Identification and functional analysis of a promoter sequence for phloem tissue specific gene expression from Populus trichocarpa

Non-dispersive phloem-protein bodies (NPBs) ofPopulus trichocarpaconsist of a SEOR protein and do not respond to cell wounding and Ca2+

“…7 ), which may reflect recent gene multiplication events. The promoter region of one of the corresponding genes, Potri.001G340200.1, recently has been shown to be active specifically in phloem tissue ( Nguyen et al, 2017 ). Unfortunately, the resolution of the histological approach did not allow these authors to differentiate between the cell types of the phloem, and it remains unclear whether the promoter, denoted PtrDP3 , is sieve element-specific like the promoters of VfSEO1 ( Noll et al, 2007 ), AtSEOR1 ( Froelich et al, 2011 ), AtSEOR2 ( Rüping et al, 2010 ) and MtSEO1 ( Bucsenez et al, 2012 ).…”

“…Unfortunately, the resolution of the histological approach did not allow these authors to differentiate between the cell types of the phloem, and it remains unclear whether the promoter, denoted PtrDP3 , is sieve element-specific like the promoters of VfSEO1 ( Noll et al, 2007 ), AtSEOR1 ( Froelich et al, 2011 ), AtSEOR2 ( Rüping et al, 2010 ) and MtSEO1 ( Bucsenez et al, 2012 ). Nguyen et al (2017) determined that the sequence located within −200 to −300 bp from the translation initiation codon was sufficient to render the PtrDP3 promoter phloem-specific. An alignment of the putative promoter sequence of the PtSEOR1 gene Potri017G071000.1 and that of PtrDP3 revealed a high degree of homology between the 100-bp sequence conferring phloem-specificity to the latter and the corresponding sequence of PtSEOR1 , including a conserved TATA-box motif ( Fig.…”

“… Identical bases appear in blue, different bases and gaps are shown in red. The 100-bp sequence that confers phloem-specificity to the Potri.001G340200.1 promoter ( Nguyen et al, 2017 ) and the corres-ponding sequence in Potri.017G071000.1, the PtSEOR1 gene, are shown on yellow background. Of the 100 base pairs, 71 are conserved.…”

Biological Parts for Plant Biodesign to Enhance Land-Based Carbon Dioxide Removal

“…For example, CDR engineering involves the modification of plant form, such as changing root architecture with less nodal root number and more deep roots in maize [176], which requires precise control of gene expression by tissue-specific promoters [177]. Leaf-specific promoters [178] can be used for driving the expression of genes involved in CO 2 fixation; phloem tissuespecific promoters [179] can be used for genes involved in phloem-mediated translocation of sugars; and root-specific promoters [180,181] can be used for genes involved in root growth and development. Besides tissue-specific promoters, cell-type-specific promoters [182,183] can be used for highprecision control of the spatial expression pattern of CDRrelated genes, Also, to optimize the performance of a plant system for CDR, it is necessary to fine-tune the expression of genes involved in different processes (e.g., CO 2 fixation, carbon partitioning and translocation, and carbon storage) to achieve an optimal balance between source and sink activities.…”

Promoter PPSP1–5-BnPSP-1 From Ramie (Boehmeria nivea L. Gaud.) Can Drive Phloem-Specific GUS Expression in Arabidopsis thaliana

“…The study in tomato cytosolic fructokinase FRK1 suggest that SlFRK1 is involved in vascular tissue development and hydraulic conductivity in tomato plants and that SlFRK1 is important for normal phloem fiber development, together with SlFRK2 (Stein et al, 2017). Research on the identification of the phloem-specific activity of the poplar PtrDP3 promoter found that the transgenic Arabidopsis plants are not affected by abiotic stress or exogenously applied plant hormones (Nguyen et al, 2017). This study provides evidence of a strong phloem-specific promoter that is suitable for phloem-specific biotechnological modifications in plants.…”