Identification and molecular characteristics of verotoxin-producing Escherichia coli (VTEC) from bovine and pig carcasses isolated in Poland during 2014–2018

Wieczorek, Kinga; Osek, Jacek

doi:10.1016/j.fm.2020.103587

Cited by 7 publications

(3 citation statements)

References 55 publications

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“…However, the most prevalent serogroups were O6, O155, and O21, which were not consistent with other regions in China (Peng et al 2019). Uncommon serotypes have also been more frequently identified from ruminants in other regions (Wieczorek and Osek 2020). The increasing identification methods and high accuracy and sensitivity of WGS technology in predicting STEC serotypes may contribute to this issue.…”

Section: Discussionmentioning

confidence: 99%

Genetic and phylogenetic characterization of Shiga toxin-producing Escherichia coli and enteropathogenic E. coli from livestock in Jiangsu by using whole-genome sequencing

Yue-hong

Liu

Han

et al. 2022

Journal of Applied Microbiology

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Aims: There are knowledge gaps regarding STEC and EPEC strains in livestock in Jiangsu, China. This study aimed to evaluate the potential public health significance of STEC and EPEC strains isolated from livestock by determining the serotypes, virulence profiles, and genetic relationship with international STEC strains. Methods and Results:A total of 68 STEC and 37 EPEC strains were obtained from 231 faecal sheep samples and 70 faecal cattle samples. By using whole-genome sequencing (WGS) analysis, all STEC belonged to 15 O: H serotypes, and the most prevalent serotypes were O6:H10 (19.1%), O155:H21 (14.7%), and O21:H25 (10.3%). The main Shiga toxin gene subtypes detected were stx1c (41.2%), stx1a (26.5%), stx2b (14.7%), and stx2k (14.7%). Only the STEC from cattle carried eae gene. Other adherence-associated or toxin-related genes, including lpfA (70.6%), iha (48.5%), subA (54.4%), and ehxA (33.8%), were found in STEC. All EPEC strains were bfpA-negative, and the predominant eae variants were eae-β1 (62.2%), eae-ζ (21.6%), and eae-θ (8.1%). The core-genome multilocus sequence typing (cgMLST) analysis revealed nine scattered clusters in STEC and one dominant cluster in EPEC. The strains with the same serotypes, including O22:H8 and O43:H2 in the two towns, possessed a closely genomic distance. The core genome single-nucleotide polymorphism (cgSNP) showed that part of STEC strains in this study was clustered with isolates possessing the same serotypes from the Netherlands, Sweden, and Xinjiang of China. Five serotypes of STEC isolates were associated with the clinical STEC strains from databases. Conclusion:This study provided the diverse serotypes and the virulence genes profiles in STEC and EPEC strains. Local strains possessed widely diverse and scattered clusters by cgMLST. Closely genomic correlation with clinical isolates displayed that part of the STEC strains may threaten to public health.Significance and Impact of the Study: Non-O157 STEC strains act as important pathogens for human infections. This study supports the increased surveillance work of non-O157 STEC rather than just O157 STEC in this region.

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Section: Discussionmentioning

confidence: 99%

Genetic and phylogenetic characterization of Shiga toxin-producing Escherichia coli and enteropathogenic E. coli from livestock in Jiangsu by using whole-genome sequencing

Yue-hong

Liu

Han

et al. 2022

Journal of Applied Microbiology

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“…Animals excrete it via the saliva and faeces (26). VTEC is one of the main pathogens in humans; it can cause gastrointestinal dysfunction and haemolytic uremic syndrome (HUS) (9,42,84).…”

Section: Infections Of Verocytotoxigenic Escherichia Coli (Vtec Stec)mentioning

confidence: 99%

“…Beef carcasses can become contaminated when slaughtered, either directly from faeces, or from skin contaminated with faeces; such contamination has clear food safety implications, as the carcasses are further processed into beef products (2,52,53,64). In 2020, testing by the ISO/TS 13136 standard found the VTEC toxin to be present in 115/330 (34.8%) and 37/120 (30.8%) of samples from cattle and pigs (84). Zoonotic transmission occurs via the faecal-oral route, with humans becoming infected by eating contaminated food, or through contact with infected people, infected animals or the environment (26).…”

Section: Zoonotic Potential Of These Diseasesmentioning

confidence: 99%

Zoonotic diseases of potential concern among alpacas MONIKA

Krajewska‐Wędzina¹,

ORŁOWSKA²,

Pyziel-Serafin³

et al. 2022

Medycyna Weterynaryjna

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The aim of the present article is to describe the specific nature of the zoonoses associated with alpacas. Of the potential zoonoses, it is known that tuberculosis (TB), cryptosporidiosis and dermal mite have been transmitted to humans. In addition, associations have been noted between the South American Camelids (SAC) and Escherichia coli human verocytotoxigenic infections (VTECs). The zoonotic potential of TB in camelids primarily poses a threat to veterinarians and owners, it can also be transmitted to the rest of society, as although only a limited number of camelids enter the food chain, alpacas are a very popular in public shows, exhibitions and festivals. Such close contact offers many opportunities to infect humans or other farm animals. The authors presented the potential routes of infection and discussed the clinical symptoms of individual zoonoses in both alpacas and humans.

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Occurrence and genetic characterization of Shiga toxin-producing Escherichia coli on bovine and pork carcasses and the environment from transport trucks

Colello

Baigorri

Canto

et al. 2023

World J Microbiol Biotechnol

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Identification and molecular characteristics of verotoxin-producing Escherichia coli (VTEC) from bovine and pig carcasses isolated in Poland during 2014–2018

Cited by 7 publications

References 55 publications

Genetic and phylogenetic characterization of Shiga toxin-producing Escherichia coli and enteropathogenic E. coli from livestock in Jiangsu by using whole-genome sequencing

Genetic and phylogenetic characterization of Shiga toxin-producing Escherichia coli and enteropathogenic E. coli from livestock in Jiangsu by using whole-genome sequencing

Zoonotic diseases of potential concern among alpacas MONIKA

Occurrence and genetic characterization of Shiga toxin-producing Escherichia coli on bovine and pork carcasses and the environment from transport trucks

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