Identification and Characterization of Inhibitors of Peptido-Leukotriene-Synthesis from<i>Petasites hybridus</i>

Bickel, Daniela; Röder, Thorsten; Bestmann, H. J.; Brune, K

doi:10.1055/s-2006-959492

Cited by 63 publications

(47 citation statements)

References 2 publications

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“…Some pharmacological studies have suggested that petasin and s-petasin were active ingredients in this plant [1,5,16,17], furthermore, we found that bakkenolide B (Fig. 1), a major component in the leaves of Petasites japonicus, showed significant effects in an ovalbumin-induced asthma model in our previous studies [11].…”

Section: Introductionsupporting

confidence: 62%

Optimization of Extraction Conditions for Bakkenolide B from the Leaves of Petasites japonicus by Using Response Surface Methodology

Kim¹,

Kim²,

Chung³

et al. 2014

Journal of Life Science

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Optimal conditions for extraction of bakkenolide B from Petasites japonicus leaves were determined by using response surface methodology. A second-order Box-Behnken design representing three extraction temperatures (80, 100, 120℃), three extraction times (30, 45, 60 min), and three solvent pH's (5, 7, 9) was executed. The efficiency of the extraction conditions was defined using the β-hexosamidase assay by comparing both the bakkenolide B content and its anti-allergic activity expressed as extract inhibition on degranulation. The response surface plot described for the bakkenolide B content showed that the maximum content was predicted as 121.6 µg/g with extraction conditions of 127.1℃, 46.6 min, and pH 7.7. Extraction temperature and time were important factors in determining bakkenolide B content. Using regression analysis, correlation between the inhibition effect of mast cell degranulation and bakkenolide B content was found to be low.

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Section: Introductionsupporting

confidence: 62%

Optimization of Extraction Conditions for Bakkenolide B from the Leaves of Petasites japonicus by Using Response Surface Methodology

Kim¹,

Kim²,

Chung³

et al. 2014

Journal of Life Science

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“…This assay was performed according to the literature (Bickel et al, 1994). Rat basophilic leukemia (RBL-1) cells were plated in 96-well plates at a density of 1 Â 10 5 cells/well.…”

Section: Ltc 4 Release Assaymentioning

confidence: 99%

Anti-inflammatory constituents fromPerovskia atriplicifolia

Ahmad

Waheed

Tahir

et al. 2015

Pharmaceutical Biology

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Context: Perovskia atriplicifolia Benth (Labiantae) has long been used as a traditional herbal medicine for anti-inflammation in Pakistan; this prompted us to isolate anti-inflammatory compounds from this plant. Objective: The objective of this study was to isolate and characterize the anti-inflammatory principles from Perovskia atriplicifolia. Materials and methods: The CHCl 3 -soluble fraction of the methanol extract of the whole plant on column chromatography yielded compounds 1-6. The anti-inflammatory potential of the compounds 1-6 was evaluated by Leukotriene C4 (LTC 4 ) Release Assay which was performed according to the established protocol. LTC 4 in the supernatant of each well was measured using an ELISA kit (Cayman Chemical Co., Ann Arbor, MI). Results: The bioassay-guided phytochemical investigation of the CHCl 3 soluble fraction of the methanol extract of Perovskia atriplicifolia furnished six compounds, abrotanone (1), abrotandiol (2), (+)-pinoresinol (3), (+)-syringaresinol (4), (+)-lariciresinol (5), and (+)-taxiresinol (6). The compounds (1-6) were evaluated for their inhibitory activities on LTC 4 release. Among the tested compounds, (+)-taxiresinol (6) exhibited the most potent inhibition of LTC 4 release with an IC 50 value of 3.4 ± 0.09 mM followed by compounds 4, 5, 3, and 2 with an IC 50 value ranging from 7.9 ± 0.04 to 17.2 ± 0.07 mM. Abrotanone (1) showed the lowest inhibition of LTC 4 release with an IC 50 value of 35.1 ± 0.05 mM (the positive control, zileuton, 0.77 ± 0.05 mM). Conclusion: Compounds 1-6 were found to possess inhibitory activity and seem to have potential therapeutic effect on inflammatory diseases.

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“…This assay was performed according to the literature. 33 Rat basophilic leukemia (RBL-1) cells were plated in 96-well plates at a density of 1 × 10 5 cells/well. The test compounds were added and then incubated for 10 mins.…”

Section: Methodsmentioning

confidence: 99%