Identification and characterization of Curcuma comosa Roxb., phytoestrogens-producing plant, using AFLP markers and morphological characteristics

Keeratinijakal, Vichien; Kladmook, Maytinee; Laosatit, Kularb

doi:10.5897/jmpr09.381

Cited by 20 publications

(2 citation statements)

References 14 publications

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“…The comparison of four popular genetic markers is mentioned in Table 7. [376][377][378][379][380][381][382] .…”

Section: Amplified Fragment Length Polymorphism (Aflp)mentioning

confidence: 99%

“…A vast numbers of molecular markers are available for medicinal plant identification. The AFLP technique is commonly applied for plant classification, genetic relationships and genetic diversity in many plant species, such as Curcuma comosa, Punica granatum and Panax notoginseng 378,380,423 . Comparative studies using PCR-RFLP, RAPD and AFLP techniques have revealed that AFLP techniques are the most efficient and effective due to their high reproducibility, high quantity of information throughout multiple loci in the genome, and high resolution.…”

Section: The Comparison Of Aloe-emodin Contents Between Tlcdensitomet...mentioning

confidence: 99%

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Microscopic and molecular authentication of selected cassia species endemic to Thailand and evaluation of aloe-emodin contents in cassia garrettiana and cassia grandis leaves

Sihanat

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The genus Cassia L, belongs to the Caesalpiniaceae family, has been widely used as herbs, foods and ornamental plants for a long time. Due to the similar morphology and vernacular name, the identification of these species is perplexed. This study aimed to distinguish 16 Cassia spp. existing in Thailand using macroscopic examination, microscopic examination and AFLP fingerprinting as well as the quantitative analysis of aloe-emodin contents in C. garrettiana and C. grandis leaves collected from 15 different locations in Thailand was also developed and validated using thin-layer chromatography densitometry and thin-layer chromatography image analysis with ImageJ software. The macroscopic characteristics and transverse section of leaf through midrib were illustrated by drawing. Transverse section of leaf through midrib observed under the light microscope showed the anatomical characteristics of epidermis, palisade cells, spongy cells, vascular bundle, parenchyma and collenchyma. Leaf trichome characteristic and number observed under the light microscope and scanning electron microscopy showed the uniseriate, uni- or multicellular non-glandular and multicellular glandular trichome. However, some Cassia spp. had absent of trichome. Cassia javanica L. had the highest trichome number in both dorsal (78.94±2.86) and ventral (127.39±2.46) surfaces of the leaf whereas Cassia surattensis Burm. f. had the lowest trichome number found only on ventral (3.46±0.80) surface. The AFLP fingerprint among 16 selected Cassia spp. indicated that eleven primer combinations produced a total of distinct and reproducible 849 bands with an average 77.18 bands per primer combinations. The genetic relationship based on amplified AFLP bands showed the similarity index (SI) ranged from 0.25 to 0.78. The quantitative analysis of aloe-emodin contents from 15 sources of Cassia garrettiana Craib and Cassia grandis L. f. using TLC-densitometry with winCATS software and TLC-image analysis with ImageJ software found that C. garrettiana dried crude drug had 0.035±0.007 and 0.035±0.006 g% of aloe-emodin contents whereas in C. grandis dried crude drug had 0.412±0.067 and 0.413±0.075 g% of aloe-emodin contents, respectively. Both methods were developed and validated in term of specificity, linearity, accuracy, precision, limit of detection, limit of quantitation and robustness. The data obtained from this study provided useful information for identification of selected Thai Cassia spp. and provided the aloe-emodin contents in C. garrettiana and C. grandis leaves.

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“…The comparison of four popular genetic markers is mentioned in Table 7. [376][377][378][379][380][381][382] .…”

Section: Amplified Fragment Length Polymorphism (Aflp)mentioning

confidence: 99%

Section: The Comparison Of Aloe-emodin Contents Between Tlcdensitomet...mentioning

confidence: 99%

Microscopic and molecular authentication of selected cassia species endemic to Thailand and evaluation of aloe-emodin contents in cassia garrettiana and cassia grandis leaves

Sihanat

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Assessment of Genetic Diversity in Zingiberaceae Through Nucleotide Binding Site-Based Motif-Directed Profiling

et al. 2012

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Functional motif-directed profiling was performed with 15 nucleotide binding site (NBS) primer-enzyme combinations to identify and elucidate the phylogenetic relationships among 15 genotypes of the family Zingiberaceae. We retrieved 167 polymorphic bands (24.85 %), with an average of 11.13 bands per primer. Mean polymorphism rates were detected using MseI (26 %), RsaI (21 %), and AluI (28 %) as restriction enzymes. The polymorphism information content (PIC) for each NBS primer-enzyme combination ranged from 0.48 to 0.76 with a mean value of 0.65. The 38 NBS profiling markers had PIC values ranging from 0.3 to 0.6 and exhibited good power to discriminate between genotypes. Comparison of NBS profiling with microsatellite data for the same set of genotypes exhibited a correlation value of 0.78, P ≤ 0.001. Our study suggests that genetic variability assessment could be more efficient if it targeted genes that exhibit functionally relevant variation, rather than random markers.

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Natural triploidy in phyto-oestrogen producing Curcuma species and cultivars from Thailand

et al. 2015

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Identification and characterization of Curcuma comosa Roxb., phytoestrogens-producing plant, using AFLP markers and morphological characteristics

Cited by 20 publications

References 14 publications

Microscopic and molecular authentication of selected cassia species endemic to Thailand and evaluation of aloe-emodin contents in cassia garrettiana and cassia grandis leaves

Microscopic and molecular authentication of selected cassia species endemic to Thailand and evaluation of aloe-emodin contents in cassia garrettiana and cassia grandis leaves

Assessment of Genetic Diversity in Zingiberaceae Through Nucleotide Binding Site-Based Motif-Directed Profiling

Natural triploidy in phyto-oestrogen producing Curcuma species and cultivars from Thailand

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