Identification and Expression Analysis of MATE Genes Involved in Flavonoid Transport in Blueberry Plants

Abstract

Multidrug and toxic compound extrusion (MATE) proteins are the most recently identified family of multidrug transporters. In plants, this family is remarkably large compared to the human and bacteria counterpart, highlighting the importance of MATE proteins in this kingdom. Here 33 Unigenes annotated as MATE transporters were found in the blueberry fruit transcriptome, of which eight full-length cDNA sequences were identified and cloned. These proteins are composed of 477–517 residues, with molecular masses ~54 kDa, and theoretical isoelectric points from 5.35 to 8.41. Bioinformatics analysis predicted 10–12 putative transmembrane segments for VcMATEs, and localization to the plasma membrane without an N-terminal signal peptide. All blueberry MATE proteins shared 32.1–84.4% identity, among which VcMATE2, VcMATE3, VcMATE5, VcMATE7, VcMATE8, and VcMATE9 were more similar to the MATE-type flavonoid transporters. Phylogenetic analysis showed VcMATE2, VcMATE3, VcMATE5, VcMATE7, VcMATE8 and VcMATE9 clustered with MATE-type flavonoid transporters, indicating that they might be involved in flavonoid transport. VcMATE1 and VcMATE4 may be involved in the transport of secondary metabolites, the detoxification of xenobiotics, or the export of toxic cations. Real-time quantitative PCR demonstrated that the expression profile of the eight VcMATE genes varied spatially and temporally. Analysis of expression and anthocyanin accumulation indicated that there were some correlation between the expression profile and the accumulation of anthocyanins. These results showed VcMATEs might be involved in diverse physiological functions, and anthocyanins across the membranes might be mutually maintained by MATE-type flavonoid transporters and other mechanisms. This study will enrich the MATE-based transport mechanisms of secondary metabolite, and provide a new biotechonology strategy to develop better nutritional blueberry cultivars.

Genome-Wide Analysis of Multidrug and Toxic Compound Extrusion (MATE) Family inGossypium raimondiiandGossypium arboreumand Its Expression Analysis Under Salt, Cadmium, and Drought Stress

“…The pI values of the predicted proteins were varied in both of the cotton genomes: in G. raimondii , the pI values ranged from 4.59 to 9.5 (for example, GrMATE39 had a pI value of 4.59, whereas GrMATE65 had a pI value of 9.5), and in G. arboreum , the pI values ranged from 5 to 9.53 (the lowest pI value was obtained for GaMATE10, with a pI value of 5, whereas GaMATE8 had the highest pI value of 9.53). The results were in agreement with previous reports on the identification and expression analysis of MATE genes in blueberry plants ( Chen et al 2015 ). WoLF PSORT was used to predict the subcellular location of the various MATE proteins.…”

“…A number of genome-wide studies and expression analyses of MATE genes have been conducted in soya bean ( Liu et al 2016 ), blueberry ( Chen et al 2015 ), Zea mays ( Zhu et al 2016 ), and other plants, but no work has been reported on diploid cotton to date, despite multiple studies on MATE gene families. Cotton is considered to be the foremost important natural fiber crop and is the textile industry’s most indispensable raw material globally ( Chakravarthy et al 2012 ; Zhou et al 2014 ).…”

The Production of Isoflavones by <em>Genista tinctoria</em> L. In Vitro Culture after Two Vanadium Compounds Application. Possible Transport Mechanism of Secondary Metabolites through Plasma Membrane

“…Despite tonoplast, participation of MATE proteins on flavonoid transport across PM has not been reported yet. In the case of Vaccinium corymbosum, several VcMATEs were identified as possible transporters due to their similarity with some known transporters from this family [62]. Therefore, decreased genistein and daidzein content in the NM could indicate MATE protein involving ( Figure 2).…”

Expression analysis of a MATE-type transporter gene ofArabidopsisand its orthologues in rice and chickpea under salt stress

“…However, in plants this family is remarkably large signifying its importance. Arabidopsis genome encodes 58 members of the MATE family and 57 in V. vinifera, 58 in Populus trichocarpa, and 38 in Zea mays and Brachypodium distachyon (Chen et al 2015). MATEs govern diverse physiological functions in the plants such as plant growth, development and stress responses, which need to be elucidated in detail (Tiwari et al 2014).…”