Identification and expression analysis of the g-type and c-type lysozymes in grass carp Ctenopharyngodon idellus

Validation of reference genes of grass carp Ctenopharyngodon idellus for the normalization of quantitative real-time PCR

“…Piscicida. Zheng et al (2007) found that upon stimulation with trivalent bacterial vaccine, LycGL mRNA levels in spleen, head kidney, and intestine of large yellow croaker Pseudosciana crocea were up-regulated, while no significant increase were observed in the liver, heart, and gills (Ye et al 2010). High expression levels of g-type lysozyme, codg1 and codg2, were observed in head kidney, spleen and peritoneum but no obvious increase was found in the liver of Atlantic cod Gadus morhua 48 h after injection (Larsen et al 2009).…”

“…Two types of lysozyme genes, g-type and c-type, had been reported in grass carp (Ye et al 2010). In this study, the expression levels of g-type gene of grass carp 9, 24, and 72 h post-infection in liver, spleen and head kidney were normalized to those of EF1a gene, and the normalized and unnormalized data were compared.…”

“…Primers for grass carp g-type lysozyme gene and plasmid for standard cure establishment were designed and constructed as previously described by Ye et al (2010). qRT-PCR and data analysis were the same as described above.…”

Characterization and expression analysis of a goose-type lysozyme from the rock bream Oplegnathus fasciatus, and antimicrobial activity of its recombinant protein

“…Even though lysozyme has been shown to play a significant role in defense against infectious disease in fish, its underlying functional mechanisms remain to be completely understood [2]. In fish, the g-type lysozyme has been identified in Japanese flounder [20], common carp [21], orange-spotted grouper [22], Chinese perch [23], large yellow croaker [24], Atlantic salmon [25], brill [26], Atlantic cod [27], grass carp [28] and rohu [29].…”

Expression of c-type lysozyme gene in sea cucumber (Apostichopus japonicus) is highly regulated and time dependent after salt stress

“…The sequence displayed several typical characteristics shared by LysC counterparts including LysC family domain profile and supported their classification under LysC subfamily. LysC subfamily from all of the known species possesses two putative conserved catalytic residues (Glu and Asp) and eight conserved cysteine residues that could form four pairs of disulfide bridges (Jimenez-Cantizano et al, 2008;Hikima et al, 1997;Ye et al, 2010). The conserved catalytic residues in AjcLYZ are important for their functions as well as other residues.…”