Identification and diversity of Actinomyces species in a clinical microbiology laboratory in the MALDI-TOF MS era

Fong, Peivern; Francis, Michelle J; Hamblin, John; Korman, Tony M.; Graham, Maryza

doi:10.1016/j.anaerobe.2018.09.007

Cited by 22 publications

(14 citation statements)

References 44 publications

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“…The MALDI-TOF method allows excellent, rapid and accurate identification at the genus level, but species identification remains uncertain and largely depends on the mass spectrometry systems and the available database, but improvement of this method is needed for a definitive identification of Actinomyces spp. [62][63][64]. This it will also likely facilitate the increase in knowledge about the natural habitat and therefore the relative pathogenicity of these various species [65].…”

Section: Microbiological Characteristicsmentioning

confidence: 94%

The Pathogenic Role of Actinomyces spp. and Related Organisms in Genitourinary Infections: Discoveries in the New, Modern Diagnostic Era

Gajdács

Urbán

2020

Antibiotics

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Actinomycosis is a chronic, suppurative, granulomatous infectious disease, caused by different species of Actinomyces bacteria. To date, 26 validly published Actinomyces species have been described as part of a normal human microbiota or from human clinical specimens. Due to the rapid spread of new, modern diagnostic procedures, 13 of 26 of these species have been described in this century and the Actinomycetaceae family has undergone several taxonomic revisions, including the introduction of many novel species termed Actinomyces-like organisms (ALOs). There is scarce data available on the role of these novel bacterial species in various infectious processes in human medicine. The aim of this review is to provide a comprehensive overview of Actinomyces and closely related organisms involved in human diseases—with a special focus on newly described species—in particular their role in genitourinary tract infections in females and males.

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Section: Microbiological Characteristicsmentioning

confidence: 94%

The Pathogenic Role of Actinomyces spp. and Related Organisms in Genitourinary Infections: Discoveries in the New, Modern Diagnostic Era

Gajdács

Urbán

2020

Antibiotics

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“…Using conventional microbiological tools of several attempts of samples, we could not diagnose what kind of infection in our patient. Fortunately, with the development of techniques, the Bruker MALDI-TOF BioTyper MS (MBT) and 16S rRNA gene sequencing have emerged as new reliable tools for the identi cation of Actinomyces [18,19] in recent years. And some recent studies have shown that next-generation sequence (NGS) may signi cantly enhance the rare or unusual microbiological diagnostic of the culture-negative specimens in several clinical situations by allowing rapid identi cation of bacteria in different samples, such as blood samples, sputum, body uids, or tissues [20][21][22].…”

Section: Discussionmentioning

confidence: 99%

Actinomyces as a Cause of Unexplained Fever Diagnosed by Next-Generation Sequencing: Case Report and Review

Huang¹,

Du²,

He³

et al. 2021

Preprint

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Background: Actinomycosis is a rare cause of unexplained origin fever (FUO). The NGS, particularly in cases of FUO in conventional microbiology, is a promising and advantageous technology for earlier diagnosis and more success. It gives new insights in the management of FUO, especially in infectious diseases.Case presentation: We present a case of a 71-year-old woman who had FUO that the etiology was endobronchial actinomycosis eventually diagnosed by next-generation sequencing of bronchoalveolar lavage fluid. She was admitted to the our department with intermittent fever up to 40.0℃, chills, cough with white sputum, chest distress and slightly dyspnea over one month. According to special clinical course and laboratory data of the patient, she was given intravenous sulbenicillin sodium for 11 days and oral amoxicillin at 500 mg every 8 h for another 1 month. When she returned for follow-up, she reported a notable improvement in her cough, dyspnea, sputum production and fever that was completely absent.Conclusion: In obscure causes of FUO, clinicians should consider the possibility of thoracic actinomycosis and actively use next-generation sequencing to help diagnose.

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“…MALDI-TOF MS has emerged as a rapid and effective method for bacterial identification with the ability to speciate closely related organisms. 11,12 A previous study has demonstrated the performance of MALDI-TOF MS in identification of endocarditis due to A neuii . 13 As in this case, MALDI-TOF MS was used to confirm the etiologic organism in subacute endocarditis.…”

Section: Discussionmentioning

confidence: 99%

Bacterial Endocarditis Caused by Actinomyces oris: First Reported Case and Literature Review

Phichaphop

Apiwattanakul

Wanitkun

et al. 2020

Journal of Investigative Medicine High Impact Case Reports

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Actinomyces species are gram-positive, facultative anaerobic bacilli. Infection caused by Actinomyces species is usually limited to cervicofacial, thoracic, and abdominopelvic regions. Infective endocarditis due to Actinomyces species is extremely rare with only 30 reported cases since 1939. We report a case of Actinomyces oris endocarditis in a 14-year-old boy who had a 2-week history of dyspnea on exertion without other constitutional signs. Transthoracic echocardiography was suggestive of perforation of the right coronary cusp of aortic valve. No organisms were isolated from blood cultures. The patient underwent surgical valve repair due to deteriorated cardiac function. Valve tissue culture did not initially identify the organism. However, the terminal subculture in a thioglycolate broth grew gram-positive bacilli. The matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) was compatible with Actinomyces oris. After 6 weeks of intravenous ampicillin, the patient remained well with improved cardiac function. We reviewed all reported cases of infective endocarditis caused by Actinomyces species, commenting on clinical characteristics and factors associated with unfavorable outcomes in infective endocarditis due to Actinomyces species. Although infective endocarditis caused by Actinomyces spp is rare, it could be considered in a case of culture-negative endocarditis since the clinical features might be indistinguishable from other bacterial endocarditis. Additionally, MALDI-TOF MS is a useful diagnostic tool for the identification of Actinomyces spp to improve the accuracy of diagnosis.

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Identification and diversity of Actinomyces species in a clinical microbiology laboratory in the MALDI-TOF MS era

Cited by 22 publications

References 44 publications

The Pathogenic Role of Actinomyces spp. and Related Organisms in Genitourinary Infections: Discoveries in the New, Modern Diagnostic Era

The Pathogenic Role of Actinomyces spp. and Related Organisms in Genitourinary Infections: Discoveries in the New, Modern Diagnostic Era

Actinomyces as a Cause of Unexplained Fever Diagnosed by Next-Generation Sequencing: Case Report and Review

Bacterial Endocarditis Caused by Actinomyces oris: First Reported Case and Literature Review

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