Identification and Characterization of Green Microalgae, Scenedesmus sp. MCC26 and Acutodesmus obliquus MCC33 Isolated From Industrial Polluted Site Using Morphological and Molecular Markers

Abstract

Two unicellular green microalgae, were isolated from Amani Shah Nallah (Jaipur, India) that receives discharge of dyeing industries. Preliminary morphological characterization using light microscopy showed ovoid, fusiform shape of cells with single to two celled coenobia in a culture suspension. Transmission electron microscopy (TEM) analyses depict abundant chloroplast located at the periphery of the cell, a pyrenoid as well as nucleus is visible in the centre. A well developed cell wall with many layers is also evident in TEM. Phylogenetic position and genetic variabilty among the isolated microalgal strains were investigated by 18S rDNA sequence analysis. The results suggest that the isolated microalgae belongs to family chlorophyceae and corresponds to genus Scenedesmus and genus Acutodesmus. Growth profile of both microalgal strains showed a typical sigmoid curve with specific growth rate of µmax, 0.839 day-1 and 0.654 day-1 for Scenedesmus sp. and Acutodesmus obliquus, respectively. The strains were allotted with MCC numbers by IARI, New Delhi as MCC26 for Scenedesmus sp. and MCC33 for Acutodesmus obliquus.Int. J. Appl. Sci. Biotechnol. Vol 5(4): 415-422

Effect of ultra-violet light radiation on Scenedesmus vacuolatus growth kinetics, metabolic performance, and preliminary biodegradation study

“…1 a, 2 a and 3 a) respectively. Other studies have observed similar cellular morphological alterations in Scenedesmus sp after exposure to mutagen (Akgül et al 2017 ; Beherepatil and Deore 2013 ; Sarwa and Verma 2017 ). The cell elongation, cell fusion and pairing of cells could be ascribed to the effect of the UV radiation on S. vacuolatus during cellular development (Choi et al 2017 ; Colina et al 2020 ).…”

“…Moreover, the cell's morphological modification, especially the concave shape, could also improve the photosynthetic activities through improved angulation of the light ray on the cell wall. Similarly, the non-damage to the green chlorophyll pigment is an indication of the cell integrity not been damaged, this desirable since chlorophyll is a photosynthetic apparatus (Choi et al 2017 ; Sarwa and Verma 2017 ). The findings in this study were consistent with earlier studies on using UV light radiation for microalgae mutagenesis (Carino et al 2022 ; Colina et al 2020 ).…”

“…Scenedesmus belong to the class of Chlorophyceae (Akgül et al 2017). They are among the few photosynthetic microalgae that have been mass-produced for food, feed and high-value natural products globally (Sarwa and Verma 2017). It is a microalgae with unique characteristics: (a) rapid growth rate, (b) high accumulation of biomass and easy to harvest, (c) high source of natural products such as oils, pigments, protein, fatty acids and lipids, (d) it can be cultivated in low-cost medium and the presence of recalcitrant organic pollutants (e) high carbon fixation ability, (f) its ability to grow at extreme conditions such as high temperature and pH (Baghour 2019;El-Sheekh et al 2013).…”

Calcium Carbonate Mineralization of Microalgae

“…The freshwater microalgae AO was selected for the experiments, as a typical representative of photosynthetic microalgae. These microalgae strains were identified in the water bodies contaminated with industrial effluents [ 19 ]. The AO culture was obtained from the Culture Collection of Algae at Göttingen University ( Sammlung von Algenkulturen der Universität Göttingen , SAG) (Strain number 276-1).…”

Pigment production by Scenedesmus dimorphus using different low‐cost and alternative culture media

“…Classic morphological taxonomy includes the use of traits observable with light microscopy, such as arrangement of cells, appearance of chloroplasts, shape and size of cells, pattern of cells, appearance of chloroplasts and mode of division. [14][15][16] For the enrichment phase, this strain was cultured in an Erlenmeyer flask using a modified synthetic medium (BG-11) of Stanier et al 17 with the following composition: NaNO 3 1.5 g L −1 , K 2 HPO 4 0.04 g L −1 , KH 2 PO 4 0.2 g L −1 , EDTA 0.001 g L −1 , iron citrate 0.001 g L −1 , citric acid 0.006 g L −1 , Na 2 CO 3 0.02 g L −1 with 1 mL of stock metal solution per liter of BG-11 medium, composed of CO(NO 3 ) 2 0.08 g L −1 , CuSO 4 0.08 g L −1 , ZnSO 4 0.02 g L −1 , MnSO4.H2O 1.8 g L −1 , H 2 O, H 3 BO 3 2.85 g L −1 . Inoculum (10%, v/v) was added to the culture, which was left for 14 days in front of a window under natural light (mean light level was 83.72 μmol photons m −2 s −1 ).…”