been found EMP1 is a member of the PMP22 (peripheral myelin protein 22) family [25,26] . It was expressed in most tissues of the adult mouse, with highest levels in the gastrointestinal tract and lung [27] . High-level expression of EMP1 was associated with differentiation and growth arrest in squamous cells and the haematopoietic system [28] .…”

Section: Discussionmentioning

confidence: 99%

Expression patterns of esophageal cancer deregulated genes in C57BL/6J mouse embryogenesis

Zhang¹,

Gao²,

Zhi³

et al. 2004

WJG

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Identification and Characterization of a cDNA and the Structural Gene Encoding the Mouse Epithelial Membrane Protein-1

Cited by 55 publications

References 10 publications

Characterization of peripheral myelin protein 22 in zebrafish (zPMP22) suggests an early role in the development of the peripheral nervous system

Characterization of peripheral myelin protein 22 in zebrafish (zPMP22) suggests an early role in the development of the peripheral nervous system

Association of EMP1 with gastric carcinoma invasion, survival and prognosis

Expression patterns of esophageal cancer deregulated genes in C57BL/6J mouse embryogenesis

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