Identification and characterization of a type-2 diacylglycerol acyltransferase (DGAT2) from Rhodosporidium diobovatum

Chen, Zhihuan; Liu, Pengyan; Liu, Yanhua; Tang, Hui; Chen, Yunping; Zhang, Liping

doi:10.1007/s10482-014-0282-5

Cited by 8 publications

(4 citation statements)

References 30 publications

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“…The effect of substrate composition on the fatty acid profile of R. toruloides microbial oil has been highlighted also by previous studies [7,14]. Further research studies focused on proteomics and transcriptomics have demonstrated that gene expression and substrate specificity have a key role in lipid synthesis [41]. Specifically, Fillet et al mentioned that the final chain length of the fatty acid relates to the substrate preference of the elongase 3-ketoacyl-CoA [42].…”

Section: Resultsmentioning

confidence: 92%

Development of a Circular Oriented Bioprocess for Microbial Oil Production Using Diversified Mixed Confectionery Side-Streams

Tsakona

Papadaki

Kopsahelis

et al. 2019

Foods

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Diversified mixed confectionery waste streams were utilized in a two-stage bioprocess to formulate a nutrient-rich fermentation media for microbial oil production. Solid-state fermentation was conducted for the production of crude enzyme consortia to be subsequently applied in hydrolytic reactions to break down starch, disaccharides, and proteins into monosaccharides, amino acids, and peptides. Crude hydrolysates were evaluated in bioconversion processes using the red yeast Rhodosporidium toruloides DSM 4444 both in batch and fed-batch mode. Under nitrogen-limiting conditions, during fed-batch cultures, the concentration of microbial lipids reached 16.6–17 g·L−1 with the intracellular content being more than 40% (w/w) in both hydrolysates applied. R. toruloides was able to metabolize mixed carbon sources without catabolite repression. The fatty acid profile of the produced lipids was altered based on the substrate employed in the bioconversion process. Microbial lipids were rich in polyunsaturated fatty acids, with oleic acid being the major fatty acid (61.7%, w/w). This study showed that mixed food side-streams could be valorized for the production of microbial oil with high unsaturation degree, pointing towards the potential to produce tailor-made lipids for specific food applications. Likewise, the proposed process conforms unequivocally to the principles of the circular economy, as the entire quantity of confectionery by-products are implemented to generate added-value compounds that will find applications in the same original industry, thus closing the loop.

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Section: Resultsmentioning

confidence: 92%

Development of a Circular Oriented Bioprocess for Microbial Oil Production Using Diversified Mixed Confectionery Side-Streams

Tsakona

Papadaki

Kopsahelis

et al. 2019

Foods

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“…1, RtDGATa was clustered within DGAT1 family and had substantial similarity with Acyl-CoA: Cholesterol acyltransferase from S. cervisiae (ScARE). However RtDGATb was distinctly separated into DGAT2 family and had the largest similarity with RdDGAT2 from R. diobovatum, which had been proved to belong to DGAT2 family (Chen et al, 2014). Multiple sequence alignment of DGAT2 from seven organisms was performed (Fig.…”

Section: Identification and Protein Sequence Analysis Of R Toruloidementioning

confidence: 99%

“…In addition, empty vector and pYES2-DGA1 harboring DGA1 from S. cervisiae were transformed into S. cervisiae H1246 as negative (HY1) and positive controls (HY2) respectively (Table 1). Meanwhile, A RdDGAT2from other red yeast R. diobovatum which had been discussed in previous study (Chen et al, 2014) was also considered as another positive control (HY3). The results of Western blot analysis showed that all proteins were expressed in S. cervisiae H1246 and their expression levels were undifferentiated (Fig.…”

Section: R Toruloides Dgats Recovered Tag Synthesis In S Cervisiae mentioning

confidence: 99%

“…However, the molecular mechanism of TAG biosynthesis in R. toruloides had barely been studies. Recently a DGAT2 gene from another red yeast Rhodosporidium diobovatum has been cloned and its function has been confirmed in vivo by expression in the S. cerevisiae TAG-deficient quadruple mutant (Chen et al, 2014). However, not all DGAT genes have been identified in R. diobovatum because of the absence of its genome information.…”

Section: Introductionmentioning

confidence: 99%

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Identification and Characterization of Diacylglycerol Acyltransferase in Oleaginous Yeast <i>Rhodosporidium toruloides</i>

Wang¹,

Zhang²,

Zhao³

et al. 2016

American Journal of Biochemistry and Biotechnology

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Diacylglycerol acyltransferase (DGAT), which catalyzes TAG formation from DAG and acyl-CoA, has been considered to play a vital role in TAG accumulation in oleaginous microorganisms. The genome of oleaginous yeast Rhodosporidium toruloides contains two putative DGAT genes, RtDGATa and RtDGATb, which shared little conserved amino acid coding sequence with each other. Phylogeny tree analysis showed that RtDGATa belonged to DGAT1 family and RtDGATb belonged to DGAT2 family. For functional identification of the DGATs, RtDGATa and RtDGATb were individually expressed in Saccharomyces cerevisiae TAGdeficient quadruple mutant (H1246). RtDGATa had obvious preference for monounsaturated fatty acids, however the expression of RtDGATa did not alter the TAG content in S. cerevisiae H1246 and it had non-involvement in TAG accumulation according to its mRNA expression level in R. toruloides. The expression of RtDGATb could completely resume TAG biosynthesis in S. cerevisiae H1246. Substrate preference experiments revealed that RtDGATb preferred unsaturated fatty acids over saturated fatty acids, but not C18:3. Only the expression pattern of RtDGATb was related to the process of fatty acid biosynthesis, suggesting that RtDGATb plays an important role in lipid accumulation in R. toruloides.

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Exploitation of genus Rhodosporidium for microbial lipid production

Liu

2017

World J Microbiol Biotechnol

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Oleaginous microorganisms are receiving significant attention worldwide for their utility in biodiesel production and the potentiality to produce some specialty-type lipids. There is an increasing interest in isolation/adaption of robust microbe strains and design of innovative fermentation processes to make microbial lipid production a more efficient and economically feasible bio-process. Currently, the genus Rhodosporidium has been considered an important candidate, for the reason that several strains belonging to this genus have shown excellent capabilities of lipid accumulation, broad adaptabilities to various substrates, and co-production of some carotenoids. This paper reviews the current trends in the exploitation of Rhodosporidium species for microbial lipid production, including the utilization of various (single or mixed, pure or waste-derived) substrates, progress of genetic modification and metabolic engineering, innovations in fermentation mode, lipid characterizations and their potential applications. Finally, the constraints and perspectives of cultivating Rhodosporidium species for lipid production are also discussed.

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Identification and characterization of a type-2 diacylglycerol acyltransferase (DGAT2) from Rhodosporidium diobovatum

Cited by 8 publications

References 30 publications

Development of a Circular Oriented Bioprocess for Microbial Oil Production Using Diversified Mixed Confectionery Side-Streams

Development of a Circular Oriented Bioprocess for Microbial Oil Production Using Diversified Mixed Confectionery Side-Streams

Identification and Characterization of Diacylglycerol Acyltransferase in Oleaginous Yeast <i>Rhodosporidium toruloides</i>

Exploitation of genus Rhodosporidium for microbial lipid production

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