Identification and quantification of SALATRIM 23CA in foods by the combination of supercritical fluid extraction, particle beam LC-mass spectrometry, and HPLC with light-scattering detector

Normal-phase high performance liquid chromatographic separation and characterization of short- and long-chain triacylglycerols

“…This is in contrast to results obtained on reversed-phase columns [4] NPsiI-HPLC chromatogram of an SLCT obtained by chemical interesterification of hydrogenated soybean oil and triacetin and tripropionin at a l:ll:l ratio (% by weight). With further modifications, we have now found that the method is additionally able to separate SLCT containing the short-chain acids butyric, propionic and acetic.…”

“…The SLCT are well separated from accompanying hydrolysis products of the SO substrate resulting in the appearance of long-chain free fatty acids, diacylglycerols (DAG) and monoacylglycerols (MAG). Compared to the reverse phase HPLC chromatographic procedure published by Huang et al [4], where acetyl-distearoylgtycerol eluted at t R ---50 min, the separation of the TAG molecules in 23SO with the NPcN system was achieved in 9 minutes, but in reverse elution order of SLCT. The sensitivity of the ELSD under the optimum detector conditions, 40 ~ at a nitrogen flow of 1.5 L min -1, was not sufficient enough to detect triacetin.…”

“…As previously shown [4], quantification approaches utilizing HPLC with ELSD detection were not entirely linear using tributyrin but proportional to some power of the concentration of the corresponding compound due to its partial volatility under the conditions used. A SLCT calibration curve was established from the peak area ratio of total 23SO peak areas to the internal standard tributyrin (data not shown).…”

“…For example, Huang et al [4] developed a method for the identification and quantification of SLCT from canola oil and triacetin/tripropionin in foods after total fat extraction by supercritical fluid extraction (SFE) with carbon dioxide. For example, Huang et al [4] developed a method for the identification and quantification of SLCT from canola oil and triacetin/tripropionin in foods after total fat extraction by supercritical fluid extraction (SFE) with carbon dioxide.…”

Purification and Structural Determination of Hematopoietic Stem Cell-Stimulating Monoacetyldiglycerides from Cervus nippon (Deer Antler)

“…16,17) The structures of several monoacetyldiglycerides have been confirmed by gas chromatography (GC), GC-mass spectroscopy (MS), high-performance liquid chromatography (HPLC), liquid chromatography (LC)-MS, nuclear magnetic resonance (NMR), infrared (IR), and single-crystal X-ray analysis. [16][17][18][19] Monoacetyldiglycerides were previously synthesized from corresponding phosphatidylethanolamines, phosphatidylcholines, phosphatidylglycerols, and diacylglycerols by acetolysis and enzymolysis followed by acetylation or esterification. [20][21][22] The water extract of deer antler exhibited interesting growth-promotion effects on the hematopoietic system in our preliminary in vitro experiment, and hence several fractionation and purification steps were utilized to search for the underlying active components.…”