Identification and Characterization of Resistance to Tobacco Etch Virus in<i>Lycopersicon</i>Species

Legnani, R.

doi:10.1094/pd-80-0306

Cited by 21 publications

(16 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The parental line M and the (M × PI24)F 1 hybrid were susceptible to PVY-N605 and TEV-CAA10, presenting coat-protein (CP) accumulation in non-inoculated tissues similar to the susceptible control (data not shown), whereas PVY and TEV-CP were never detected in tissues from the resistant line PI24. In accordance with previous studies (Légnani et al 1995(Légnani et al , 1996, these results demonstrated that PVY and TEV resistance in PI24 are inherited as recessive traits.…”

Section: Identification Of a Recessive Gene For Potyvirus Resistance supporting

confidence: 93%

“…PVY isolate N-605 (isolated from Solanum tuberosum; Jakab et al 1997) and TEV isolate CAA-10 (Légnani et al 1996) were maintained according to the Bos procedure (Bos 1969) and increased on Nicotiana tabacum cv Xanthi nc. before inoculation to tomato plants at the two-leaf stage.…”

Section: Plant Materials and Potyvirus Isolatesmentioning

confidence: 99%

“…Virus inoculum was prepared as described by Légnani et al (1995Légnani et al ( , 1996. The cotyledons and the first two leaves of tomato seedlings were mechanically inoculated.…”

Section: Potyvirus Resistance Assaymentioning

confidence: 99%

“…For both potyviruses, two independent tests on 15 plants per F 3 families were performed. Four weeks after inoculation, all plants were individually evaluated for presence/absence of PVY or TEV capsid antigen by the enzymelinked immunosorbent assay (ELISA) as described by Légnani et al (1995Légnani et al ( , 1996. Resistance evaluation of F 3 families allowed us to infer the F 2 genotypes as homozygous resistant (pot-1/pot-1), homozygous susceptible (pot-1 + /pot-1 + ) or heterozygous (pot-1/pot-1 + ).…”

Section: Potyvirus Resistance Assaymentioning

confidence: 99%

“…A complete resistance to Potato virus Y (PVY) and TEV was identified in the Lycopersicon hirsutum accession PI 247087 (Légnani et al 1995(Légnani et al , 1996. The resistance is efficient against several PVY and TEV isolates, and both temperature and inoculum pressure do not affect its expression.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Recessive resistance genes against potyviruses are localized in colinear genomic regions of the tomato (Lycopersicon spp.) and pepper (Capsicum spp.) genomes

et al. 2002

View full text Add to dashboard Cite

Resistance against both Potato virus Y (PVY) and Tobacco etch virus (TEV) was identified in the wild tomato relative Lycopersicon hirsutum PI247087. Analysis of the segregation ratio in F(2)/F(3) and BC(1) interspecific progenies indicated that a single recessive gene, or two very tightly linked recessive loci, are involved in resistance to both potyviruses. This locus was named pot-1. Using amplified fragment length polymorphism markers and a set of L. hirsutum introgression lines, pot-1 was mapped to the short arm of tomato chromosome 3, in the vicinity of the recessive py-1 locus for resistance to corky root rot. Because of the occurrence of phenotypically similar genes in pepper ( Capsicum spp.), the comparative genetics of resistance to potyviruses between tomato and pepper was investigated. Unlike most of the comparative genetic studies on resistance genes, pot-1 was tightly flanked by the same restriction fragment length polymorphism (RFLP) markers than the pvr2/pvr5 locus for resistance to PVY and TEV from pepper. These results may indicate that recessive resistance genes against potyviruses evolve less rapidly than the majority of the dominant genes cloned so far, and consequently may belong to a different family of resistance genes.

show abstract

Section: Identification Of a Recessive Gene For Potyvirus Resistance supporting

confidence: 93%

Section: Plant Materials and Potyvirus Isolatesmentioning

confidence: 99%

“…Virus inoculum was prepared as described by Légnani et al (1995Légnani et al ( , 1996. The cotyledons and the first two leaves of tomato seedlings were mechanically inoculated.…”

Section: Potyvirus Resistance Assaymentioning

confidence: 99%

Section: Potyvirus Resistance Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations